Isolation, characterization, and antimicrobial resistance profiles of Campylobacter jejuni and Campylobacter coli from raw meat of large livestock in Iran

Document Type : Original Articles

Authors

1 Department of Food Hygiene and Public Health, Shahrekord Branch, Islamic Azad University

2 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

3 Research Center of Nutrition and Organic Products, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

10.32592/ARI.2024.79.1.41

Abstract

Campylobacter spp. genera are one of the most common causes of microbial enteritis worldwide. This research aims to discover how common Campylobacter organisms are in raw meat from large livestock in Iran and their antibiotic susceptibility profiles. Several 550 fresh ready-to-eat meat samples were gathered from the slaughterhouse, butcher shops, and restaurants in the research region, including cattle (n = 138), goat (n = 102), camel (n = 56), and sheep (n = 254) meats samples. Using routine bacteriological procedures and PCR, Campylobacter spp. was isolated and identified. PCR was used to perform genotype pattering in the identification of virulence genes. The disc diffusion technique was used to determine antibiotic susceptibility. The two Campylobacter spp. were found in 84 (15.27%) of the 550 meat test samples. Cattle and camel samples had the greatest (52.38 %) and lowest (3.57%) frequency of Campylobacter spp., respectively. There were significant variations in the prevalence of Campylobacter spp. in cattle compared to others (P < 0.01; 2 = 43.04 or OR = 7.68, CI = 3.40–17.30). C. jejuni and C. coli amounted to 82.14 % (69 samples) of the Campylobacter spp. they were isolated from the raw meat. C. jejuni was found in 39.28% of the samples (33 samples), whereas C. coli was found in 42.85% (36 samples). Other Campylobacter spp. made up 17.85 % (15 samples). The most prevalent genotypes pattering observed in C. jejuni bacteria collected from several sorts of large livestock samples were ciaB (100%) and flaA (100%), and virbll (7.69 %) were the C. jejuni strains found with the least incidence in various large livestock specimens. ciaB (100 %), flaA (100 %), were the most common genotypes found in C. coli bacteria. The C. coli isolates dnaJ (0%), wlaN (0%), virbll (0%), and ceuE (0%) discovered with the least frequency in a range of large livestock samples. Campylobacter spp. isolated from various sample types and sources were 100% sensitive to aphA-3-1 and GM10. Resistance to E15 (76.93 %), cmeB (69.24 %), aadE1 (69.24 %), CIP5 (69.24 %), and AM10 (69.24 %) was found in the isolates.

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References

References
1. Gholipour-Shoshod, A., Rahimi, S., Zahraei Salehi,
T., Karimi Torshizi, M. A., Behnamifar, A.,
Ebrahimi, T., Valizadeh Lakeh, M., Ganjpoor, F.
Evaluating the Competitiveness of Medicinal Plants
with Antibiotics to Control Salmonella Enterica
Serovar Typhimurium in Broiler Chickens. Iranian
Journal of Veterinary Medicine, 2023; 17(2): 155-
166. doi: 10.32598/ijvm.17.2.1005233.
2. Mehrabi, F., Rassouli, M., Emadi Chashmi, S. H.
Molecular Detection of Toxoplasma gondii in
Chicken Meats and Eggs in Semnan City, Iran.
Iranian Journal of Veterinary Medicine, 2023;
17(2): 167-172. doi: 10.32598/ijvm.17.2.1005238.
3. Heredia N, García S. Animals as sources of foodborne pathogens: A review. Animal nutrition.
2018;4(3):250-5.
4. Pijnacker R, Friesema IH, Mughini Gras L,
Lagerweij GR, Van Pelt W, Franz E. Disease
burden of food-related pathogens in the
Netherlands, 2018.
5. Wysok B, Sołtysiuk M, Stenzel T. Wildlife
Waterfowl as a Source of Pathogenic
Campylobacter Strains. Pathogens. 2022
Feb;11(2):113.
6. Aminharati F, Ehrampoush MH, Dallal MM, Yaseri
M, Tafti AA, Rajabi Z. Citrobacter freundii
foodborne disease outbreaks related to
environmental conditions in Yazd Province, Iran.
Iranian journal of public health. 2019;48(6):1099.
7. Edslev SM, Clausen ML, Agner T, Stegger M,
Andersen PS. Genomic analysis reveals different
mechanisms of fusidic acid resistance in
Staphylococcus aureus from Danish atopic
dermatitis patients. Journal of Antimicrobial
Chemotherapy. 2018 ;73(4):856-61.
8. Mousavinafchi, S. B., Rahimi, E., & Shakerian, A.
(2022). Campylobacter spp. isolated from poultry in
Iran: Antibiotic resistance profiles, virulence genes,
and molecular mechanisms. Food science &
nutrition, 11(2), 1142–1153.
https://doi.org/10.1002/fsn3.3152
9. Gharaghie P. Tohid, and Seyed Ataollah Sadat
Shandiz.". The Inhibitory Effects of Silver
Nanoparticles on Bap Gene Expression in
Antibiotic-Resistant Acientobacter bumanni Isolates
using Real-Time PCR." scientific journal of ilam
university of medical sciences. 2018;26:175-85.
10. Ayukekbong JA, Ntemgwa M, Atabe AN. The
threat of antimicrobial resistance in developing
countries: causes and control strategies.
Antimicrobial Resistance & Infection Control.
2017;6(1):1-8.
11. Berhanu L, Bedru H, Gume B, Tolosa T, Kassa T,
Getaneh A, Mereta ST. Occurrence, Risk Factors,
Rahimi et al / Archives of Razi Institute, Vol. 79, No. 1 (2024) 41-54 53
and Antimicrobial Susceptibility Test of
Thermophilic Campylobacter Species of Bovine
Carcass at Municipal Abattoir and Butcher Shops of
Jimma Town, Southwest Ethiopia. Infection and
Drug Resistance. 2021; 14:3753.
12. Franco J, Bénejat L, Ducournau A, Mégraud F,
Lehours P, Bessède E. Evaluation of
CAMPYLOBACTER QUIK CHEK™ rapid
membrane enzyme immunoassay to detect
Campylobacter spp. antigen in stool samples. Gut
Pathogens. 2021;13(1):1-6.
13. Dabiri H, Aghamohammad S, Goudarzi H, Noori
M, Hedayati MA, Ghoreyshiamiri SM. Prevalence
and antibiotic susceptibility of Campylobacter
species isolated from chicken and beef meat.
International Journal of Enteric Pathogens.
2016;2(2):6-17087.
14. Rahimi E, Tajbakhsh E. Prevalence of
Campylobacter species in poultry meat in the
Esfahan city, Iran. Bulg. J. Vet. Med.
2008;11(4):257-62.
15. Habib I, Sampers I, Uyttendaele M, Berkvens D, De
Zutter L. Belgium-wide survey of Campylobacter
spp. contamination in chicken meat preparations:
baseline data. In13th Conference on Food
Microbiology 2008.
16. Marinou I, Bersimis S, Ioannidis A, Nicolaou C,
Mitrousia-Ziouva A, Legakis NJ, Chatzipanagiotou
S. Identification and antimicrobial resistance of
Campylobacter species isolated from animal
sources. Frontiers in microbiology. 2012;3:58.
17. Rahimi E, Kazemeini HR, Safaei S, Allahbakhshi
K, Momeni M, Riahi M. Detection and
identification of Campylobacter spp. from retail raw
chicken, turkey, sheep and goat meat in Ahvaz,
Iran. African journal of microbiology research.
2010;4(15):1620-3.
18. Pezzotti G, Serafin A, Luzzi I, Mioni R, Milan M,
Perin R. Occurrence and resistance to antibiotics of
Campylobacter jejuni and Campylobacter coli in
animals and meat in northeastern Italy. International
journal of food microbiology. 2003;82(3):281-7.
19. Salihu MD, Junaidu AU, Oboegbulem SI, Egwu
GO. Prevalence and biotypes of Campylobacter
species isolated from sheep in Sokoto State,
Nigeria. International Journal of Animal and
Veterinary Advances. 2009 Jul 1;1(1):6-9.
20. Taremi M, Dallal MM, Gachkar L, MoezArdalan S,
Zolfagharian K, Zali MR. Prevalence and
antimicrobial resistance of Campylobacter isolated
from retail raw chicken and beef meat, Tehran, Iran.
International Journal of Food Microbiology.
2006;108(3):401-3.
21. E Nonga H, Sells P, Karimuribo ED. Occurrences
of thermophilic Campylobacter in cattle slaughtered
at Morogoro municipal abattoir, Tanzania. Tropical
Animal Health and Production. 2010;42(1):73-8.
22. Vanderlinde PB, Shay B, Murray J. Microbiological
quality of Australian beef carcass meat and frozen
bulk packed beef. Journal of Food Protection.
1998;61(4):437-43.
23. Thépault A, Poezevara T, Quesne S, Rose V,
Chemaly M, Rivoal K. Prevalence of thermophilic
Campylobacter in cattle production at
slaughterhouse level in France and link between C.
jejuni bovine strains and Campylobacteriosis.
Frontiers in Microbiology. 2018; 9:471.
24. Chen X, Naren GW, Wu CM, Wang Y, Dai L, Xia
LN, Luo PJ, Zhang Q, Shen JZ. Prevalence and
antimicrobial resistance of Campylobacter isolates
in broilers from China. Veterinary microbiology.
2010;144(1-2):133-9.
25. Rahimi E. Occurrence and resistance to antibiotics
of Campylobacter spp. in retail raw sheep and goat
meat in Shahr-e Kord, Iran. Global Veterinaria.
2010;4(5):504-9.
26. Mpalang RK, Boreux R, Melin P, Daube G, De Mol
P. Prevalence of Campylobacter among goats and
retail goat meat in Congo. The Journal of Infection
in Developing Countries. 2014;8(02):168-75.
27. Rahimi E, Kazemeini HR, Safaei S, Allahbakhshi
K, Momeni M, Riahi M. Detection and
identification of Campylobacter spp. from retail raw
chicken, turkey, sheep and goat meat in Ahvaz,
Iran. African journal of microbiology research.
2010 Aug 4;4(15):1620-3.
28. Beiranvand S, Piri‐Gharaghie T, Dehganzad B,
Khedmati F, Jalali F, AsadAlizadeh M, Momtaz H.
Novel NAD‐independent Avibacterium
paragallinarum: Isolation, characterization and
molecular identification in Iran. Veterinary
Medicine and Science. 2022 Feb 19.
29. Church DL, Cerutti L, Gürtler A, Griener T,
Zelazny A, Emler S. Performance and application
of 16S rRNA gene cycle sequencing for routine
54 Rahimi et al / Archives of Razi Institute, Vol. 79, No. 1 (2024) 41-54
identification of bacteria in the clinical
microbiology laboratory. Clinical Microbiology
Reviews. 2020;33(4):e00053-19.
30. Hagos Y, Gugsa G, Awol N, Ahmed M, Tsegaye Y,
Abebe N, Bsrat A. Isolation, identification, and
antimicrobial susceptibility pattern of
Campylobacter jejuni and Campylobacter coli from
cattle, goat, and chicken meats in Mekelle, Ethiopia.
PloS one. 2021;16(2):e0246755.
31. Faris G. Identification of Campylobacter species
and their antibiotic resistance patterns from raw
bovine meat in addis ababa, Ethiopia. International
Journal of Multimedia Information Retrieval.
2015;4(1):1-5.
32. Paintsil EK, Ofori LA, Adobea S, Akenten CW,
Phillips RO, Maiga-Ascofare O, Lamshöft M, May
J, Obiri Danso K, Krumkamp R, Dekker D.
Prevalence and Antibiotic Resistance in
Campylobacter spp. Isolated from Humans and
Food-Producing Animals in West Africa: A
Systematic Review and Meta-Analysis. Pathogens.
2022;11(2):140.
33. Aleksić E, Miljković-Selimović B, Tambur Z,
Aleksić N, Biočanin V, Avramov S. Resistance to
Antibiotics in Thermophilic Campylobacters.
Frontiers in Medicine. 2021;8.
34. Rossi DA, Dumont CF, de Souza Santos AC, de
Lourdes Vaz ME, Prado RR, Monteiro GP, da Silva
Melo CB, Stamoulis VJ, Dos Santos JP, de Melo
RT. Antibiotic Resistance in the Alternative
Lifestyles of Campylobacter jejuni. Frontiers in
Cellular and Infection Microbiology. 2021;11.
35. Hagos Y, Gugsa G, Awol N, Ahmed M, Tsegaye Y,
Abebe N, Bsrat A. Isolation, identification, and
antimicrobial susceptibility pattern of
Campylobacter jejuni and Campylobacter coli from
cattle, goat, and chicken meats in Mekelle, Ethiopia.
PloS one. 2021;16(2):e0246755.
36. Emes D, Naylor N, Waage J, Knight G. Quantifying
the Relationship between Antibiotic Use in FoodProducing Animals and Antibiotic Resistance in
Humans. Antibiotics. 2022;11(1):66.
37. Toledo Z, Simaluiza RJ, Fernández H. Occurrence
and antimicrobial resistance of Campylobacter
jejuni and Campylobacter coli isolated from
domestic animals from Southern Ecuador. Ciência
Rural. 2018;48.
38. Ayukekbong JA, Ntemgwa M, Atabe AN. The
threat of antimicrobial resistance in developing
countries: causes and control strategies.
Antimicrobial Resistance & Infection Control.
2017;6(1):1-8.
39. Manyi-Loh C, Mamphweli S, Meyer E, Okoh A.
Antibiotic use in agriculture and its consequential
resistance in environmental sources: potential public
health implications. Molecules. 2018;23(4):795.
40. Oh JY, Kwon YK, Wei B, Jang HK, Lim SK, Kim
CH, Jung SC, Kang MS. Epidemiological
relationships of Campylobacter jejuni strains
isolated from humans and chickens in South Korea.
Journal of Microbiology. 2017;55(1):13-20.
41. Otigbu AC, Clarke AM, Fri J, Akanbi EO, Njom
HA. Antibiotic sensitivity profiling and virulence
potential of Campylobacter jejuni isolates from
estuarine water in the Eastern Cape Province, South
Africa. International Journal of Environmental
Research and Public Health. 2018;15(5):925.
42. Pillay S, Amoako DG, Abia AL, Somboro AM,
Shobo CO, Perrett K, Bester LA, Essack SY.
Characterisation of Campylobacter spp. isolated
from poultry in KwaZulu-Natal, South Africa.
Antibiotics. 2020;9(2):42.
43. González-Hein G, Huaracán B, García P, Figueroa
G. Prevalence of virulence genes in strains of
Campylobacter jejuni isolated from human, bovine
and broiler. Brazilian Journal of Microbiology.
2013;44(4):1223-9
Archives of Razi Institute
Volume 79, Issue 1
January and February 2024
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