Department of Biology, NT.C., Islamic Azad University, Tehran, Iran
10.22092/ari.2025.369700.3686
Abstract
Colorectal cancer is one of the most common cancers worldwide , particularly in developing countries. Biomarkers such as long non coding RNAs ( lncRNAs ) play a pivotal role in early detection and personalized treatment. This study aimed to compare the expression levels of PVT 1 lncRNA and TGF β gene in colorectal cancer tissues and non cancerous polyp tissues. Fifty colorectal cancer tissue samples and fifty non cancerous polyp tissues, confirmed by a gastroenterologist, were analyzed. RNA was extracted, cDNA was synthesized, and expression levels of PVT 1 lncRNA and TGF-β were quantified using real-time PCR. Data analysis was performed with SPSS software, and all experiments were conducted under identical laboratory conditions to ensure reliability. The colorectal cancer group included 22 males and 28 females ( mean age: 62.56 ± 16.41 years ), while the control group consisted of 30 males and 20 females ( mean age: 58.41 ± 11.13 years ). Expression analysis revealed significantly higher levels of both PVT 1 lncRNA ( fold change = X.X, P < 0.05 ) and TGF-β ( fold change = X.X, P < 0.05 ) in colorectal cancer tissues compared to controls. These differences persisted despite demographic imbalances between groups. ROC curve analysis demonstrated the diagnostic potential of both biomarkers in distinguishing cancerous from non cancerous tissues. Our findings suggest that PVT 1 lncRNA and TGF-β may serve as promising molecular biomarkers for colorectal cancer, with potential diagnostic utility. Further validation studies are warranted to establish their role in clinical practice.
Aminzare, F. , Mansoubi, S. , Zare Tooranposhti, Z. and Mohsenpour, M. (2025). Expression Analysis of PVT-1 lncRNA and TGF-β in Colorectal Cancer and Non-Cancerous Colorectal Polyps: A Case-Control Study. Archives of Razi Institute, (), -. doi: 10.22092/ari.2025.369700.3686
MLA
Aminzare, F. , , Mansoubi, S. , , Zare Tooranposhti, Z. , and Mohsenpour, M. . "Expression Analysis of PVT-1 lncRNA and TGF-β in Colorectal Cancer and Non-Cancerous Colorectal Polyps: A Case-Control Study", Archives of Razi Institute, , , 2025, -. doi: 10.22092/ari.2025.369700.3686
HARVARD
Aminzare, F., Mansoubi, S., Zare Tooranposhti, Z., Mohsenpour, M. (2025). 'Expression Analysis of PVT-1 lncRNA and TGF-β in Colorectal Cancer and Non-Cancerous Colorectal Polyps: A Case-Control Study', Archives of Razi Institute, (), pp. -. doi: 10.22092/ari.2025.369700.3686
CHICAGO
F. Aminzare , S. Mansoubi , Z. Zare Tooranposhti and M. Mohsenpour, "Expression Analysis of PVT-1 lncRNA and TGF-β in Colorectal Cancer and Non-Cancerous Colorectal Polyps: A Case-Control Study," Archives of Razi Institute, (2025): -, doi: 10.22092/ari.2025.369700.3686
VANCOUVER
Aminzare, F., Mansoubi, S., Zare Tooranposhti, Z., Mohsenpour, M. Expression Analysis of PVT-1 lncRNA and TGF-β in Colorectal Cancer and Non-Cancerous Colorectal Polyps: A Case-Control Study. Archives of Razi Institute, 2025; (): -. doi: 10.22092/ari.2025.369700.3686
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