Assessment of Gill Pathological Responses in Yellowfin Sea Bream (Acanthopagrus Latus) Under Aeromonas Hydrophila Exposure

Document Type: Short Communication


Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khouzestan, Iran


Bacterial diseases in cultured fish are considered the main problem with aquaculture system in Iran. The gills are multifunctional organs responsible for respiration, osmoregulation, nitrogenous waste excretion, and acid-base balance. Moreover, they are very sensitive to water contamination. Aeromonas hydrophila (A. hydrophila) is an opportunist pathogen responsible for a wide range of diseases in different species of fish. The gill histological alterations were used to assess the effects of A. hydrophila exposure on yellowfin sea bream, Acanthopagrus latus (A. latus). In this regard, 90 A. latus were exposed to the concentrations of A. hydrophila (103 and 106 CFU/ml) for three weeks. The most histopathological alterations in the gill of the exposed fish included hypertrophy and hyperplasia of the epithelial cells, lamellar fusion, club shaping of gill lamellae, lifting of the epithelium and edema of lamellae with large sub-epithelial space, blood congestion, and hypertrophy and hyperplasia of the mucosal cells. The histopathological alterations were observed in the gill of fish exposed to higher levels of A. hydrophila (106 CFU/ml) consisted of aneurysm and hemorrhage with blood congestion. According to the obtained results of this study, A. hydrophila could cause severe histopathological changes in the gill of A. latus and decrease gas change capability in yellowfin sea bream. Furthermore, the findings of the present study suggested that histopathological changes of the gill provide helpful information about the environmental conditions and as particular biomarkers may help the evaluation of fish general health.


Main Subjects

Article Title [French]

Évaluation des réponses pathologiques des branchies chez la pagre à nageoires jaunes (Acanthopagrus latus) exposée à Aeromonas hydrophila

Abstract [French]

Les maladies bactériennes chez les poissons d'élevage sont considérées comme le principal problème du système d'aquaculture en Iran. Les branchies sont des organes multifonctionnels responsables de la respiration, de l'osmorégulation, de l'excrétion des déchets azotés et de l'équilibre acido-basique chez le poissonet sont très sensibles à la contamination de l'eau. Aeromonas hydrophila (A. hydrophila) est un agent pathogène opportuniste responsable d'un large éventail de maladies chez différentes espèces de poissons. Les modifications histologiques des branchies ont été utilisées pour évaluer les effets de l'exposition à A. hydrophila sur le pagre à nageoires jaunes, Acanthopagrus latus (A. latus). À cet égard, 90 poissons de l’espèce A. latus ont été exposés à diférenres concentrations d'A. Hydrophila (103 et 106 CFU/ml) pendant trois semaines. Les altérations histopathologiques les plus importantes au niveai deses branchies des poissons exposés incluaient l'hypertrophie et l'hyperplasie des cellules épithéliales, la fusion lamellaire, la formation de lamelles de branchies, la levée de l'épithélium et l'œdème des lamelles avec un grand espace sous-épithélial, la congestion sanguine et l'hypertrophie et l’hyperplasie des cellules muqueuses. D’importantes altérations histopathologiques ont été observées dans les branchies de poissons exposés à des taux plus élevés d'A. hydrophila (106 CFU/ml), qui consistaient en un anévrisme et une hémorragie avec congestion sanguine. Selon les résultats de cette étude, A. hydrophila pourrait provoquer de graves changements histopathologiques dans les branchies de l’A. latus et diminuer la capacité de changement de gaz chez le pagre à nageoires jaunes. En outre, les résultats de la présente étude suggèrent que les modifications histopathologiques des branchies fournissent des informations utiles sur les conditions environnementales et que des biomarqueurs particuliers peuvent aider à évaluer la santé générale des poissons.

Keywords [French]

  • Histopathologie
  • branchie
  • Acanthopagrus latus
  • Aeromonas hydrophila
Abdelhamed, H., Ibrahim, I., Nho, S.W., Banes, M.M., Wills, R.W., Karsi, A., et al., 2017. Evaluation of three recombinant outer membrane proteins, OmpA1, Tdr, and TbpA, as potential vaccine antigens against virulent Aeromonas hydrophila infection in channel catfish (Ictalurus punctatus). Fish Shellfish Immunol 66, 480-486.

Arunkumar, R.I., Rajasekaran, P., Michael, R.D., 2000. Differential effect of chromium compounds on the immune response of the African mouth breeder Oreochromis mossambicus (Peters). Fish Shellfish Immunol 10, 667-676.

Austin, B., 2006. The bacterial microflora of fish, revised. The Scientific World Journal 6, 931-945.

Boas, M., Feldt-Rasmussen, U., Skakkebæk, N.E., Main, K.M., 2006. Environmental chemicals and thyroid function. Eur J Endocrinol 154, 599-611.

Brown, S.B., Adams, B.A., Cyr, D.G., Eales, J.G., 2004. Contaminant effects on the teleost fish thyroid. Environ Toxicol Chem 23, 1680-1701.

Carletta, M., Weis, P., Weis, J., 2002. Development of thyroid abnormalities in mummichogs, Fundulus heteroclitus, from a polluted site. Marine Environ Res 54, 601-604.

Harikrishnan, R., Balasundaram, C., 2005. Modern trends in Aeromonas hydrophila disease management with fish. Rev Fish Sci 13, 281-320.

Harikrishnan, R., Rani, M.N., Balasundaram, C., 2003. Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture 221, 41-50.

Hesp, S.A., Potter, I.C., Hall, N.G., 2004. Reproductive biology and protandrous hermaphroditism in Acanthopagrus latus. Environ Biol Fish 70, 257-272.

Jiraungkoorskul, W., Upatham, E.S., Kruatrachue, M., Sahaphong, S., Vichasri-Grams, S., Pokethitiyook, P., 2002. Histopathological effects of Roundup, a glyphosate herbicide, on Nile tilapia (Oreochromis niloticus). Sci Asia 28, 121-127.

Khalil, A., Mansour, E., 1997. Toxicity of crude extracellular products of Aeromonas hydrophila in tilapia, Tilapianilotica. Lett Appl Microbiol 25, 269-273.

Kumar, R., Pande, V., Singh, L., Sharma, L., Saxena, N., Thakuria, D., et al., 2016. Pathological findings of experimental Aeromonas hydrophila infection in golden mahseer (Tor putitora). Fish Aquacult J 7, 160.

Mohamed, F.A., 2009. Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt. World J
Fish Marine Sci 1, 29-39.

Oliveira, C.R., Fanta, E., Turcatti, N., Cardoso, R., Carvalho, C., 1996. Lethal effects of inorganic mercury on cells and tissues of Trichomycterus brasiliensis (Pisces; Siluroidei). Biocell 20, 171-178.

Poleksić, V., Mitrović-Tutundžić, V., 1994. Fish gills as a monitor of sublethal and chronic effects of pollution. Sublethal and chronic effects of pollutants on freshwater fish. Oxford: Fishing News Books, 339-352.

Rauta, P.R., Nayak, B., Monteiro, G.A., Mateus, M., 2017. Design and characterization of plasmids encoding antigenic peptides of Aha1 from Aeromonas hydrophila as prospective fish vaccines. J Biotechnol 241, 116-126.

Rodríguez, I., Novoa, B., Figueras, A., 2008. Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila. Fish Shellfish Immunol 25, 239-249.

Sahoo, P.K., Mahapatra, K.D., Saha, J.N., Barat, A., Sahoo, M., Mohanty, B.R., et al., 2008. Family association between immune parameters and resistance to Aeromonas hydrophila infection in the Indian major carp, Labeo rohita. Fish Shellfish Immunol 25, 163-169.

Sahoo, P.K., Pillai, B.R., Mohanty, J., Kumari, J., Mohanty, S., Mishra, B.K., 2007. In vivo humoral and cellular reactions, and fate of injected bacteria Aeromonas hydrophila in freshwater prawn Macrobrachium rosenbergii. Fish Shellfish Immunol 23, 327-340.

Schlenk, D., Benson, W.H., 2003. Target Organ Toxicity in Marine and Freshwater Teleosts: Organs, Taylor & Francis.

Suvarna, S.K., Layton, C., Bancroft, J.D., 2013. Bancroft's Theory and Practice of Histological Techniques, Expert Consult: Online and Print, 7: Bancroft's Theory and Practice of Histological Techniques, Churchill Livingstone Elsevier.

Yun, S., Jun, J.W., Giri, S.S., Kim, H.J., Chi, C., Kim, S.G., et al., 2017. Efficacy of PLGA microparticle-encapsulated formalin-killed Aeromonas hydrophila cells as a single-shot vaccine against A. hydrophila infection. Vaccine 35, 3959-3965.