%0 Journal Article %T The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain %J Archives of Razi Institute %I Razi Vaccine & Serum Research Institute %Z 0365-3439 %A Zandieh, S. %A Lotfi, Mohsen %A Kamalzadeh, M. %A Shiri, N. %A Parmour, E. %A Eshaghi, A. %A Masoudi, S. %A Hablolvarid, M. H. %A Shoushtari, A. %A Goudarzi, H. %A Taher Mofrad, S. M. J. %A Amanpour, S. %D 2017 %\ 09/01/2017 %V 72 %N 3 %P 173-179 %! The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain %K Infectious bursal disease %K Gumboro Disease %K Chicken embryo fibroblast %K Ovine lymphoid origin cell line %K Cell characterization %R 10.22092/ari.2017.111601 %X Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale. %U https://archrazi.areeo.ac.ir/article_111601_97eff51c98a27271fec42421e8fd8759.pdf