Molecular Analysis of Nucleocapsid Gene and 3' Untranslated Regions of an Infectious Bronchitis Virus Isolate Originated from Broilers in Maragheh

Document Type : Original Articles

Authors

1 Department of cellular and molecular biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran

2 department of Biology, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran

10.32592/ARI.2024.79.4.789

Abstract

Avian infectious bronchitis virus, has become one of the most problematic causes of economic losses in poultry farms. To effectively control the virus, monitoring and surveillance of circulating strains of virus in poultry farms is unavoidable.
Internal organ samples of broilers with clinical signs of infectious bronchitis and two samples of the commonly used vaccine strains (4/91 and H120) in Iranian poultry flocks were used in amplification of a 1.8 kbp fragment including nucleocapsid (N) gene and 3′ untranslated region (UTR) using reverse transcription and polymerase chain reaction (RT-PCR) method. The amplified fragments were digested using restriction endonuclease enzyme, AluI. Then sequence similarity of the field isolate (Ma1/16 ) with previous isolates and reference strains of IBV was investigated. Also, phylogenetic relationship of Ma1/16 with other regions viruses were determined based on the sequence of two 600 bp partial sequences of N gene using Mega7 software.
Seven strains of IBV were classified in two groups based on restriction fragment length polymorphism (RFLP) patterns of the N-3´UTR fragment; all of five field isolates and vaccine strain 4/91 were clustered together. Ma1/16 had the most similarity with two other Iranian IBV isolates, Ur1/09 and MNS-7861-1 (91.7 % and 90 %, respectively) based on the 600 N sequence of 5´ end of the isolate. Nucleotide sequence of the 600 N of 3´ end of the amplified fragment in Ma1/16 isolate had the most similarity to the BJ strain (86.4%).
Nucleocapsid gene could be an appropriate candidate in vaccine design strategies to IBV effective control. In addition, monitoring of circulating strains of IBV based on N-3´UTR could be a helpful method in successfully controlling of IB disease in Iran.

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References

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Archives of Razi Institute
Volume 79, Issue 4
July and August 2024
Pages 789-798

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