Synergistic Influences of Mentha Piperita and Clinoptilolite: A Study on Histologic and Morphometric Outcomes

Document Type : Original Articles

Authors

Department of Anatomy and Medicinal Plants Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

10.22092/ari.2024.363178.2817

Abstract

Effective wound healing with the highest quality is an important issue to be considered to restore the healthy function of human skin in the early stages. While past research examined the individual impacts of Mentha Piperita and clinoptilolite, this experiment demonstrates their synergistic effects, presenting a promising new approach to expedite wound recovery. The study utilized 60 male Wistar rats, divided into five groups: negative control, positive control (phenytoin ointment), clinoptilolite, M. Piperita, and M. Piperita + clinoptilolite. Ethical considerations were upheld, and neck wounds were created. Histological examinations were conducted on days 4, 7, and 14. Collagen deposition, inflammation, and re-epithelialization scores were also evaluated on day 14 utilizing Abramov’s scoring system. Wound healing assays were performed on days 4, 7, 10, and 14. A P-value <0.05 indicated statistical significance. The M. Piperita + clinoptilolite group exhibited a statistically significantly higher number of fibroblasts and vessels, along with a lower inflammatory cell count compared to all other experimental groups within the wound site. Additionally, all groups demonstrated significantly higher collagen deposition and re-epithelialization scores (P-value <0.05) and lower inflammation scores (not statistically significant) than the control group. Moreover, the M. Piperita + clinoptilolite group showed a significantly smaller wound surface and faster healing rate than all other groups on all experiment day, achieving a remarkable 99.75 ± 0.5 recovery percentage on day 14. The combination of clinoptilolite and M. piperita significantly influences wound healing (considering the histopathologic parameters and clinical outcomes) which is more effective than their separate usage or phenytoin.

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Archives of Razi Institute

Articles in Press, Accepted Manuscript
Available Online from 11 November 2024

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