Prevalence of Trichomonas gallinae in Birds of Iran and its Pathological Findings: A Review study

Document Type : Mini Review

Authors

1 Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

10.32592/ARI.2024.79.3.445

Abstract

Trichomonas gallinae is a flagellated protozoan parasite that affects numerous avian species worldwide, causing a range of diseases collectively termed trichomonosis. This review study aimed to present a comprehensive analysis of the prevalence of T. gallinae in birds of Iran, along with an exploration of its associated pathological findings. Through an extensive search of published studies, scientific databases, and relevant literature, we identified several studies conducted in Iran that focused on the prevalence of T. gallinae in different bird populations as well as their pathological effect. These studies utilized a variety of techniques, including direct microscopic examination, culture methods, and molecular tools to detect and identify the presence of this parasite. This review study indicates that T. gallinae infections are prevalent among various bird species in Iran, including Domestic pigeons, Zebra Finches and Turkey. The prevalence rates reported showed significant differences among various regions and bird species, ranging from as low as 2.5% in zebra finches to as high as 70% in pigeons. The clinical signs of infected birds varied from no clinical signs to visible yellowish-white masses of caseous necrotic material, hyperplasia of mucous cells and significant congestion of lung. These findings underline the importance of T. gallinae as a significant pathogen affecting avian populations in Iran. Using at least two techniques such as culture or PCR in addition to direct smear is recommended for better diagnosis of infection. The study highlights the need for further epidemiological studies to better understand the risk factors associated with T. gallinae transmission and to develop effective prevention and control strategies.

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Main Subjects

Full Text

  1. Introduction

Iran is a vast country located in the Middle East, with a diverse landscape and an incredible biodiversity. The climate of this country is influenced by its geographical location and is predominantly arid or semiarid, with hot and dry summers and cold winters. This country, with its diverse habitats ranging from wetlands and forests to mountains and deserts, provides a hospitable environment for various bird species (1). Trichomonas gallinae is an avian protozoan parasite that commonly infects birds, particularly pigeons and doves (2-4). This protozoan parasite is responsible for causing a contagious disease known as trichomoniasis or "canker" in birds (5). These birds are frequently identified as natural carriers of T. gallinae, serving as a reservoir for the parasite. Several raptor species, including hawks, eagles, and falcons, have been reported to be affected by this parasite (6-8). Waterbirds, such as ducks, swans, and geese, have also been found to carry T. gallinae. Additionally, passerine birds, such as finches, sparrows, and songbirds, have been documented as hosts for T. gallinae (9, 10). T. gallinae thrives in the upper gastrointestinal tract, including the crop and throat of infected birds, leading to various symptoms that can range from mild discomfort to severe illness and even death. Understanding the biology, transmission, and impact of T. gallinae is crucial for effective disease prevention and management in bird populations. Numerous bird species have been reported to be infected with T. gallinae, making it a significant concern for avian health and conservation efforts worldwide (11). The present review of studies conducted between 2008 and 2023 aimed to assess the presence of T. gallinae in Iran.

 

  1. Prevalence of Trichomonas galinae infections in avian in Iran

In 2008, a study was conducted in Iran to expand the understanding of T. gallinae in pigeons (12). Following that, numerous studies investigated the presence of T. gallinae infection in birds from various areas of Iran (Table 1). From 2008 until now, domestic pigeons, zebra finches, and turkeys from Iran were examined using methods to detect trophozoites and cysts. The findings of these studies are presented in Table 1. The comparison of prevalence rates of trichomoniasis between turkey, Zebra Finches, and pigeons in Iran indicates that pigeons play a more significant role in sustaining the cycle in the mentioned area than other bird species. Thease finding was incontinent with the same previous study (13). According to Table 1, the occurrence of trichomoniasis was more common in regions with dry weather compared to areas with high levels of precipitation in Iran. This finding reinforces the hypothesis that dry weather and limited rainfall have been proposed as potential factors contributing to the rise of this disease (14). The present review study, which included research conducted over the last 15 years, performed an analysis of the presence of T. gallinae infection in more than 29% of Iranian territory (9 out of 31 counties). Infected avians were found in all studied areas during this period. As the number of examined animals and the detection methods varied drastically among the different studies, the comparative contribution of different host species to the parasite's maintenance is difficult to assess.

 

  1. Pathological lesion of Trichomonas gallinae

In some studies conducted on the progression of trichomoniasis in pigeons from Iran, no significant lesions and evident clinical symptoms caused by the disease were identified (12, 15). In another study conducted in the same region, researchers observed visible yellowish-white masses of caseous necrotic material in the mouths, throats, food storage organs, and stomachs of infected pigeons and turkeys. Additionally, there was also hyperplasia of mucous cells in the epithelia of the trachea. The lungs exhibited significant congestion (16). In contrast to previous studies conducted in other parts of the world (12), the invasion of the mucosal lesion to the sinuses of the skull and penetrating eyes and brain was not reported.

 

  1. Conclusion

The present review showed that the T. gallinae infection rate is relatively high among the Iranian avian population. The T. gallinae infection is still significantly present in Iran, infecting several wild and domestic avian species with different prevalence rates over the years, thus maintaining the focus on the parasites. Using at least two techniques, such as culture or PCR, in addition to direct smear is recommended for better diagnosis of infection and understanding the actual prevalence of T. gallinae.

References

  1. Amiri M, Eslamian S. Investigation of climate change in Iran Journal of Environmental Science and Technology. 2010;3(4):208-16.
  2. Nasrabadi NT, Mirghasemi N, Bakhtiari N, Zarrinkoub N, Mirghasemi N. Comparison of anti-parasitic effects of garden thyme (Thymus vulgaris) extract and metronidazole on Trichomonas gallinae. Research in Pharmaceutical Sciences. 2012;7(5):788.
  3. Marx M, Reiner G, Willems H, Rocha G, Hillerich K, Masello JF, et al. High prevalence of Trichomonas gallinae in wild columbids across western and southern Europe. Parasites & Vectors. 2017;10:1-11.
  4. Shemshadi B, Ranjbar-bahadori S, Delfan-abazari M. Prevalence and intensity of parasitic infection in domestic ducks (Anas platyrhynchas) in Gilan Province, Northern Iran. Comparative Clinical Pathology. 2017;26:165-7.
  5. Seddiek SA, El-Shorbagy MM, Khater HF, Ali AM. The antitrichomonal efficacy of garlic and metronidazole against Trichomonas gallinae infecting domestic pigeons. Parasitology Research. 2014;113:1319-29.
  6. Boal CW, Mannan RW, Hudelson KS. Trichomoniasis in Cooper's hawks from Arizona. Journal of Wildlife Diseases. 1998;34(3):590-3.
  7. Martínez-Herrero M, Sansano-Maestre J, Azami-Conesa I, González-González F, Suárez Regalado L, Garijo-Toledo M, et al. Sequence subtyping of Trichomonas gallinae from Bonelli’s eagle (Aquila fasciata) during four years (2014–2017) reveals that MLS type is associated with lesions. Avian Pathology. 2021;50(4):339-49.
  8. Taiefi Nasrabadi N, Baygan A, Yousefi M. Comparison of the effect of garlic (Allium sativum) ethanol extract with metronidazole on Trichomonas gallinae. Journal of Veterinary Laboratory Research. 2015;6(2):117-25.
  9. Chi JF, Lawson B, Durrant C, Beckmann K, John S, Alrefaei AF, et al. The finch epidemic strain of Trichomonas gallinae is predominant in British non-passerines. Parasitology. 2013;140(10):1234-45.
  10. Chen D-q, Luo X-y, Li Q-q, Pan J-c, Zhang H, Gu Y-y, et al. Molecular prevalence of Tetratrichomonas gallinarum and Trichomonas gallinae in three domestic free-range poultry breeds in Anhui Province, China. Parasitology Research. 2022;121(10):2841-8.
  11. Sansano-Maestre J, Garijo-Toledo MM, Gómez-Muñoz MT. Prevalence and genotyping of Trichomonas gallinae in pigeons and birds of prey. Avian Pathology. 2009;38(3):201-7.
  12. Pirali-Kheirabadi K, Shakhse-Niaie M, Hosseini N. Epidemiological aspects of pigeon trichomonosis in center of Iran. Res J Parasitol. 2008;3:20-4.
  13. Quillfeldt P, Schumm YR, Marek C, Mader V, Fischer D, Marx M. Prevalence and genotyping of Trichomonas infections in wild birds in central Germany. PloS one. 2018;13(8):e0200798.
  14. Forzan MJ, Vanderstichel R, Melekhovets YF, McBurney S. Trichomoniasis in finches from the Canadian Maritime provinces—an emerging disease. The Canadian Veterinary Journal. 2010;51(4):391.
  15. Nematollahi A, Ebrahimi M, Ahmadi A, Himan M. Prevalence of Haemoproteus columbae and Trichomonas gallinae in pigeons (Columba domestica) in Isfahan, Iran. Journal of Parasitic Diseases. 2012;36:141-2.
  16. Borji H, Razmi G, Movassaghi A, Moghaddas E, Azad M. Prevalence and pathological lesion of Trichomonas gallinae in pigeons of Iran. Journal of Parasitic Diseases. 2011;35:186-9.
  17. Adinehbeigi K, Ebrahimi M, Soltani Eini M, Samiei A. Prevalence of Haemoproteus columbae (apicomplexa: haemoproteidae) and Trichomonas gallinae (metamonada: trichomonadidae) infections among pigeons (Columba livia) in west Azerbaijan province, Iran. Archives of Razi Institute. 2018;73(2):147-52.
  18. Ayati R, Nasr-Abadi NT, Momeni Z, Shojaei S. Prevalence of Trichomonas gallinae with molecular characterisation and phylogenetic analysis in domestic pigeons using ITS1-5.8 s rRNA-ITS2 gene in Alborz province, Iran. Bulgarian Journal of Veterinary Medicine. 2023;26(1):65-72.
  19. Madani SA, Arabkhazaeli F, Eram N. Trichomonosis in a flock of zebra finches (Taeniopygia guttata). Journal of Exotic Pet Medicine. 2015;24(4):430-4.
  20. Mirzaei M, Ghashghaei O, Khedri J. First report of an outbreak trichomoniasis in turkey in Sistan, Iran. Journal of Parasitic Diseases. 2016;40:61-4.
  21. Radfar M, Fathi S, Asl E, Dehaghi M, Seghinsara H. A survey of parasites of domestic pigeons (Columba livia domestica) in South Khorasan, Iran. Veterinary Research (Pakistan). 2011;4(1):18-23.
  22. Soltanieini M, Ghorashi A, Tavassoli M. Molecular detection of Trichomonas gallinae by using of 5.8S rRNA gene by PCR reaction in Columbidae birds. Iranian Journal of Veterinary Clinical Sciences. 2016;10(2).
Archives of Razi Institute
Volume 79, Issue 3
May and June 2024
Pages 445-448

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