1
Faculty of Medical Sciences, Tarbiat Modares University
2
Department of Parasitology and Medical Entomology, Faculty of Medical Sciences, Tarbiat Modares University
10.32592/ARI.2025.80.2.579
Abstract
Ilam province is one of the most important centers of Zoonotic Cutaneous Leishmaniasis (ZCL) disease in the west of Iran. This research was conducted to investigate the infectivity of Phlebotomus spp. with Leishmania major in Mehran city of Ilam province, Iran. This study was carried out in the two seasons of the peak of mosquito activity, i.e. summer and autumn of 2019. The sticky papers method was used to collect sandflies. By installing 400 sticky paper traps, 2860 sandflies (950 females and 1910 males) were collected during these two seasons. The female Phlebotomus genus and species were identified using Iranian standard identification key. Then, Leishmania DNA was extracted from the body of the female Phlebotomus using the phenol-chloroform method and amplified by PCR of the ITS1 gene. Then, the genome sequence was compared with the sequence of other samples in the Genbank using bioinformatics software. Finally, based on the phylogenetic tree, the species of the samples of this study was determined. In addition, the parasite species was also determined by using HaeIII restriction enzyme. Among the 617 Phlebotomus female samples collected, 34 phlebotomus female samples were found to be infected with the Leishmania parasite. Of which 32(5.18%) of Ph. papatasi and 2(0.32%) of Ph. sergenti were found to be infected. The results of RFLP method and sequencing indicated that these mosquitoes were infected with only L. major. Based on our results, ZCL type of leishmaniasis is prevalent in Mehran city. It is necessary to pay more attention to the results of this study by health officials of the province.
Ashford R. The leishmaniases as emerging and reemerging zoonoses. Inter J Parasito. 2000; 30(12-13):1269-81.
Lainson R, Shaw JJ. Evolution, classification and geographical distribution. In The Leishmaniases in Biology and Medicine: Volume I Biology and Epidemiology, 1987; vol I.Peters W, Killick-Kendrick R, eds. London: Academic Press Inc, 1–12
Bates P. Axenic culture of Leishmania amastigotes. Parasitol Today. 1993; 9(4):143-6.
Asgari Nezhad H, Borhani M, Norouzi M, Merzaie M. Cutaneous Leishmaniasis in school children in a border area at south-west of Iran. Sci Parasitol. 13(4): 153–158.
Yazdanpanah HA, Rostamianpur M . Analysis of Spatial Distribution of Leishmaniasis and its Relationship with Climatic Parameters (Case Study: Ilam Province). Bull Env Pharmacol Life Sci. 2013; 2(12): 80–86.
Roghani A, Yasemi M, Jalilian M, Abdi J, Rezayee K. Epidemiology of cutaneous leishmaniasis in Ilam. Pajoohesh dar Pezeshki. 2012; 36(5): 50–53 (in Persian).
Gholami Parizad E, Maleki Ravasan N, Gholami Parizad E, Karimian F, Karimian B. Frequency and molecular identification of leishmania parasites in smears prepared from skin lesions of patients referred to health centers of Ilam province by digestion of the rDNA-ITS1 gene. Pathobiol Res (Modares J Med Sci). 2015;18(3):75-85 (in Persian).
Kassiri H, Sharifinia N, Jalilian M, Shemshad K. Epidemiological aspects of cutaneous leishmaniasis in Ilam province, west of Iran (2000–2007). Asian Pac J Trop Dis. 2012; 2:S382-S6.
Kermanjani A, Akhlaghi L, Oormazdi H, Hadighi R. Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran. J Parasit Dis. 2017;41(1):175-9.
Theodor O, Mesghali A. On the phlebotominae of Iran. J Med Entomol. 1964; 1(3):285-300.
Rassi Y, Hanafi Bojd AA. Sandflies, leishmaniasis vectors: morphology, biology, ecology, methods of conducting laboratory and field studies, including an illustrated key to sandflies of Iran. Tehran: book, 2006; No Avaran Eelm Publication (in Persian).
Nezhad HA, Mirzaie M, Sharifi I, Zarean M, Norouzi M. The prevalence of cutaneous leishmaniasis in school children in southwestern Iran, 2009. Comp Clin Pathol 2012; 21(5): 1065–9.
Haddad MHF, Ghasemi E, Maraghi S, Tavala M. Identification of leishmania species isolated from human cutaneous leishmaniasis in Mehran, western Iran using nested PCR. Iran J Parasitol 2016; 11(1): 65–72.
Saberi R, Moin-Vaziri V, Hajjaran H, Niyyati M, Taghipour N, Kheirandish F, Abadi A. Identification of Leishmania species using N-acetylglucosamine-1-phosphate transferase gene in a zoonotic cutaneous leishmaniasis focus of Iran. J Vector Borne Dis 2018; 55: 14–19
Gramiccia M. Recent advances in leishmaniosis in pet animals: epidemiology, diagnostics and anti-vectorial prophylaxis. Vet Parasitol. 2011;181(1):23-30.
Dalimi, A. and Gordani, T. (2025). Leishmania infection in Phlebotomus species in Mehran city, Ilam province, Iran, Iran. Archives of Razi Institute, 80(2), 579-585. doi: 10.32592/ARI.2025.80.2.579
MLA
Dalimi, A. , and Gordani, T. . "Leishmania infection in Phlebotomus species in Mehran city, Ilam province, Iran, Iran", Archives of Razi Institute, 80, 2, 2025, 579-585. doi: 10.32592/ARI.2025.80.2.579
HARVARD
Dalimi, A., Gordani, T. (2025). 'Leishmania infection in Phlebotomus species in Mehran city, Ilam province, Iran, Iran', Archives of Razi Institute, 80(2), pp. 579-585. doi: 10.32592/ARI.2025.80.2.579
CHICAGO
A. Dalimi and T. Gordani, "Leishmania infection in Phlebotomus species in Mehran city, Ilam province, Iran, Iran," Archives of Razi Institute, 80 2 (2025): 579-585, doi: 10.32592/ARI.2025.80.2.579
VANCOUVER
Dalimi, A., Gordani, T. Leishmania infection in Phlebotomus species in Mehran city, Ilam province, Iran, Iran. Archives of Razi Institute, 2025; 80(2): 579-585. doi: 10.32592/ARI.2025.80.2.579