Phylogenetic relationships of the scorpion Apistobuthus susanae from Khuzestan province based on mitochondrial DNA cytochrome oxidase I (COXI) gene sequences
1
Department of Faculty of Engineering and Agriculture, Arak Branch, Islamic Azad University, Arak, Iran.
2
Razi Vaccine and Serum Research Institute: Ahvaz, Khuzestan, Iran
10.22092/ari.2025.368866.3575
Abstract
This study aims to analyze the morphometric and phylogeny of Apistobuthus susanae scorpions using mitochondrial DNA sequences. Information regarding the morphology and biology of scorpions is very limited, and the foundation of this information is the identification of habitats, morphology, and morphometric of scorpions in different regions. In this study, the morphometric study based on 32 morphological characteristics of A. susanae and DNA sequencing of COXI was performed. Phylogenetic tree done by wing MEGA 10 software. Both male and female specimens of A. susanae were collected from four cities: Hamidiyeh, Masjed Soleyman, Ramhormoz, and Andimeshk in Khuzestan province. According to the results, in all cases, the average sizes of female specimens were larger than those of the male specimens. Except for the traits Cl, CHL, ML, TIW, MTIL, MTIILL, MTIVL, MTIIW, MTIVW, and MTIH, all other features studied were identical in both male and female species (P > 0.05). The results of the analysis of morphometric values of the A. susanae were compatible with the phylogenetic tree and supported the morphometric classification. Out of 614 nucleotides of the COXI gene amplified for 10 Apistobuthus samples and one sample of Androctonus crassicauda as outgroup, 558 sites were conserved (90.97%), 41 sites were variable (6.76%), and 15 sites (2.25%) had parsimony-informative sites. The analysis of the average genetic distance within species showed that two specimens from Hamidiyeh had the least divergence (0%) and two specimens from Ramhormoz and Andimeshk had the greatest inter-species genetic divergence (0.1%). The greatest intraspecific divergence was observed in the specimens from Andimeshk and Masjed Soleyman, while the least genetic divergence was found in the Ramhormoz samples
bahri, S. , shams esfandabadi, B. , ahmadi, A. and Jafari, H. (2025). Phylogenetic relationships of the scorpion Apistobuthus susanae from Khuzestan province based on mitochondrial DNA cytochrome oxidase I (COXI) gene sequences. Archives of Razi Institute, (), -. doi: 10.22092/ari.2025.368866.3575
MLA
bahri, S. , , shams esfandabadi, B. , , ahmadi, A. , and Jafari, H. . "Phylogenetic relationships of the scorpion Apistobuthus susanae from Khuzestan province based on mitochondrial DNA cytochrome oxidase I (COXI) gene sequences", Archives of Razi Institute, , , 2025, -. doi: 10.22092/ari.2025.368866.3575
HARVARD
bahri, S., shams esfandabadi, B., ahmadi, A., Jafari, H. (2025). 'Phylogenetic relationships of the scorpion Apistobuthus susanae from Khuzestan province based on mitochondrial DNA cytochrome oxidase I (COXI) gene sequences', Archives of Razi Institute, (), pp. -. doi: 10.22092/ari.2025.368866.3575
CHICAGO
S. bahri , B. shams esfandabadi , A. ahmadi and H. Jafari, "Phylogenetic relationships of the scorpion Apistobuthus susanae from Khuzestan province based on mitochondrial DNA cytochrome oxidase I (COXI) gene sequences," Archives of Razi Institute, (2025): -, doi: 10.22092/ari.2025.368866.3575
VANCOUVER
bahri, S., shams esfandabadi, B., ahmadi, A., Jafari, H. Phylogenetic relationships of the scorpion Apistobuthus susanae from Khuzestan province based on mitochondrial DNA cytochrome oxidase I (COXI) gene sequences. Archives of Razi Institute, 2025; (): -. doi: 10.22092/ari.2025.368866.3575
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