Molecular detection of virulence genes and multi-drug resistance patterns in Streptococcus agalactiae in clinical bovine mastitis: Tehran and Alborz provinces, Iran

Hashemi Haghighi, Fatemeh; Pourtaghi, Hadi; Harzandi, Naser -; Moosakhani, Farhad -

doi:10.22092/ari.2025.368086.3479

Molecular detection of virulence genes and multi-drug resistance patterns in Streptococcus agalactiae in clinical bovine mastitis: Tehran and Alborz provinces, Iran

Articles in Press

Document Type : Original Articles

Authors

Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

10.22092/ari.2025.368086.3479

Abstract

Streptococcus agalactiae is one of the important causes of mastitis in cows. The ability of Streptococcus agalactiae to cause disease depends on the production of a large number of virulence factors encoded by different genes. The overuse of antibiotics to treat mastitis can lead to antibiotic resistance. This research was conducted to detect some virulence genes and the antibiotic resistance of Streptococcus agalactiae. For this purpose, a total of 30 samples of Streptococcus agalactiae isolated from the milk of different cows presenting clinical mastitis in Tehran and Alborz, out of these, 24 samples were confirmed as Streptococcus agalactiae through the detection of the two 16S-23S rRNA genes. Disk diffusion method for a panel of 10 antimicrobial agents showed a large number of strains resistant simultaneously to six antibiotics. Five virulence genes bac, bca, cylE, hylB, and cfb were screened by polymerase chain reaction (PCR). The cfb and hylB genes were found in 95.83 % of the isolates. cylE gene was detected in 29.16 % of the isolates. bca and bac genes were not detected in any of the isolates. The bac and bca genes likely have minimal impact on the pathogenesis of Streptococcus agalactiae mastitis in dairy cows, while the hylB and cfb genes play a crucial role in this condition. The results presented here are one of the first molecular data concerning these five virulence genes in Streptococcus agalactiae isolates causing bovine mastitis in the Tehran and Alborz provinces that provide a foundation for the development of diagnostic, preventive, and therapeutic methods.

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