Rabbit corneal wound treatment using small intestinal submucosa (SIS) and platelet rich plasma (PRP) scaffold

Hosseinbabaei, Mohammad; Jahandideh, Alireza; Fattahian, Hamidreza; Mortazavi, Pejman

doi:10.22092/ari.2024.367133.3350

Rabbit corneal wound treatment using small intestinal submucosa (SIS) and platelet rich plasma (PRP) scaffold

Articles in Press

Document Type : Original Articles

Authors

¹ Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.

² Department of Pathology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

10.22092/ari.2024.367133.3350

Abstract

Traumatic corneal wounds trigger numerous inflammatory reactions. This severe inflammation can lead to fibrosis or scarring on the cornea's surface by inhibiting the growth of the natural epithelium. In this study, the healing effects of two simultaneous treatments of small intestine submucosal graft (SIS) and platelet-rich plasma (PRP) in rabbit corneal wound healing were investigated. Twenty white New Zealand rabbits weighing 2.5 to 3 kg, clinically healthy, and with no history of eye disease were selected and divided into four groups (N = 5) and subjected to a wound induction test by crescent knife. Following wound formation, the studied groups included control (absence of corneal wound covering with only physiological serum), PRP+SIS, SIS, and PRP in the form of 1 cc subconjunctival drops of PRP every 12 hours. In groups with SIS, the dressing was placed on the wound with a circumferential suture. With clinical eye examination and fluorescein staining, the wounds were examined in terms of size, infection, turbidity, and edema. 21 days after the operation, half of the animals from each group were killed, and their corneas were evaluated by histopathology. On the 21st day of the study, the PRP+SIS group had the lowest amount of corneal opacity. In the histopathological evaluation, the calculation of the number of rows of epithelium was not significant. The corneas of the PRP and SIS + PRP groups, as well as the SIS group, exhibited significantly less vascularization compared to the control group. The order of stromal collagens was significant in both the SIS group with SIS + PRP and the control group with SIS + PRP. The amount of edema between the control group and the SIS + PRP and PRP groups was significant. The level of inflammation was significantly lower only between the control and SIS+PRP groups had significantly lower levels of inflammation. SIS and PRP alone cannot have the simultaneous use effect that we saw in the SIS and PRP groups. As a result, using the SIS + PRP method in such corneal wounds may be an effective method with less vascularization and inflammation.

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