Effects of Thymus daenensis essential oil-loaded chitosan nanoparticles on BCR1 gene expression in Candida parapsilosis

Document Type : Original Articles

Authors

Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

10.32592/ARI.2024.79.5.973

Abstract

Background & Objective: Candida parapsilosis is a non-albicans species with high prevalence and potential for nosocomial infections. The BCR1 gene is involved in the regulation of virulence factors in this species. This study aimed to evaluate the effects of Thymus daenensis essential oil encapsulated in chitosan nanoparticles (TDNs) on the expression of the BCR1 gene in C. parapsilosis isolates from animal and human sources.
Materials & Methods: Sixty C. parapsilosis isolates (30 from human and 30 from veterinary sources) were screened for the presence of the BCR1 gene by PCR. The TDNs were synthesized and characterized by various techniques. The isolates carrying the BCR1 gene were treated with TDNs to determine the minimum inhibitory concentration (MIC). The expression of the BCR1 gene after treatment with sub-MIC concentrations of TDNs was measured by real-time PCR and compared with the control group. The results were statistically analyzed.
Results: Five out of 60 isolates (8.33%) were positive for BCR1 gene. The physical properties of TDNs showed that they had a spherical shape, an average size of 92.3 nm, a polydispersity index of 0.129± 0.03, a zeta potential of +48.3 mV, and an encapsulation efficiency of 88.6 ± 0.2%. The MIC range for TDNs in these isolates was 0.032-1 μg/ml. The treatment with TDNs significantly reduced the expression of the BCR1 gene in all five isolates compared with the control group (p=0.012).
Conclusion: TDN has a suitable potential for inhibiting the expression of the BCR1 gene, which is associated with virulence in C. parapsilosis. This may enhance the antifungal activity of TDN and reduce the risk of nosocomial infections caused by this species.

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References

  1. Selim AO, Abdel Salam MM, Abdallah Hassan RN, Mustafa GE, Abdelrahman Mahdy Z. The Effect of Some Nano Plant Extract on Bacteria Producing Biogenic Amines Isolated From Minced Meat. Iranian Journal of Veterinary Medicine. 2024;18(4):501-516.
  2. Branco J, Miranda IM, Rodrigues AG. Candida parapsilosis virulence and antifungal resistance mechanisms: A comprehensive review of key determinants. Journal of Fungi. 2023;9(1):80.
  3. Hosseinabadi E, Garoussi MT, Khosravi AR, Gharagozloo F, Toosi BK, Moosakhani F. Prevalence of Prototheca and Fungal Contamination of Bulk Milk Tank of Industrial Dairy Cattle Herds in Iran. Iranian Journal of Veterinary Medicine. 2022;16(2):155-165.
  4. Barchiesi F, Orsetti E, Osimani P, Catassi C, Santelli F, Manso E. Factors related to outcome of bloodstream infections due to Candida parapsilosis complex. BMC infectious diseases. 2016;16(1):1-7.
  5. Daneshnia F, de Almeida Júnior JN, Ilkit M, Lombardi L, Perry AM, Gao M, et al. Worldwide emergence of fluconazole-resistant Candida parapsilosis: current framework and future research roadmap. The Lancet Microbe. 2023;4(6):e470-e480.
  6. Luzala MM, Muanga CK, Kyana J, Safari JB, Zola EN, Mbusa GV, et al. A critical review of the antimicrobial and antibiofilm activities of green-synthesized plant-based metallic nanoparticles. Nanomaterials. 2022;12(11):1841.
  7. Branco JAV. Unveiling the antifungal resistance mechanisms of the emergent fungal pathogen Candida parapsilosis. 2023.
  8. Ebrahimpour M, Akhlaghi M, Hemati M, Ghazanfary S, Shahriary S, Ghalekohneh SJ, et al. In vitro evaluation and comparison of anticancer, antimicrobial, and antifungal properties of thyme niosomes containing essential oil. Nanomedicine Journal. 2022;9(4):307-318.
  9. Ahmadi H, Sheikh-Assadi M, Fatahi R, Zamani Z, Shokrpour M. Optimizing an efficient ensemble approach for high-quality de novo transcriptome assembly of Thymus daenensis. Scientific Reports. 2023;13(1):12415.
  10. Hamouda T, Hayes MM, Cao Z, Tonda R, Johnson K, Wright DC, et al. A novel surfactant nanoemulsion with broad-spectrum sporicidal activity against Bacillus species. Journal of Infectious Diseases. 1999;180(6):1939-49.
  11. Granata G, Stracquadanio S, Leonardi M, Napoli E, Malandrino G, Cafiso V, et al. Oregano and thyme essential oils encapsulated in chitosan nanoparticles as effective antimicrobial agents against foodborne pathogens. Molecules. 2021;26(13):4055.
  12. Sotelo-Boyás M, Correa-Pacheco Z, Bautista-Baños S, y Gómez YG. Release study and inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules against foodborne bacteria. International Journal of Biological Macromolecules. 2017;103:409-14.
  13. Poon Y, Hui M. Inhibitory effect of lactobacilli supernatants on biofilm and filamentation of Candida albicans, Candida tropicalis, and Candida parapsilosis. Frontiers in Microbiology. 2023;14:1105949.
  14. Poon Y, Hui M. Inhibitory effect of lactobacilli supernatants on biofilm and filamentation of C. albicans, C. tropicalis, and C. parapsilosis. bioRxiv. 2023;14:1105949.
  15. Modiri M, Hashemi SJ, Ghazvini RD, Khodavaisy S, Ahmadi A, Ghaffari M, et al. Antifungal susceptibility pattern and biofilm-related genes expression in planktonic and biofilm cells of Candida parapsilosis species complex. Current Medical Mycology. 2019;5(4):35.
  16. Rafique S, Murtaza MA, Hafiz I, Ameer K, Qayyum MMN, Yaqub S, et al. Investigation of the antimicrobial, antioxidant, hemolytic, and thrombolytic activities of Camellia sinensis, Thymus vulgaris, and Zanthoxylum armatum ethanolic and methanolic extracts. Food Science & Nutrition. 2023;11(10):6303-6311.
  17. Jain N, Choudhary P. Phytochemistry, Traditional Uses and Pharmacological Aspect of Thymus vulgaris: A Review. Indian Journal of Pharmaceutical Sciences. 2022;84(6):1369-79.
  18. Tardugno R, Serio A, Purgatorio C, Savini V, Paparella A, Benvenuti S. Thymus vulgaris L. essential oils from Emilia Romagna Apennines (Italy): Phytochemical composition and antimicrobial activity on food-borne pathogens. Natural Product Research. 2022;36(3):837-42.
  19. Aksit H, Bayar Y, Simsek S, Ulutas Y. Chemical composition and antifungal activities of the essential oils of Thymus species (Thymus pectinatus, Thymus convolutus, Thymus vulgaris) against plant pathogens. Journal of Essential Oil Bearing Plants. 2022;25(1):200-7.
  20. Anvar S, Mehdipoor H, Sohrabi Haghdoost N. Evaluation the antifungal effect of thymol and its effect on NOR1 gene expression in Aspergillus flavus isolates. Veterinary Research & Biological Products. 2022;35(2):69-78.
  21. Moazeni M, Davari A, Shabanzadeh S, Akhtari J, Saeedi M, Mortyeza-Semnani K, et al. In vitro antifungal activity of Thymus vulgaris essential oil nanoemulsion. Journal of Herbal Medicine. 2021;28:100452.
  22. Jeddi M, Fikri-Benbrahim K, El Hachlafi N, Benkhaira N, Aboussemdai A, Ouaritini ZB. Chemical Composition of Thymus vulgaris, Origanum compactum and Vetiveria zizanoides Essential oils and their Antibacterial and Antioxidant Activities. Tropical Journal of Natural Product Research. 2023;7(1):2244-50.
  23. Erfaninejad M, Aboualigalehdari E, Fatahinia M. Effect of Peganum harmala Extract on Biofilm and Involved Gene Expression in Biofilm Production of Candida albicans. Jundishapur Journal of Microbiology. 2023;16(2):e132692.
Archives of Razi Institute
Volume 79, Issue 5
September and October 2024
Pages 973-980

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