Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography
1
Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt.
2
Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt.
10.32598/ARI.81.1.3445
Abstract
Introduction: Hydatid disease (HD) or hydatidosis is a major public health problem that is spreading all over the world. The diagnosis of hydatid disease is considered to be challenging; however, serological diagnosis provides an early diagnostic approach, and confirmation of the existence of the hydatid cyst microscopically is still necessary. Enzyme-linked immunosorbent assay (ELISA) is one of the best diagnostic methods. Materials & Methods: Sandwich ELISA, nano-sandwich ELISA, and the immunochromatographic test (ICT) were conducted utilizing purified hydatid antigen for the diagnosis of human hydatidosis. The analyzed sera comprised samples from patients infected with hydatidosis (n=50), patients infected with other helminthic parasites (20, 20, and 10 with Schistosoma mansoni, Fasciola hepatica, and Ancylostoma duodenale, respectively), and 50 healthy negative control subjects. Results: The findings indicated that the sandwich ELISA detected positive results in 39 out of 50 hydatid-infected samples, yielding an assay sensitivity of 78%. Seven out of 50 samples from parasite-infected groups yielded positive results, resulting in 93% specificity. In the nano-sandwich ELISA, 42 of 50 hydatid-infected samples yielded positive results, which produced an assay sensitivity of 84%. seven out of 50 samples from other parasite-infected groups had positive results, giving 93% specificity. Using the ICT, 36 out of 50 hydatid-infected samples yielded positive results, which gave an assay sensitivity of 72%. sixteen out of 50 from other parasite-infected groups had positive results, giving 80% specificity. Diagnostic specificity reached 93%, 93%, and 80% by using sandwich ELISA, nano-sandwich ELISA, and ICT techniques, respectively. Conclusion: The use of nano-sandwich ELISA is one of the best methods for the diagnosis of human hydatidosis.
Ahmed, M. , Mohamed, S. H. , Shalash, I. R. , Zalat, R. S. M. , Aboueldahab, M. M. and Nigm, A. M. (2026). Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography. Archives of Razi Institute, 81(1), 89-98. doi: 10.32598/ARI.81.1.3445
MLA
Ahmed, M. , , Mohamed, S. H. , , Shalash, I. R. , , Zalat, R. S. M. , , Aboueldahab, M. M. , and Nigm, A. M. . "Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography", Archives of Razi Institute, 81, 1, 2026, 89-98. doi: 10.32598/ARI.81.1.3445
HARVARD
Ahmed, M., Mohamed, S. H., Shalash, I. R., Zalat, R. S. M., Aboueldahab, M. M., Nigm, A. M. (2026). 'Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography', Archives of Razi Institute, 81(1), pp. 89-98. doi: 10.32598/ARI.81.1.3445
CHICAGO
M. Ahmed , S. H. Mohamed , I. R. Shalash , R. S. M. Zalat , M. M. Aboueldahab and A. M. Nigm, "Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography," Archives of Razi Institute, 81 1 (2026): 89-98, doi: 10.32598/ARI.81.1.3445
VANCOUVER
Ahmed, M., Mohamed, S. H., Shalash, I. R., Zalat, R. S. M., Aboueldahab, M. M., Nigm, A. M. Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography. Archives of Razi Institute, 2026; 81(1): 89-98. doi: 10.32598/ARI.81.1.3445
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