In-vitro Pharmacological activity of Endophyte Aspergillus austwickii isolated from leaves of Premna serratifolia

Document Type : Original Articles

Authors

1 P G Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad 580003, Karnataka, India.

2 Division of Pre-clinical Research and Drug Development, Cytxon Biosolutions Pvt Ltd, Hubli-580031, Karnataka, India.

3 Department of Bioinformatics and Biotechnology, Karnataka State Akkamahadevi Women’s University, Vijayapura, Karnataka, India.

4 PG Department of Biotechnology and Microbiology, Karnatak University, Dharwad-580003

10.32592/ARI.2024.79.5.1023

Abstract

Diabetes mellitus is one of the most widely occurring non communicable diseases spreading rapidly worldwide that occurs as a result of non-regulation of glucose in the blood stream or because of improper functioning of the enzymes α- amylase and α-glucosidase. Endophytes are the microorganisms that reside symbiotically inside the living tissues of plants. Endophytic fungi have the ability to synthesize various important bioactive metabolites. The current study was aimed to explore the less reported endophytic fungus Aspergillus austwickii isolated from ethnobotanical medicinal plant Premna serratifolia L. The methanolic extract of the endophytic fungus was subjected to activities like in vitro antioxidant assays, anti-inflammatory assay, antidiabetic assay in addition to exploring their total phenolic and total flavonoid content along with the phytochemicals present. The results revealed that Aspergillus austwickii exhibited phytoconstituents like alkaloids, phenols, flavonoids, tannins and carbohydrates. The total phenolic content and total flavonoid content of the fungus was found to be 22.048 µg GAE/g and 18.828 µg GAE/g. The crude extract showed 46.20+0.53% of antioxidant activity with an IC50 value of 128.69 µg/mL for radical scavenging by DPPH. It also exhibited 71.86+0.34% of anti-inflammatory activity by protein denaturation assay. Notably, it revealed, antidiabetic activity for both alpha amylase and alpha glucosidase with the percentage of inhibition of 68.22+0.17% and 73.72+0.18% with an IC50 value of 178.10 µg/mL and 166.16µg/mL respectively. The current study shows that the methanolic extract of Aspergillus austwickii possesses considerable antioxidant, anti-inflammatory and antidiabetic capabilities. The findings of the current research can be explored for the future research to find out new set of natural drugs which can be used against several disorders.

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References

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Archives of Razi Institute
Volume 79, Issue 5
September and October 2024
Pages 1023-1030

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