Anatomical and histological investigation of the effect of superoxide dismutase on eye muscles in Tuj sheep

Kirbaş Doğan, Gülseren; Eliş Yildiz, Sevda; Karadağ Sari, Ebru; Ölmez, Mükremin; Şahin, Tarkan

doi:10.32592/ARI.2025.80.4.879

Anatomical and histological investigation of the effect of superoxide dismutase on eye muscles in Tuj sheep

Document Type : Original Articles

Authors

¹ Department of Anatomy, Faculty of Veterinart Medicine, University of Kafkas, Kars, Turkey.

² Department of Midwifery, Health Sciences Faculty, Kafkas University, Kars, Turkey.

³ Department of Histology and Embryology, Veterinary Faculty, Kafkas University, Kars, Turkey.

⁴ Department of Animal Nutrition and Nutritional Diseases, Kafkas University, Kars, Turkey.

10.32592/ARI.2025.80.4.879

Abstract

In the study, 16 eye muscles belonging to 8 male Tuj breed sheep, aged around 2-3 months, were used. The research was carried out on two groups: control and study groups. The mean and standard deviation values of all measurements obtained as a result of anatomical examinations and the differences between the control and study groups were determined by the 'Independent Samples T' test in the SPSS package program. While no additive was added to the control group diet, an antioxidant feed additive rich in superoxide dismutase was added to the study group diet. As a result of anatomical evaluation, the longest muscle in the control group was musculus obliquus dorsalis (34.43 mm) and the shortest muscle was musculus retractor bulbi (21.74 mm). In the study group, the longest muscle was musculus rectus dorsalis (34.5 mm) and the shortest muscle was musculus retracor bulbi (22.27 mm). Mallory's modified triple staining (Triple) was applied for histolohical examination. In our study, it was observed that the musculus rectus dorsalis, musculus rectus ventralis, musculus rectus medialis, musculus rectus lateralis, musculus obliquus dorsalis, musculus obliquus ventralis and musculus retractor bulbi muscles obtained from Tuj sheep in the study and control groups consisted of a striated skeletal muscle system and showed transverse striations. What was clearly seen in both anatomical and histological evaluation was that superoxide dismutase had a positive effect on the musculus rectus medialis, one of the eye muscles. It is thought that the presented study will contribute to intraocular eye surgery operations and research relating eye diseases to nutrition.

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