Newcastle disease vaccination program in broilers using a Apathogenic heat-resistant vaccine

Document Type : Original Articles

Authors

Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

10.22092/ari.2023.363053.2800

Abstract

The Newcastle disease (ND) is among the most common and deadly poultry diseases worldwide. Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. The Newcastle Disease V4 Heat-Resistant (NDV4HR) vaccine is an Apathogenic heat-resistant live vaccine that can induce immunity in chickens. A total of 60 one-day-old Arain local hybrid broilers divided into three groups of 20. Group A had the usual vaccination program in broiler flocks (7 Days old: B1 type by eye drop+ ND Killed vaccine by IM; 18 and 28 days old: Lasota strain orally), group B did not receive any vaccine, and Group C received orally the NDV4HR vaccine six times from the first day to the 35th day of rearing. All groups were nasally challenged with acute Newcastle virus (genotype VIId) on the 35th rearing day. Regarding HI serum antibody titer of the birds after the challenge, group B had the highest (7.12 ± 1.05), and Group C (6.37 ± 1.31) had a higher titer than Group A (6 ± 1.22). After the challenge with the Newcastle virus, the necropsy findings and clinical symptoms of the disease were almost similar in groups A and C. Group B showed the most signs with higher casualties than other groups. Regarding weight gain, Group C had the most weight at the end of the study (2204 ± 106). On the second day after the challenge, groups A and B had virus shedding through the trachea and cloaca; however, group B shed the virus only through the trachea. Furthermore, on the 7th day after the challenge, group A shed the virus through the cloaca, and group B through the trachea and the cloaca. At the same time, there was no virus shedding in group C. This study showed that V4HR vaccine provokes an antibody response that protects the birds against virulent virus challenge.

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Archives of Razi Institute

Articles in Press, Accepted Manuscript
Available Online from 22 November 2023

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