Inflamatory Markers in Cows Affected by Subclinical Mastitis due to E.coli and Staphylococcus Infections

Document Type : Original Articles

Authors

1 Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran.

2 Internal Medicine and Clinical Pathology Department, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

10.32592/ARI.2024.79.6.1319

Abstract

Investigating the concentrations of Serum Amyloid A (SAA), IL-6, and IL-8 in the serum during episodes of clinical and subclinical mastitis holds significant value. This study aimed to evaluate the diagnostic value of SAA, IL-6, and IL-8 in the early detection of subclinical mastitis in cows infected by Escherichia coli (E.coli) and staphylococcus infections. This cross-sectional analytical study, conducted in 2023 in Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran, evaluated inflammatory markers in 79 dairy cows with clinical and subclinical mastitis. The cows divided into three groups: healthy cows, cows with subclinical mastitis, and cows with clinical mastitis. These groups were then evaluated for Serum Amyloid A (SAA), IL-6, and IL-8. The diagnostic value of the inflammatory markers was determined by calculating the areas under the curves (AUCs) of the ROC curves. Generally, among the patients with a positive culture test (57%), 19% were found to be infected with E. coli, 22.8% with Streptococcus uberis, and 15.2% (12 cases) with Staphylococcus aureus. A strong correlation was observed between the mean SCC and the values of IL-6 (P<0.005), IL-8 (P<0.005), and SAA (P<0.005). Additionally, SAA showed a strong correlation with IL-8 (P<0.005). The value of IL-6 was moderately correlated with both IL-8 (P<0.005) and SAA (P<0.005). The sensitivity and specificity of SCC (0.98), SAA (0.90), IL-6 (0.95), and IL-8 (0.87) were high for diagnosing mastitis in cows. The present study revealed that mastitis in dairy cows is associated with an increase in inflammatory cytokines such as amyloid A, IL-6, and IL-8. The study suggests that variations in these biomarkers could be utilized for disease diagnosis.

Keywords

Main Subjects

References

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Archives of Razi Institute
Volume 79, Issue 6
November and December 2024
Pages 1319-1328

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