Prevalence of biofilm and efflux pump genes expression by PCR and antibiotic resistance pattern in Pseudomonas aeruginosa

Document Type : Original Articles

Authors

1 SabaBiomedicals Science-Based Company, Tehran, Iran

2 Department of Biology, Faculty of Basic Science, Semnan University, Semnan, Iran

3 Department of physiology, school of Medicine Iran University of Medical Science, Tehran, Iran

4 Department of Biology, Faculty of Sciences, Islamic Azad University of Shiraz,Shiraz,Iran

5 Department of Biology, College of Basic Sciences, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

6 Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

10.32592/ARI.2024.79.6.1281

Abstract

Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections. P. aeruginosa is a multidrug-resistant (MDR) bacteria that is thought to be a major cause of nosocomial infections because of its plasmid-borne and intrinsic resistance to several drugs. This study looked at the possibility of biofilm formation, the distribution of the pslD, pelF, and algD genes, as well as the expression of the MexAB-OprM efflux pump genes. It also looked at the pattern of antibiotic resistance in multi-drug resistant P. aeruginosa isolates that were taken from various clinical samples. 76 P. aeruginosa strains had been collected for this investigation from various clinical specimens. Using the disk agar diffusion method, the isolates' susceptibility to antibiotics was assessed. Lastly, multi-drug resistance (MDR) is based on the resistance pattern. These isolates were assessed for the presence of three key biofilm genes and antimicrobial resistance patterns against ten standard antibiotic disks. Version 25 of the SPSS statistical software was used to analyze the data. Examination of the isolates showed that the most antibiotic sensitivity is related to polymyxin, piperacillin, and ciprofloxacin. Also, the prevalence of biofilm-producing genes pslD, pelF, and algD genes was assessed as 68.4%, 80.3%, and 69.7% respectively. The prevalence of MexAB-OprM efflux genes in the examined isolates, mexA, mexB, and oprM genes were 89.5%, 90.8%, and 90.8%, respectively. The majority of the isolates in this investigation had efflux pump genes, according to the findings. Additionally, a strong correlation was discovered between a few efflux genes and biofilm, or the antibiotics tetracycline, meropenem, amikacin, and polymyxin B.

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Main Subjects

References

  1. Driscoll JA, Brody SL, Kollef MH. The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa Drugs. 2007; 67:351-68.
  2. Nicastri E, Petrosillo N, Martini L, Larosa M, Gesu GP, Ippolito G. Prevalence of nosocomial infections in 15 Italian hospitals: first point prevalance study for the INF-NOS project. Infection. 2003; 1; 31:10-5.
  3. Gill JS, Arora S, Khanna SP, Kumar KH. Prevalence of multidrug-resistant, extensively drug-resistant, and pan drug-resistant Pseudomonas aeruginosa from a tertiary level Intensive Care Unit. J Global Infect Dis 2016; 8:155-9.
  4. Bahador N, Shoja S, Faridi F, Dozandeh-Mobarrez B, Qeshmi FI, Javadpour S, et al. Molecular detection of virulence factors and biofilm formation in Pseudomonas aeruginosa obtained from different clinical specimens in Bandar Abbas. Iran J Microbiol 2019; 11(1):25-30
  5. Vickers ML, Dulhunty JM, Ballard E, Chapman P, Muller M, Roberts JA, et al. Risk factors for multidrug‐resistant G ram‐negative infection in burn patients. ANZ Journal of Surgery 2018; 88(5): 480-485.
  6. Ciofu O, Tolker-Nielsen T. Tolerance and resistance of Pseudomonas aeruginosa biofilms to antimicrobial agents—how aeruginosa can escape antibiotics. Frontiers in microbiology. 2019;3; 10:913.
  7. Abdulhaq N, Nawaz Z, Zahoor MA, Siddique AB. Association of biofilm formation with multi drug resistance in clinical isolates of Pseudomonas aeruginosa. EXCLI journal. 2020; 19:201.
  8. Thi MT, Wibowo D, Rehm BH. Pseudomonas aeruginosa biofilms. International journal of molecular sciences. 2020;17;21(22):8671.
  9. Borchaloee AS, Hashemi PB. Antibiotic Resistance and Biofilm Formation of Pseudomonas aeruginosa, a therapeutic challenge: Narrative Review. J Med Case Rep Case Series. 2023;4(07).
  10. Rojo-Bezares B, Cavalié L, Dubois D, Oswald E,Torres C, Sáenz YJJomm. Characterization of carbapenem resistance mechanisms and integrons in Pseudomonas aeruginosa strains from blood samples in a French hospital. J Med Microbiol.2016;65(4):311-9.
  11. Dreier J, Ruggerone PJFim. Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa. Front Microbiol. 2015;6:660.
  12. Tian Z-X, Yi X-X, Cho A, O'Gara F, Wang Y-PJP. CpxR activates MexAB-OprM efflux pump expression and enhances antibiotic resistance in both laboratory and clinical nalB-type isolates of Pseudomonas aeruginosa. PLOS. 2016;12(10):e1005932.
  13. Banar M, Emaneini M, Satarzadeh M, Abdellahi N, Beigverdi R, Leeuwen WBv, et al. Evaluation of mannosidase and trypsin enzymes effects on biofilm production of Pseudomonas aeruginosa isolated from burn wound infections. PloS one. 2016;11(10):e0164622.
  14. Mitov I, Strateva T, Markova B. Prevalence of virulence genes among Bulgarian nosocomial and cystic fibrosis isolated of Pseudomonas aeruginosa. Brazilian Journal of Microbiology. 2010; 41: 588-595
  15. Mesaros N, Glupczynski Y, Avrain L, Caceres NE, Tulkens PM, Van Bambeke F. A combined phenotypic and genotypic method for the detection of Mex efflux pumps in Pseudomonas aeruginosa. J Antimicrob Chemother 2007; 59: 378-386.
  16. Yoneda K, Chikumi H, Murata T, Gotoh N, Yamamoto H, Fujiwara H, et al. Measurement of Pseudomonas aeruginosa multidrug efflux p umps by quantitative real-time polymerase chain reaction. FEMS Microbiol Lett 2005; 243: 125-131.
  17. Savli H, Karadenizli A, Kolayli F, Gundes S, Ozbek U, Vahaboglu H. Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR. J Med Microbiol 2003; 52: 403-408.
  18. Haji Hossein Tabrizi A, Habibi M, Foroohi F,Mohammadian T, Asadi KaramMR.Adib Hajbaghe M, Dianati M. Evaluation of the effect of IDR-1018 antimicrobial peptide and chitosan nanoparticles on imipenem susceptible and resistant Pseudomonas aeruginosa isolated from patients with urinary tract infections from ICU. Journal of Torbat Heydariyeh University of Medical Sciences. 2022;10(2):1-12.
  19. Plant AJ, Dunn A, Porter RJJEroa-it. Ceftolozane- tazobactam resistance induced in vivo during the treatment of MDR Pseudomonas aeruginosa Expert Rev Anti Infect Ther 2018;16(5):367-8.
  20. Beig M, Arabestani M R. Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of Pseudomonas aeruginosa isolated from Intensive Care Unit. Iran J Med Microbiol. 2019; 13 (2) :142-150.
  21. Siasi E, Rafiei Tabatabaii R, MoslehiMehr F. Isolation of bla_vim gene in Antibiotic resistant Pseudomonas aeruginosa from hospitals. New Cellularand Molecular Biotechnology Journal. 2018;8(29):97-106.
  22. Aminizadeh Z, Kashi MS. Prevalence of multi-drug resistance and pan drug resistance among multiple gram-negative species: experience in one teaching hospital, Tehran, Iran. Int Res J Microbiol 2011; 2:90-5.
  23. Fazeli H, Havaei SA, Solgi H, Shokri D, Motallebirad T. Pattern of Antibiotic Resistance in Pesudomonas aeruginosa Isolated from Intensive Care Unit, Isfahan, Iran. J Isfahan Med Sch 2013; 31(232): 433-8.
  24. Rajabi H, Salimizand H, Khodabandehloo M, Fayyazi A, Ramazanzadeh R. Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes involved in biofilm formation in clinical Pseudomonas aeruginosa BioMed research international. 2022 May 24;2022.
  25. Abdulhaq N, Nawaz Z, Zahoor MA, Siddique AB. Association of biofilm formation with multi drug resistance in clinical isolates of Pseudomonas aeruginosa. EXCLI journal. 2020; 19:201.
Archives of Razi Institute
Volume 79, Issue 6
November and December 2024
Pages 1281-1286

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