Document Type : Original Articles
Authors
1
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2
Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
3
Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran
4
Department of Biochemistry , Falavarjan Branch, Islamic Azad University, Isfahan, Iran
10.22092/ari.2025.368152.3485
Abstract
Due to the prevalence of MDR strains, alternative treatments, such as antimicrobial photodynamic therapy (aPDT), have received much attention. This technique is an interactive technology that works with the help of photosensitizers and through the production of active oxygen radicals. This study aimed to investigate the effectiveness of aPDT against multi-drug resistant P. aeruginosa bacteria using nanoemulsion containing zinc phthalocyanine (NE/ ZnPc) in a burn wound model in in vitro and in vivo conditions.
Optimization of photodynamic therapy conditions in different drug concentrations, different incubation times, different doses of laser radiation was performed in vitro, and then the anti-biofilm effects of the optimal concentration against clinical isolates of P. aeruginosa resistant to several drugs were investigated. In vivo conditions, male rats were divided in 7 groups: control, burn wound group, bacterial infected burn wound, infected burn wound and laser treatment, infected wound and treatment with laser and drug (optimal concentration), infected wound and treatment with drug alone, infected wound and treatment with nanoemulsion alone. The area of the burn wound was measured at the beginning and end of the test. Pieces of skin tissue were collected in order to perform histological studies and measure the expression of EGFR and SOD1 genes.
Based on the results, the drug in concentration of 40 μg/ml encapsulated in nanoemulsion , the laser dose of 13 J/cm2 and the incubation time of 2 hours were selected as optimal conditions. Under these conditions, 80% biofilm inhibition was observed. According to the histological results, NE/ ZNPc-mediated aPDT and laser radiation reduced the burn wound area and significantly accelerated the wound healing process, as well as increasing EGFR gene expression and decreasing SOD1 gene expression to normal levels.
the promising antibacterial and anti-biofilm activity of NE/ClZNlPc-mediated aPDT against MDR P. aeruginosa bacteria and the increased expression of EGFR and SOD1 in the infected wounds of rats indicate that this treatment may be used as an alternative treatment to eradicate bacteria with Multidrug resistance.
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