Document Type : Original Articles
Authors
1
Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2
PhD Student of Virology Department of Microbiology and Immunology Faculty of Veterinary Medicine University of Tehran
3
Department of Nutrition, Dietetics and food, Faculty of science, Science and Research Branch, Islamic Azad University, Tehran, Iran
4
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5
Department of Food Health and Hygiene, Veterinary Organization of Tehran Province, Iran Veterinary Organization, Tehran, Iran
6
Department of Virology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
7
69 Azadi Ave
10.66224/ari.2026.362523.2751
Abstract
Salmonellosis is a significant zoonotic disease that causes foodborne poisoning in humans through animal products and is considered a worldwide public health hazard. A significant hurdle in managing and preventing salmonellosis lies in accurately identifying individuals who may act as asymptomatic carriers, potentially spreading the pathogen without showing clinical signs. This study aimed to assess the prevalence, serotype distribution, and antimicrobial resistance profiles of Salmonella isolates recovered from duck eggs. Fifty-four local duck eggs were randomly obtained from villagers in northern Iran during a one-week sampling period in winter 2021 and subsequently transported to the Microbiology Laboratory. Salmonella was isolated according to standard culture and biochemical tests. The Salmonella positive samples were serotyped with O and H antisera based on slide and tube agglutination tests. Genus-specific (invA) PCR for the identification of Salmonella spp. Then, PCR was done for detection of serovars Infantis and Enteritidis. The antibiotic resistance against 17 antimicrobial agents was tested. Results indicated 11.11% (6 of 54) of duck eggs were positive for Salmonella in bacterial isolation. Serotyping results showed one sample was serovar Infantis (1.85%) and five samples were serovar Enteritidis (9.25%), and PCR confirmed the serotyping results. Most of the Salmonella isolates in this study were sensitive to ciprofloxacin, kanamycin, norfloxacin, lincospectine, sultrim, chloramphenicol, cephalothin, flumequine, florfenicol, gentamicin, nitrofurantoin, co-amoxiclav, enrofloxacin, and cefotaxime and resistant to ampicillin, oxytetracycline, and tetracycline. The presence of Salmonella infections among duck eggs revealed that duck eggs could also be potential reservoirs for Salmonella and should draw particular attention in terms of both human and animal health.
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