Preparation of SSCs recipients in sheep; effects of anticancer drugs

Zandi, Mohammad; Ghaedrahmati, Annahita

doi:10.22092/ari.2025.366856.3321

Preparation of SSCs recipients in sheep; effects of anticancer drugs

Articles in Press

Document Type : Original Articles

Authors

¹ Iranian Research Organization for Science and Technology (IROST)

² Department of Animal Science, Agricultural Sciences and Natural Resources University of Khuzestan, Iran

10.22092/ari.2025.366856.3321

Abstract

This in vitro investigation aimed to assess the impact of Taxol, Carboplatin, Vinblastine (VBL), and Vincristine (VCR) on ovine Spermatogonial Stem Cells. Spermatogonial Stem Cells can be extracted from the testes of an animal and cultured in a laboratory to increase their quantity for transplantation into a recipient. Upon transplantation, these cells maintain their population and initiate sperm production in the recipient animal. Adequate preparation of the recipient is a crucial aspect of Spermatogonial Stem cell transplantation. Drawing upon the findings of in vitro investigations conducted on Spermatogonia Stem Cells, a tripartite regimen comprising Carboplatin, Taxol, and VCR was administered to four-month-old male Shal lambs at varying concentrations. The in vivo experiments included culture of Spermatogonia Stem Cells, gfrαl and c-myc specific gene expression analysis, and histological analysis. The results demonstrated that injection of the combination as mentioned above (at concentrations of 2.0632, 0.906, and 0.0072 mg, respectively) beneath the scrotums of each testicle of Shal lambs resulted in a significant reduction in the Spermatogonial Stem Cells population. The findings suggest that a tripartite regimen consisting of Carboplatin, Taxol, and VCR may be suitable for preparing sheep recipients for Spermatogonial Stem Cells, and based on the results of this study, the use of a combination of Carboplatin, Taxol, and Vincristine is recommended for the preparation of SSC transplant receptors in sheep. Injection of a combination of Carboplatin, Taxol, and Vincristine equivalent to twice the concentration recommended for cancer treatment by the drug manufacturer under the Scrotum of each of the testicles of Shal lambs, significantly reduces the cell population. The expression of gfrαl and c-myc genes as specific markers of stem cells was reduced considerably in double and quadruple concentrations of Carboplatin + Taxol + Vincristine compared to other treatments.

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