Antioxidative, Hepatoprotective, and Antidiabetic, Properties of The Chitosan Nanoparticles, Loaded with Hydroalcoholic Extracts of Aerial Part of the Hypericum perforatum L. and Trigonella gracum Seed in Streptozotocin-Induced Diabetic Rats

Mansourian, Mahboubeh; Noroozi, Fatemeh; Ghaedi, Mehrorang; Doustimotlagh, Amirhossein; Badania, Hassan; Azizi, Mahdokht; Kargar Jahromi, Hossein

doi:10.22092/ari.2024.366824.3298

Antioxidative, Hepatoprotective, and Antidiabetic, Properties of The Chitosan Nanoparticles, Loaded with Hydroalcoholic Extracts of Aerial Part of the Hypericum perforatum L. and Trigonella gracum Seed in Streptozotocin-Induced Diabetic Rats

Articles in Press

Document Type : Original Articles

Authors

¹ Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.

² Department of Chemistry, Yasouj University, Yasouj, Iran

³ Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

⁴ Research Center for Non-communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran

10.22092/ari.2024.366824.3298

Abstract

To evaluate the effect of Metformin, Hypericum perforatum L., and Trigonella gracum seeds alone and combined with chitosan nanoparticles on streptozotocin-induced diabetes in rats (to compare the effects). In this study, 96 adult male Wistar rats were used, which were divided into 12 groups: Control, diabetic, diabetic mellitus receiving buffers, pure chitosan nanoparticles, Metformin at a dose of 250 mg/kg, hydroalcoholic extracts of Hypericum perforatum L. flower at a dose of 200 mg/kg and Trigonella gracum seed at a dose of 100 mg/kg alone and in combination, nano-extract of Hypericum perforatum L., nano-extract of Trigonella gracum seed, alone and in variety. The healthy group received flower extracts of Hypericum perforatum L. and Trigonella gracum seeds at 300 mg/kg. The biochemical test parameters of major liver enzymes, blood glucose¸ weight, malondialdehyde (MDA), plasma iron reducing capacity (FRAP) and antioxidant enzyme superoxide dismutase (SOD), and histopathological changes in liver tissue were determined. The diabetic groups treated with metformin and chitosan nanoparticles containing two extracts alone and in combination, significantly improved rat weight, liver enzyme index of alkaline phosphatase (ALP), MDA, and antioxidant enzyme SOD (P value ≤ 0.05). Chitosan nanoparticles containing the selected extracts combined affected glucose levels, showing more significant improvement than the diabetic groups treated with the extracts alone (P value ≤ 0.05). The livers of diabetic rats treated with an extract of Trigonella gracum seeds alone and with chitosan nanoparticles and with a combination of two parts of Trigonella gracum seeds and Hypericum perforatum L. at a dose of 300 mg/kg alone and in combination with chitosan nanoparticles showed significant improvement in histopathological changes compared with diabetic rats. It seems that the hydroalcoholic extracts of Hypericum perforatum L. and Trigonella gracum seeds, combined with chitosan nanoparticles, are good candidates for further evaluation as effective factors for future control of diabetes.

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