Document Type : Original Articles
Authors
1
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2
Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran
3
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran-Iran
4
Department of Human Viral Vaccines, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
10.22092/ari.2024.367394.3386
Abstract
RBK cell line derived from primary bovine kidney cell was established and introduced by Razi Vaccine and Serum Research Institute. RBK cell line has been turned into continuous cell line through serial passaging of primary cell. On the basis of the experiments conducted, RBK cell line demonstrates high sensitivity to viruses. As cell line identification is crucial to confirmation of the experimental results, and can attest to the validity and credibility of such findings. Misidentification of the cell line, misdiagnosis of its contamination or lack thereof, can lead to unreliable results as well as waste of resources. It is important to forecast the value of a cell line as it can determine its origin. The more the explanation of the origin, the more valuable the cell line. In addition, contamination is a serious concern in cell culture experiments as it can affect the outcomes, and result in false +/- faults. Therefore, based on standard routines, it is necessary to establish that the cell line is free contamination.
As for the purposes of identification, verification and authentication of a cell line, various methods must be applied simultaneously to a particular cell line; so, in this study, molecular tests such as PCR, PCR-RFLP and real time PCR were used to determine the degree of certainty in identification and purification of RBK cell line; The present study provides RBK cell line authentication; In addition, the results demonstrate the RBK is free of adventitious agents such as Bovine Leukemia Virus (BLV) and Bovine Viral Diarrhea (BVDV). In consideration of the RBK cell line capability to unlimited proliferation, stability and sensitivity to viruses, the present study introduces this cell line as a reliable tool for viral research, which can contribute to numerous important scientific advances.
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