The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain

Document Type: Original Articles


1 Department of Genetics, Islamic Azad University, Ahar, Iran.

2 Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran

3 Head of viral vaccine QC laboratory

4 Quality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.

5 Department of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

6 Management of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

7 Research and diagnosis of poultry diseases management of Razi Vaccine and Serum Research Institute, Karaj, Iran.

8 Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran

9 Imam Khomeini Hospital Cancer Institute, Tehran, Iran


Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.


Article Title [French]

Détermination des caractéristiques d’une lignée cellulaire d'origine lymphoïde de moutons sensibles à la souche vaccinale d’un virus de la bursite infectieuse des volailles

Abstract [French]

La bursite infectieuse (maladie de Gumboro) a été rapportée de partout dans le monde et l'importance socio-économique de cette maladie est considérable au niveau international. Plusieurs types de vaccins commerciaux ont été introduits pour contrôler cette maladie. Ces vaccins sont produits par culture cellulaire ou dans des œufs embryonnés. Si les vaccins à base de cellules contre la maladie de Gumboro s’avèrent sans danger et efficaces, ils seront beaucoup plus abordables pour le traitement de la maladie. Les fibroblastes embryonnaires de poussin (CEF) sont les cellules les plus populaires pour la production du vaccin contre la Bursite infectieuse par culture cellulaire. Le procès de préparation et d'utilisation des cellules CEF est très long et difficile. Dans cette études, l’attention est portée pour la première fois sur la référence Annex3 TRS 978, WHO, une cellule en suspension de moutons d'origine lymphoïde (H1 Cell Line) ayant montré une sensibilité au virus vaccinal de Gumboro. Cette cellule a passé avec succès tous les tests et a été adaptée à la réplication du virus de la Bursite infectieuse.

Keywords [French]

  • Cellule embryonnaire fibroblastique de poussin
  • Bursite infectieuse des volailles
  • Caractérisation des cellules
  • Lignée cellulaire lymphoïde de mouton
  • La maladie de Gumboro
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