The analysis of Norduz Sheep Mandible with DFA and PCA

Document Type : Original Articles

Authors

1 Department of Anatomy, Faculty of Veterinary Medicine, Kafkas University, Kars-36100, Turkey

2 Department of Surgery, Faculty of Veterinary Medicine, Kafkas University, Kars-36100, Turkey

10.32592/ARI.2025.80.1.139

Abstract

Norduz sheep are known as a variety of Akkaraman sheep bred in the Norduz region, which is located within the borders of the Gürpınar district of Van province. Norduz sheep are bred only in the Norduz region, adapt to the harsh climate of the Eastern Anatolia region, and show unique performance indicators that distinguish this breed from other breeds.
In this study, the mandible of Norduz sheep was examined by geometric morphometric method and analyzed. Principal components in multidimensional data sets were determined by Principal Component Analysis. The differences between the samples were determined by Discriminant Function Analysis. For this purpose, study materials were obtained from slaughterhouses in Van. A total of 20 mandibles (10F/10M) were used since analyzes were made in terms of gender. The mandibles were first dissected from the skull. Later, the overlying muscles were dissected. After boiling, they were kept in hydrogen peroxide for 20-30 minutes and the mandibles were bleached. After drying, the mandibles were photographed from the same distance (20 cm). The left lateral sides of the mandibles were used for photographing. Statistical and formal analyzes of these variances were also performed. As a result of the analyzes made, 16 variances were obtained within the scope of principal component analyses. The first three of the variances obtained explained 58,647 of the shape differences. According to the discriminant function analysis, which gave the best results for gender discrimination, the results were very good and individuals were completely separated from each other. In line with these analyzes, information was obtained about the anatomical features and adaptations of the Norduz sheep mandible, and it became an exemplary study in this field.

Keywords

Main Subjects


  1. Koyuncu, M. Sheep Breeding (in Turkish). Bursa, Turkey. Dora Publishing Distribution Ltd. Şti. 1st edn. 2019;42.
  2. Mis, L., Mert, H., Comba, A., Comba, B., Söğütlü, İD., Irak, K., Mert, N. (2018). Some mineral substance, oxidative stress and total antioxidant levels in Norduz and Morkaraman sheep. Van Vet J, 29 (3), 131-134.
  3. Delibaş, V., Soygüder, Z., Çakmak, G., Gündüz, MS. Morphological Examination of Tongue Papillae in Norduz Sheep: A Scanning Electron Microscopic Study. Van Veterinary Journal, 2023;34 (1) , 75-80.
  4. Dalga, S., Aksu, Sİ., Aslan, K., Deprem, T., Uğran, R. (2021). Morphological, Morphometrical and Histological Structure of the Interdigital Gland in Norduz Sheep. Kafkas Univ Vet Fak Derg, 2021, 27 (6), 749-754.
  5. Dalga, S., Aydın, U., Çal, T. (2022). Topographic, Morphological and Morphometric Investigation of Mandible in Norduz Sheep. Türk Doğa ve Fen Dergisi, 2022, 11 (3) , 129-133. DOI: 10.46810/tdfd.1100636
  6. Ozcan, S., Aksoy, G., Kurtul, I., Aslan, K., Ozudogru, Z. (2010). A comparative morphometric study on the skull of the Tuj and Morkaraman sheep. Kafkas Universitesi Veteriner Fakultesi Dergisi 2010; (16):111–114. https:// doi. org/ 10. 9775/ KVFD. 2009. 518
  7. Karimi, I., Onar, V., Pazvant, G., Hadipour, M., Mazaheri, Y. (2011). The cranial morphometric and morphologic characteristics of Mehraban sheep in Western Iran. Glob Vet, 2011, 6:111–117
  8. Mohamed, R., Driscoll, M., Mootoo, N. Clinical anatomy of the skull of the Barbados Black Belly sheep in Trinidad. Int J Curr Res Med Sci. 2016;2:8–19
  9. Dalga, S., Aslan, K., Kırbaş, G. (2017). Hemşin Koyunu mandibulası üzerinde morfometrik bir çalışma. Atatürk Üniv Vet Bil Derg, 2017; 12(1): 22-7.
  10. Dalga, S., Aslan, K., Akbulut, Y. A morphometric study on the skull of the adult Hemshin sheep. Van Vet J. 2018;29:125–129
  11. Dalga, S., & Aslan, K. Topographic and morphometric study on mental foramen in Hemshin sheep for local anesthesia. MJAVL, 2020, 10:93–97
  12. Wehausen, JD., & Ramey, RR. Cranial morphometric and evolutionary relationships in the northern range of Ovis canadensis. J Mammal, 2000, 81:145–161.
  13. Dalga, S., & Aslan, K. Morphological and Osteometric Analysis of the Skull in Abaza goats (Capra aegagrus), Dicle Üniv Vet Fak Derg. 2021;14(1):35-38.
  14. Dalga, S. Topographic and morphometric study of the mental foramina of Abaza goats with its clinical implication for regional anesthesia Folia Morphol, 2020; 3(79): pp. 576–579.
  15. Jashari, T., Duro, S., Gündemir, O., Tomasz, S., Vlatko, I., Dashmir, M., Om Prakash, C. (2022). Morphology, morphometry and some aspects of clinical anatomy in the skull and mandible of Sharri sheep. Biologia, 2022;77, 423–433.
  16. Gündemir, O., Duro, S., Jashari, T., Kahvecioğlu, O., Demircioğlu, İ., Mehmeti, H. (2020). A study on morphology and morphometric parameters on skull of the Bardhoka autochthonous sheep breed in Kosovo. Anat HistolEmbryol. 2020;00:1–7.
  17. Yılmaz, B., & Demircioglu, İ. (2020). İvesi Koyunlarda (Ovis aries) Kafatasının Morfometrik Analizi. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 2020; 34:01–06
  18. Özdoğru, Z., İlgün, R., Teke, BE. (2019). Konya Merinosunun Mandibula’sı Üzerine Morfometrik Bir Çalışma. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 5(2): 392-395.
  19. Babaei, M., Yavari, M., Zolhavarieh, S. M., Kalantari Hesari, A. A Case History of Gross and Radiological Observations of Agnathia: Otocephaly in a Mehraban Ewe-lamb. Iranian Journal of Veterinary Medicine, 2023; 17(4): 415-422. doi: 10.32598/ijvm.17.4.1005231
  20. Marzban Abbasabadi, B., Ghojoghi, F., Rahmati, S., Hajian, O. Morphological Variations in the Skulls of Male and Female Persian Fallow Deer (Dama dama mesopotamica). Iranian Journal of Veterinary Medicine, 2021; 15(2): 221-232. doi: 10.22059/ijvm.2020.299875.1005075
  21. Samuel, O., Korzerzer, B., Olopade, J. O., Onwuka, S. K. Acomparative osteometric evaluation of some cranial indices of clinical significance in goats (Capra hircus) from the middlebelt regions of Nigeria. Iranian Journal of Veterinary Medicine, 2013; 7(2): 111-116. doi: 10.22059/ijvm.2013.35315
  22. Klingenberg, CP., & Marugan-Lobon, J. (2013). Evolutionary Covariation in Geometric Morphometric Data: Analyzing İntegration, Modularity, And Allometry İn A Phylogenetic Context. Syst. Biol, 62: 591–610, 2013.Ff
  23. Szara, T., Duro, S., Gündemir, O., Demircioğlu, İ. (2022). Sex Determination in Japanese Quails (Coturnix japonica) Using Geometric Morphometrics of the Skull. Animals, 2022, 12: 302.
  24. Koçak, S., Özaydın, İ., Gündemir, O. (2023). Sağlıklı ve Septik Artritli Buzağılarda Karpal Eklemin Radyometrik ve Geometrik Morfometrik Analizi. Kafkas Üniv, Sağlık Bilimleri Enstitüsü, Doktora Tezi, Kars, 2023, (in Turkish).
  25. Duro, S., Gündemir, O., Sönmez, B., Jashari, T., Szara, T., Pazvant, G., Kambo, A. (2021). A Different Perspective on Sex Dimorphism in the Adult Hermann’s Tortoise: Geometric Morphometry. Zoological Studies, 60 (9): 3-9.
  26. Hadžiomerović, N., Gundemir, O., Tandir, F., Avdić, R., Katica, M. (2023). Geometric and Morphometric Analysis of the Auditory Ossicles in the Red Fox (Vulpes vulpes). Animals 2023, 13, 1230.
  27. Demircioglu, I., Demiraslan, Y., Gurbuz, I., Dayan, MO. (2021). Geometric Morphometric Analysis of Skull and Mandible in Awassi Ewe and Ram. Kafkas Üniv. Vet. Fakültesi Derg.2021, 27, 43–49.
  28. Gürbüz, I., Aytek, AI., Demiraslan, Y.,  Onar, V.,  Özgel, Ö. (2020). Geometric morphometric analysis of cranium of wolf (Canis lupus) and German shepherd dog (Canis lupus familiaris). Kafkas Üniv. Vet. Fakültesi Derg. 2020, 26, 525–532. 
  29. Gündemir, O., Özkan, E., Dayan, MO., Aydogdu, S. (2020). Sexual analysis in turkey (Meleagris gallopavo) neurocranium using geometric morphometric methods.  J. Vet. Anim. Sci. 2020, 44, 681–687.
  30. Parés-Casanova, PM., Tolić, A., Carnicero, R. (2020). Side differences in the skull of sheep: An assessment by geometric morphometrics. Annu Res Rev Biol, 34 (2): 1-7, 2020. DOI: 10.9734/arrb/2019/v34i230147
  31. Parés Casanova, PM. (2015). Geometric morphometrics to the study of skull sexual dimorphism in a local domestic goat breed. J Fisheries Livest Prod, 3:141. DOI: 10.4172/2332-2608.1000141
  32. Fernandez Blanco, MV., Cassini, GH., Bona, P. (2023). A three-dimensional geometric morphometric analysis of the morphological transformation of Caimanlower jaw during post-hatching ontogeny. PeerJ, 11:e15548
  33. Marcus, LF., Hingst-Zaher, E., Zaher, H. (2000). Application of landmark morphometrics to skulls representing the orders of living mammals. Hystrix, 2000, 11, 27-47.
  34. Yalçın, H., Kaya, MA., Arslan, A. (2010). Comparative geometrical morphometries on the mandibles of Anatolian wild sheep (Ovis gmelini anatolica) and Akkaraman sheep (Ovis aries). Kafkas Univ Vet Fak Derg, 16 (1): 55-61, 2010.
  35. Özkan, E., Jashari, T., Gündemir, O., İnce, NG. (2020). Morphometric Analysis of the Mandible of Bardhoka Autochthonous Sheep in Kosovo. Anat Histol Embryol, 2020, (1): 1-5.
  36. Gündemir, O., Pazvant, G., Jashari, T., Dayan, MO. (2020). A morphometric study of metapodium in Bardhoka sheep. Res Agric Vet Sci, 2020, 4 (1): 30-38.
  37. Kahraman, M., Değirmencioğlu, İ., Gülşah, G., Gündemir, O., Özkan, E., Das, A., Yılmaz, B. (2022). Determination of the correlations between the morphological characteristics and metapodial radiometric measurements of Awassi sheep. Eurasian J Vet Sci, 2022, 38 (2): 180-189.