Probiotic Strategies for Detoxification of AFM1 in Skim Milk Using Bifidobacterium Lactis and Streptococcus Thermophiles

Document Type : Original Articles

Authors

1 Department of Food Hygiene, Qeshm Branch, Islamic Azad University, Qeshm, Iran

2 Department of food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Department of Fisheries, Qeshm Branch, Islamic Azad University, Qeshm, Iran

5 Department of Pathobiology, School of Veterinary Science and Research Branch, Islamic Azad University, Tehran, Iran

10.32592/ARI.2025.80.3.849

Abstract

This study was conducted to evaluate the efficacy of Bifidobacterium lactis and Streptococcus thermophilus, both independently and in combination, in detoxifying skim milk contaminated with aflatoxin M1 (AFM1). To achieve this, two concentrations of the bacteria (8 and 10 log CFU/mL) were inoculated into skimmed milk contaminated with three levels of AFM1 (0.1, 0.25, and 0.5 μg/mL) and incubated at two different temperatures (4 and 42 °C). High-performance liquid chromatography (HPLC) was employed to measure the removal percentage of AFM1 at various intervals (30, 60, 120 minutes, and 24 hours). Results indicated a significant time-dependent increase in AFM1 removal from the skim milk. The removal efficiency of AFM1 by these bacterial strains ranged from 12% to 87%, influenced by bacterial concentration, incubation time, toxin concentration, and whether the bacteria were used alone or in combination. B. lactis exhibited a superior AFM1 removal capacity compared to S. thermophilus. The optimal strategy for maximum AFM1 removal (87%) involved treating contaminated milk spiked with 0.5 μg/mL of AFM1 with a mixture of B. lactis and S. thermophilus at concentrations of 10 and 8 log CFU/mL, respectively, and incubating at 42ºC for 24 hours. This study suggests a potentially effective method for reducing AFM1 concentrations in the dairy industry, thereby mitigating public health risks associated with aflatoxin contamination. The implications of these findings could contribute significantly to improving food safety standards and reducing exposure to harmful toxins in dairy products. Further research is recommended to explore the underlying mechanisms of AFM1 removal by these probiotic strains and to validate these findings under commercial dairy processing conditions.

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  1. Kumar P, Mahato DK, Kamle M, Mohanta TK, Kang SG. Aflatoxins: A global concern for food safety, human health and their management. Frontiers in microbiology. 2017;7:2170.
  2. Jallow A, Xie H, Tang X, Qi Z, Li P. Worldwide aflatoxin contamination of agricultural products and foods: From occurrence to control. Comprehensive reviews in food science and food safety. 2021;20(3):2332-81.
  3. Dohnal V, Wu Q, Kuca K. Metabolism of aflatoxins: key enzymes and interindividual as well as interspecies differences. Archives of toxicology. 2014;88(9):1635-44.
  4. Lima CMG, Costa HRD, Pagnossa JP, Rollemberg NdC, Silva JFd, Dalla Nora FM, et al. Influence of grains postharvest conditions on mycotoxins occurrence in milk and dairy products. Food Science and Technology. 2021;42.
  5. Mollayusefian I, Ranaei V, Pilevar Z, Cabral-Pinto MMS, Rostami A, Nematolahi A, et al. The concentration of aflatoxin M1 in raw and pasteurized milk: A worldwide systematic review and meta-analysis. Trends in Food Science & Technology. 2021;115:22-30.
  6. Vagef R, Mahmoudi R. Occurrence of Aflatoxin M1 in raw and pasteurized milk produced in west region of Iran (during summer and winter). International Food Research Journal. 2013;20(3):1421.
  7. Ismail A, Goncalves BL, de Neeff DV, Ponzilacqua B, Coppa CFSC, Hintzsche H, et al. Aflatoxin in foodstuffs: Occurrence and recent advances in decontamination. Food Research International. 2018;113:74-85.
  8. Goncalves BL, Muaz K, Coppa CFSC, Rosim RE, Kamimura ES, Oliveira CAF, et al. Aflatoxin M1 absorption by non-viable cells of lactic acid bacteria and Saccharomyces cerevisiae strains in Frescal cheese. Food research international. 2020;136:109604.
  9. Pop OL, Suharoschi R, Gabbianelli R. Biodetoxification and Protective Properties of Probiotics. Microorganisms. 2022;10(7):1278.
  10. Corassin CH, Bovo F, Rosim RE, Oliveira CAFd. Efficiency of Saccharomyces cerevisiae and lactic acid bacteria strains to bind aflatoxin M1 in UHT skim milk. Food control. 2013;31(1):80-3.
  11. Mahmood Fashandi H, Abbasi R, Mousavi Khaneghah A. The detoxification of aflatoxin M1 by Lactobacillus acidophilus and Bifidobacterium spp.: A review. Journal of food processing and preservation. 2018;42(9):e13704.
  12. Allam NG, Ali EMM, Shabanna S, Abd-Elrahman E. Protective efficacy of Streptococcus thermophilus against acute cadmium toxicity in mice. Iranian journal of pharmaceutical research: IJPR. 2018;17(2):695.
  13. Ismail A, Levin RE, Riaz M, Akhtar S, Gong YY, de Oliveira CAF. Effect of different microbial concentrations on binding of aflatoxin M1 and stability testing. Food control. 2017;73:492-6.
  14. Sokoutifar R, Razavilar V, Anvar AA, Shoeiby S. Degraded aflatoxin M1 in artificially contaminated fermented milk using Lactobacillus acidophilus and Lactobacillus plantarum affected by some bio-physical factors. Journal of Food Safety. 2018;38(6):e12544.
  15. Bovo F, Corassin CH, Rosim RE, de Oliveira CAF. Efficiency of lactic acid bacteria strains for decontamination of aflatoxin M1 in phosphate buffer saline solution and in skimmed milk. Food and Bioprocess Technology. 2013;6(8):2230-4.
  16. Elsanhoty RM, Salam SA, Ramadan MF, Badr FH. Detoxification of aflatoxin M1 in yoghurt using probiotics and lactic acid bacteria. Food control. 2014;43:129-34.
  17. Pierides M, El-Nezami H, Peltonen K, Salminen S, Ahokas J. Ability of dairy strains of lactic acid bacteria to bind aflatoxin M1 in a food model. Journal of food protection. 2000;63(5):645-50.
  18. Sarlak Z, Rouhi M, Mohammadi R, Khaksar R, Mortazavian AM, Sohrabvandi S, et al. Probiotic biological strategies to decontaminate aflatoxin M1 in a traditional Iranian fermented milk drink (Doogh). Food control. 2017;71:152-9.
  19. Adibpour N, Soleimanian-Zad S, Sarabi-Jamab M, Tajalli F. Effect of storage time and concentration of aflatoxin m1 on toxin binding capacity of L. acidophilus in fermented milk product. Journal of Agricultural Science and Technology. 2016;18(5):1209-20.
  20. Panwar R, Kumar N, Kashyap V, Ram C, Kapila R. Aflatoxin M1 detoxification ability of probiotic lactobacilli of Indian origin in in vitro digestion model. Probiotics and antimicrobial proteins. 2019;11(2):460-9.
  21. Karazhiyan H, Mehraban SM, Karazhyan R, Mehrzad A, Haghighi E. Ability of different treatments of Saccharomyces cerevisiae to surface bind aflatoxin M1 in yoghurt. Journal of Agricultural Science and Technology. 2016;18:1489-98.
  22. Namvar Rad M, Razavilar V, Anvar SAA, Akbari-Adergani B. Selected bio-physical factors affecting the efficiency of Bifidobacterium animalis lactis and Lactobacillus delbrueckii bulgaricus to degrade aflatoxin M1 in artificially contaminated milk. Journal of Food Safety. 2018;38(4):e12463.
  23. El Khoury A, Atoui A, Yaghi J. Analysis of aflatoxin M1 in milk and yogurt and AFM1 reduction by lactic acid bacteria used in Lebanese industry. Food control. 2011;22(10):1695-9.
  24. Barukcic I, Bilandzic N, Markov K, Jakopovic KL, Bozanic R. Reduction in aflatoxin M1 concentration during production and storage of selected fermented milks. International journal of dairy technology. 2018;71(3):734-40.