Department of microbiology and immunology, Faculty of veterinary medicine, University of Tehran, Tehran, Iran
10.22092/ari.2025.368672.3585
Abstract
The feline panleukopenia virus (FPV), a member of the Parvoviridae family, is a major pathogen in cats, and is associated with a high risk of mortality and morbidity. Infection in cats can cause severe leukopenia and gastroenteritis, and even lead to nervus signs. In the present study, during September 2022 to September 2023, 31 blood and fecal samples were collected from cats with clinical symptoms of feline panleukopenia from four different veterinary clinics/hospitals in Gilan, Mazandaran, and Tehran provinces. Reports of Rapid test antigenic detection kit were also collected for cases (if performed) from clinic and hospitals. The presence of FPV was assessed by molecular assay using PCR method. PCR was performed using specific primers to amplify a partial VP2 protein encoding gene, and the positive samples were subsequently submitted for sequencing. According to the phylogenetic tree generated by MEGA 7 software using the Maximum Likelihood method, samples in this study were clustered with previously submitted FPV isolates and based on homology analysis current samples are similar to previously Iranian isolate, IR-FPV2014.2 which has been obtained from a domestic cat in Tehran in 2014. Homology results also showed high similarity of current isolate with isolate CU4 which used as reference, isolate C14 isolated in Nigeria from domestic cat and isolate “FPV/Raccoon/NJ/RPV-6/90” isolated from Procyon lotor (Racoon) in United States. According to the rapid test kit results, 78.57% of indoor cats and 42.86% of outdoor cats were positive for FPV infection. PCR results also revealed that 61.11 % of indoor cats and 30.77% of outdoor cats were infected with FPV. More molecular and epidemiologic studies are recommended to clarify the actual prevalence of FPV in Iran and the infection status among indoor cats and stray cats population.
Mehinparvar Irani, D. , Sadri, N. , Ziafati Kafi, Z. , Sarmadi, S. , Abbassioun, A. , Jamiri, F. and Ghalyanchilangeroudi, A. (2025). Molecular detection and characterization of feline parvovirus in cats in Iran. Archives of Razi Institute, (), -. doi: 10.22092/ari.2025.368672.3585
MLA
Mehinparvar Irani, D. , , Sadri, N. , , Ziafati Kafi, Z. , , Sarmadi, S. , , Abbassioun, A. , , Jamiri, F. , and Ghalyanchilangeroudi, A. . "Molecular detection and characterization of feline parvovirus in cats in Iran", Archives of Razi Institute, , , 2025, -. doi: 10.22092/ari.2025.368672.3585
HARVARD
Mehinparvar Irani, D., Sadri, N., Ziafati Kafi, Z., Sarmadi, S., Abbassioun, A., Jamiri, F., Ghalyanchilangeroudi, A. (2025). 'Molecular detection and characterization of feline parvovirus in cats in Iran', Archives of Razi Institute, (), pp. -. doi: 10.22092/ari.2025.368672.3585
CHICAGO
D. Mehinparvar Irani , N. Sadri , Z. Ziafati Kafi , S. Sarmadi , A. Abbassioun , F. Jamiri and A. Ghalyanchilangeroudi, "Molecular detection and characterization of feline parvovirus in cats in Iran," Archives of Razi Institute, (2025): -, doi: 10.22092/ari.2025.368672.3585
VANCOUVER
Mehinparvar Irani, D., Sadri, N., Ziafati Kafi, Z., Sarmadi, S., Abbassioun, A., Jamiri, F., Ghalyanchilangeroudi, A. Molecular detection and characterization of feline parvovirus in cats in Iran. Archives of Razi Institute, 2025; (): -. doi: 10.22092/ari.2025.368672.3585
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