Tumor-Associated Tissue Eosinophilia in Oral Squamous Cell Carcinoma: Implications for Histopathologic Grading

Document Type : Original Articles

Authors

1 Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran

2 Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.

3 Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran USERN Office, Qazvin University of Medical Sciences, Qazvin, Iran Network of Interdisciplinarity in Neonates and Infants (NINI), Universal Scientific

4 Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University Of Medical Sciences, Qazvin, Iran

5 Qazvin University of Medical Sciences,Iran

6 Tehran University of Medical Sciences, Iran

10.32592/ARI.2024.79.6.1345

Abstract

Tumor-associated tissue eosinophilia (TATE) has been associated with various tumors, including Oral squamous cell carcinoma, but its role has not been precisely elucidated. This research aimed to investigate the presence of tumor-associated tissue eosinophilia in oral squamous cell carcinomas (OSCCs) and its correlation with histopathologic grading. 70 OSCC tissue samples collected between 2016 and 2020 were examined. The samples comprised 60 previously diagnosed cases of OSCC, classified as well-differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC), and poorly differentiated squamous cell carcinoma (PDSCC). Two observers assessed TATE using Sirius red stain, with the average eosinophil count evaluated in 10 fields under the ×40 objective lens. Statistical analysis involved the Student t-test, One-way ANOVA, and chi-square test. The study encompassed 70 OSCC samples and corresponding healthy tissue, with OSCC predominantly found in the tongue, representing 61.4% of cases. The total eosinophil count per high-power field (HPF10) was significantly higher in OSCC compared to healthy tissue. Moreover, the mean TATE score was notably elevated in OSCC tissue. One-way ANOVA revealed a lack yet statistically significant association between different OSCC grades and eosinophil counts. However, the chi-square test did not indicate a significant association between eosinophil count and gender or age group. This study underscores that cancerous tissues exhibit a heightened eosinophil count compared to healthy tissues. Nonetheless, the variability in eosinophil counts across distinct OSCC grades remains ambiguous. Further investigation is recommended to delve into the infiltration of eosinophils in solid tumors and their potential role in predicting malignancies, particularly in OSCC.

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References

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Archives of Razi Institute
Volume 79, Issue 6
November and December 2024
Pages 1345-1351

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