Evaluation of apoptosis and caspase-3 activity in EL4 cell line lymphoma using Moringa oleifera plant extract

Shekarabi, Delaram; Safi, Shahabeddin; Mortazavi, Pejman

doi:10.22092/ari.2024.365162.3047

Evaluation of apoptosis and caspase-3 activity in EL4 cell line lymphoma using Moringa oleifera plant extract

Articles in Press

Document Type : Original Articles

Authors

¹ Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Department of pathobiology, faculty of specialized veterinary sciences, science and research branch, islamic azad university

³ Department of Pathobiology Faculty of Specialized Veterinary Sciences Science and Research Branch Islamic Azad University Tehran, IRAN

10.22092/ari.2024.365162.3047

Abstract

Lymphoproliferative disorders are a group of disorders that involve lymphocytes. These disorders are generally classified into three groups: lymphoma, leukemia, and plasma cell tumors. Lymphomas are among the most common types of malignant tumors in veterinary medicine. They have a high prevalence rate in dogs and cats suffering from diseases such as FeLV. Due to the side effects of chemotherapy drugs, researchers have been exploring the use of plants with medicinal properties. M. oleifera, also known as the miracle tree, is a plant that contains anti-cancer compounds in all its parts, including the leaves, roots, and stems. It has been identified as having high antioxidant potential, making it a valuable plant for promoting health and preventing diseases. M. oleifera is a well-known plant of the Moringaceae family, and its therapeutic potential has been fully determined due to its high antioxidant power Recent studies on Moringa oleifera have shown many anti-inflammatory and anti-cancer properties. The present study aimed to investigate the toxicity effect of moringa extract on the EL4 lymphoma cell line. In the present study, EL4 cells were exposed to different concentrations of M. oleifera extract for 24, 48, and 72 hours. The survival rates of the different groups were first evaluated using trypan blue and MTT assay and then by real-time PCR. The data was analyzed using SPSS software and the one-way ANOVA test. The treatment of the cell line with M. oleifera powder extract at all concentrations inhibited cancer cell growth. The best result was observed at 10 µg/ml after 48 hours, and the IC 50 was higher than 50%. The study concludes that M. oleifera extract inhibits the growth of EL4 cells.

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