Effect of Different Levels of Metabolizing Energy on Productive Performance of Two Grower Quail Strains

Document Type : Original Articles

Authors

Department of Animal production, College of Agriculture and Forestry, University of Mosul, Mosul, Iraq

Abstract

Two strains of Japanese quail (Coturnix coturnix japonica), including 250 birds of desert and 250 birds of white color, all one day old and unsexed, were divided into five treatment groups with 50 replicates in each group. These treatments included five levels of metabolism energy (ME) levels, including 2700, 2800, 2900, 3000, and 3100 Kcal/Kg diet. The study included one stage from day 1 to day 42 of birds’ age. The results confirmed a statistically significant difference (P≤0.05) caused by ME levels in the body weight, weight gain, feed conversion ratio (feed [g]: weight gain [g]), water consumption, water conversion ratio (ml: weight gain[g]), protein conversion ratio (protein [g]: weight gain [g]), energy conversion ratio (Kcal: weight gain [g]), carcass weight, in addition to albumin and triglyceride. Therefore, the results showed significant effects (P≤0.05) of ME levels and the interaction on feed consumption, protein consumption, edible giblet percentage, tenderness, and juiciness. Significant differences (P≤0.05) were also caused by ME levels in the total cholesterol. In addition, significant differences (P≤0.05) have been found in the interaction on mortality percentage. Net return (Iraqi Dinar/live weight [Kg]) for desert quail was better than that for the white quail (2900 Kcal/Kg diet), and the interaction effect was stronger on the desert strain with 2900 Kcal than the white strain.

Keywords


1. Introduction

In poultry nutrition, energy is divided into gross energy, digestible energy, metabolism energy (ME), and net energy. The ME for feedstuff used in poultry nutrition is the basic energy ( 1 ). The main factor that affects feed intake is ME in the ration. The grower quail stops the intake of the ration when it gets the requirements of ME so that the ration is balanced in all elements and compounds. Some researchers studied the best ME levels in the Japanese quail rations. They found that there were significant differences in body weight when they used different ME levels on the grower of Japanese quail rations, while Hasanien ( 2 ), Muniz, Barreto ( 3 ), as well as Muniz, Barreto ( 4 ), did not find any significant effects on body weight when they used different ME levels on Japanese quail rations. On the other hand, some other researchers studied the effect on Japanese quail strains, such as Bughio, Jatoi ( 5 ), as well as Al-Kafajy, Al-Shuhaib ( 6 ), who found significant differences in body weight when they used different strains of Japanese quail, while Ahmad, Mehmood ( 7 ).

2. Materials and Methods

Two strains of Japanese quail (Coturnix coturnix japonica), including 250 birds of desert and 250 birds of white color, all one day old and unsexed, were divided into five treatment groups, each containing 50 replicates. This treatment included five levels of ME levels, including 2700, 2800, 2900, 3000, and 3100 Kcal/Kg diet. The study included one stage (the growth stage) from day 1 to day 42 of birds’ age. It was completed at the Department of Animal Production, University of Mosul, Mosul, Iraq. Iron cages sized 50×50×50 cm were used to house the birds. The feed contained approximately 24% crude protein. The nutrient requirements of the grower quail and the chemical composition of rations used in this study were formulated according to the National Research Council ( 8 ) tabulated in table 1.

Ingredients Energy Level (Kcal./Kg diet)
T1 T2 T3 T4 T5
2700 2800 2900 3000 3100
Soybean meal 28.40 29.10 30.20 31.40 32.50
Yellow corn 10 16.20 24 34 42
Wheat 46.40 40 30.70 19.30 10
Wheat bran 5.50 3.90 3 2 1
Sunflower oil 0.50 1.60 2.90 4.10 5.30
Protein concentrate 8 8 8 8 8
Limestone 0.50 0.50 0.50 0.50 0.50
Di Calcium phosphate 0.20 0.20 0.20 0.20 0.20
Vit. Min. premix 0.25 0.25 0.25 0.25 0.25
Common salt 0.25 0.25 0.25 0.25 0.25
Total 100 100 100 100 100
Chemical Analysis
ME(kcal/kg diet) 2700 2801 2902 3000 3100
Crude protein % 24.06 24.01 24.02 24.01 24.04
ME / P ratio 112.2 116.7 120.8 124.9 129
Ether extract % 2.76 3.89 5.23 6.50 7.76
crude fiber % 4.45 4.23 4.09 3.95 3.81
Calcium % 0.99 0.99 1 1 1
A. phosphorus % 0.47 0.46 0.46 0.45 0.44
Lysine % 1.32 1.32 1.35 1.36 1.38
Methionine % 0.56 0.56 0.57 0.56 0.56
Linoleic acid % 1.14 1.86 2.69 3.52 4.31
Table 1.Chemical composition of experimental diets in this research (1-42 days)

The water intake and the feed conversion ratio were calculated as stated in Kesab ( 9 ), with the following equations: 1) water intake (ml/bird)=the added water (ml/bird)−the remaining water (ml/bird) and 2) water conversion ratio (ml/gm weight gain)=water intake (ml/bird)/weight gain (gm/bird). At the end of the research (day 42), the birds were slaughtered, and the characteristics of their carcasses were recorded as reported previously ( 9 ). The sensory evaluation (tenderness, juiciness, flavor, and acceptability) of the Japanese quail breast meat was carried out according to Kesab ( 9 ). The economic calculations of the search parameters were calculated on day 42 to produce 1 Kg of live weight, depending on Kesab ( 9 ). The Statistical Analysis Software (SAS, 2002) was used to statistically analyze the study data for the factorial and two-factor experiment. The study followed a complete random design. The difference between the means was tested by Duncan ( 10 ) multi-range test. Finally, the standard error of each transaction was measured.

3. Results and Discussion

The results in table 2 showed significant differences (P≤0.05) caused by the strain, ME levels, and the interaction factor on body weight and weight gain. The strain factor significantly affected the body weight and weight gain of the desert quail. These results agree with the findings of Al-Fleeh ( 11 ). The most significant effects on body weight and weight gain were caused by the T3 (2900 Kcal/Kg diet) ME level. The interaction factor also caused significant differences (P≤0.05) in body weight and weight gain in T3 ME level (the interaction between desert quail and 2900 Kcal ME). However, the three factors did not significantly affect mortality percentages.

Treatments Body weight (g/bird) Weight gain (g/bird) Growth rate (%) Mortality (%)
Strain effect
Desert quail 193.03 ±3.35 A 185.58 ±3.36 A 185.14 ±1.65 0.40 ±0.40
White quail 185.03 ±1.87 B 177.65 ±1.87 B 184.65 ±2.13 0.80 ±0.54
Metabolism energy levels effect (Kcal./Kg) :
2700 186.95 ±1.63 BC 179.56 ±1.63 BC 184.79 ±1.75 0 ±0
2800 185.73 ±2.97 BC 178.31 ±2.98 BC 184.62 ±1.33 0 ±0
2900 198.60 ±6.14 A 191.20 ±6.12 A 185.63 ±2.08 0 ±0
3000 193.33 ±3.70 AB 185.92 ±3.70 AB 185.23 ±0.96 1 ±1
3100 180.53 ±4.12 C 173.11 ±4.18 C 184.20 ±1.82 2 ±1.26
Interaction: Strain × Metabolism energy levels (Kcal./Kg) :
Desert quail 2700 186.48 ±3.02 BC 179.04 ±3 BC 184.65 ±1.27 0 ±0
2800 188.71 ±3.51 BC 181.24 ±3.55 BC 184.77 ±0.90 0 ±0
2900 209.67 ±7.01 A 202.22 ±7.03 A 186.27 ±3.12 0 ±0
3000 197.55 ±3.27 AB 190.13 ±3.31 AB 185.51 ±1.88 2 ±2
3100 182.72 ±8.46 BC 175.27 ±8.48 BC 184.32 ±1.51 0 ±0
White quail 2700 187.42 ±1.99 BC 180.07 ±2.03 BC 184.90 ±2.35 0 ±0
2800 182.75 ±4.79 BC 175.37 ±4.81 BC 184.46 ±2.65 0 ±0
2900 187.52 ±4.10 BC 180.17 ±4.06 BC 184.90 ±2.02 0 ±0
3000 189.11 ±5.59 BC 181.71 ±5.60 BC 184.93 ±1.12 0 ±0
3100 178.33 ±2.90 C 170.94 ±2.90 C 184.08 ±0.87 4 ±4
A,c: Mean in the same column with no common superscripts differ significantly (P<0.05)
Table 2.Effect of treatments and interaction, effect on body weight, weight gain, growth rate, and mortality percentage in the research (1-42 days)

Table 3 showed significant differences (P≤0.05) in feed conversion ratio, water intake, and water conversion ratio caused by the strain factor; however, the difference caused in feed intake was not significant. Significant differences (P≤0.05) were caused by the strain effect on the desert quail in feed conversion ratio and water intake. As for the effect of ME levels, the T1 (2700 Kcal) ME level had the strongest effect on feed intake and water intake, followed by the T2 (2880 Kcal) ME level. On the other hand, T3 (2900 Kcal), followed by T4 (3000 Kcal) ME levels had the strongest effect on the feed conversion ratio. In the interaction, significant differences (P≤0.05) in feed intake were caused by T6 and T7 ME levels, while significant differences in water intake were caused by T7 ME level. The T3 ME level recorded the best feed conversion ratio.

Treatments Feed intake (g/bird) Feed conversion ratio (gm: gm gain) Water intak (ml/bird) Water conversion ratio (ml: gm gain)
Strain effect
Desert quail 688.49 ±10.37 3.71 ±0.11 B 1749.20 ±33.98 B 9.43 ±0.18 B
White quail 714.84 ±13.92 4.02 ±0.09 A 1878.26 ±32.57 A 10.57 ±0.25 A
Metabolism energy levels effect (Kcal./Kg) :
2700 742.56 ±16.29 A 4.14 ±0.12 A 1933.84 ±59.25 A 10.77 ±0.34 A
2800 740.36 ±13.42 A 4.15 ±0.11 A 1945.71 ±45.05 A 10.91 ±0.81 A
2900 669.42 ±19.17 B 3.50 ±0.17 B 1680.80 ±31.14 B 8.79 ±0.30 B
3000 685.33 ±16 B 3.69 ±0.13 B 1770.62 ±21.91 B 9.52 ±0.25 B
3100 670.67 ±15.23 B 3.87 ±0.07 AB 1737.69 ±35.58 B 9.99 ±0.29 AB
Interaction: Strain × Metabolism energy levels (Kcal./Kg) :
Desert quail 2700 722.88 ±13.68 AB 4.04 ±0.22 AB 1870.48 ±115.02 BC 10.45 ±0.41 AB
2800 718.37 ±4.90 AB 3.96 ±0.06 AB 1859.13 ±32.38 C 10.26 ±0.35 AB
2900 666.27 ±25.89 B 3.29 ±0.23 C 1620.65 ±17.22 D 8.01 ±0.16 C
3000 680.08 ±10.52 AB 3.58 ±0.08 BC 1733.33 ±16.93 CD 9.12 ±0.23 BC
3100 654.84 ±7.87 B 3.74 ±0.24 ABC 1662.43 ±3.58 D 9.48 ±0.33 ABC
White quail 2700 762.23 ±21.10 A 4.23 ±0.11 A 1997.20 ±19.36 AB 11.09 ±0.18 A
2800 762.34 ±20.91 A 4.35 ±0.23 A 2032.29 ±40.06 A 11.59 ±0.18 A
2900 672.56 ±20.19 B 3.73 ±0.22 ABC 1740.94 ±30.58 CD 9.66 ±0.18 ABC
3000 690.58 ±29.97 AB 3.80 ±0.27 ABC 1807.90 ±26.90 C 9.95 ±0.18 ABC
3100 686.49 ±29.12 AB 4.02 ±0.23 AB 1812.95 ±25.58 C 10.61 ±0.18 AB
a,c: Mean in the same column with no common superscripts differ significantly (P<0.05)
Table 3.Effect of treatments energy levels and interaction on feed intake, feed conversion ratio, and water intake in the research (1-42 days)

Table 4 shows significant effects (P≤0.05) of the three factors on protein conversion ratio and ME conversion ratio. The strain effect was the most significant on protein conversion ratio and ME conversion ratio in the desert quail. Regarding the ME levels, the strongest effect on protein intake was in T1 and T2, and on protein conversion ratio and ME conversion ratio, it was in T3. The interaction results show the best protein and ME conversion ratios for T3 ME level (desert quail with 2900 Kcal).

Treatments Protein intake (g/bird) Protein conversion ratio (g: g gain) ME intake (Kcal./bird) ME conversion ratio (Kcal. : g gain)
Strain effect
Desert quail 165.43 ±2.81 0.89 ±0.02 B 1993.54 ±26.17 10.74 ±0.29 B
White quail 171.76 ±3.43 0.97 ±0.02 A 2068.99 ±32.45 11.65 ±0.27 A
Metabolism energy levels effect (Kcal./Kg) :
2700 178.66 ±4.24 A 1 ±0.02 A 2004.90 ±47.61 11.17 ±0.33 AB
2800 177.76 ±3.22 A 1 ±0.02 A 2073.73 ±37.13 11.63 ±0.31 A
2900 160.80 ±4.60 B 0.84 ±0.04 B 1942.65 ±55.63 10.16 ±0.51 B
3000 164.55 ±3.84 B 0.89 ±0.03 AB 2055.99 ±41.25 11.06 ±0.44 AB
3100 161.23 ±2.73 B 0.93 ±0.03 AB 2079.06 ±47.24 12.01 ±0.48 A
Interaction: Strain × Metabolism energy levels (Kcal./Kg) :
Desert quail 2700 173.92 ±6.46 AB 0.97 ±0.05 AB 1951.78 ±72.54 10.90 ±0.59 AB
2800 172.48 ±1.17 AB 0.95 ±0.01 ABC 2012.15 ±13.75 11.10 ±0.16 AB
2900 160.04 ±8.84 B 0.79 ±0.06 C 1933.52 ±106.83 9.56 ±0.82 B
3000 163.29 ±2.93 AB 0.86 ±0.02 BC 2040.24 ±36.63 10.73 ±0.23 AB
3100 157.42 ±1.89 B 0.90 ±0.05 ABC 2030 ±24.41 11.58 ±0.68 AB
White quail 2700 183.39 ±5.07 A 1.02 ± 0.02 AB 2058.02 ±56.97 11.43 ±0.32 AB
2800 183.04 ±5.02 A 1.04 ±0.05 A 2135.31 ±58.56 12.18 ±0.65 A
2900 161.55 ±4.85 B 0.90 ±0.03 ABC 1951.77 ±58.60 10.83 ±0.42 AB
3000 165.81 ±7.19 AB 0.91 ±0.06 ABC 2071.74 ±89.91 11.40 ±0.82 AB
3100 165.03 ±5.58 AB 0.97 ±0.05 AB 2128.12 ±46.26 12.45 ±0.72 A
a,c: Mean in the same column with no common superscripts differ significantly (P<0.05)
Table 4.Effect of treatments and interaction on Protein intake, Protein conversion ratio, ME intake, and ME conversion ratio in the research (1-42 days)

Table 5 shows the significant effects (P≤0.05) of the three factors on carcass weight. The strain factor had the strongest effect on the desert quail in the T3 ME level, and the interaction between strain and ME levels was in the T3 group. The T5 ME level and the interaction factors had a significant effect (P≤0.05) on the edible giblet percentage. The results did not record any significant effects on dressing percentage caused by the three factors (Tables 6 and 7).

Treatments Carcass weight (g/bird) Dressing (%) Breast (%) Edible giblets (%)
Strain effect
Desert quail 130.65 ±2.63 A 71.10 ±0.63 31.82 ±1.39 5.77 ±0.20
White quail 118.57 ±2.03 B 68.89 ±0.80 31.51 ±1.17 5.50 ±0.15
Metabolism energy levels effect (Kcal./Kg) :
2700 121.92 ±3.44 B 69.17 ±1.45 31.59 ±1.35 5.16 ±0.35 B
2800 122.42 ±3.62 B 69.65 ±0.78 31.63 ±2.47 5.61±0.16 AB
2900 134.48 ±5.24 A 71.46 ±1.27 32.04 ±1.06 5.57 ±0.13 AB
3000 125.65 ±3.88 AB 69.93 ±1.48 31.94 ±0.97 5.74 ±0.35 AB
3100 118.59 ±3.43 B 69.77 ±0.95 31.14 ±0.77 6.12 ±0.30 A
Interaction : Strain × Metabolism energy levels(Kcal./Kg) :
Desert quail 2700 127.54 ±5.83 B 70.24 ±1.20 31.95 ±2.23 5.25 ± 0.57 AB
2800 128.27 ±10.40 B 70.85 ±1.02 31.88 ±1.51 5.52 ±0.25 AB
2900 144.87 ±9.29 A 72.85 ±1.55 32.19 ±1.06 5.70 ±0.25 AB
3000 131.89 ±7.64 AB 71.61 ±0.25 32.03 ±0.97 5.88 ±0.44 AB
3100 120.68 ±8.36 B 69.93 ±1.73 31.07 ±1.25 6.49 ±0.25 A
White quail 2700 116.30 ±10.43 B 68.10 ±2.87 31.22 ±0.38 5.06 ±0.46 B
2800 116.57 ±10.99 B 68.44 ±3.64 31.37 ±1.47 5.69 ±0.25 AB
2900 124.08 ±3.83 B 70.07 ±0.53 31.90 ±0.67 5.43 ±0.22 AB
3000 119.39 ±8.13 B 68.25 ±1.70 31.85 ±1.11 5.59 ±0.54 AB
3100 116.50 ±4.17 B 69.60 ±1.76 31.20 ±0.85 5.75 ±0.30 AB
a,c: Mean in the same column with no common superscripts differ significantly (P<0.05)
Table 5.Effect of treatments and interaction on carcass weight, dressing percentage, and edible giblets percentage in the research (42 days)
Treatments Tenderness Juiciness Flavor Acceptability
Strain effect
Desert quail 2.76 ±0.05 2.66 ±0.06 2.73 ±0.07 2.71 ±0.09
White quail 2.83 ±0.08 2.76 ±0.08 2.80 ±0.08 2.80 ±0.06
Metabolism energy levels effect (Kcal./Kg) :
2700 2.91 ±0.08 2.81 ±0.10 2.88 ±0.10 2.94 ±0.11
2800 2.94 ±0.09 2.91 ±0.06 2.82 ±0.06 2.88 ±0.09
2900 2.85 ±0.07 2.72 ±0.14 2.78 ±0.06 2.75 ±0.11
3000 2.72 ±0.11 2.60 ±0.06 2.72 ±0.07 2.63 ±0.07
3100 2.66 ±0.06 2.54 ±0.04 2.63 ±0.06 2.60 ±0.08
Interaction: Strain × Metabolism energy levels (Kcal./Kg) :
Desert quail 2700 2.88 ±0.09 2.81 ±0.07 2.81 ±0.09 2.94 ±0.10
2800 2.94 ±0.10 2.81 ±0.12 2.69 ±0.12 2.81 ±0.09
2900 2.81 ±0.08 2.69 ±0.09 2.75 ±0.07 2.69 ±0.07
3000 2.69 ±0.09 2.56 ±0.07 2.75 ±0.07 2.56 ±0.06
3100 2.50 ±0.07 2.44 ±0.05 2.63 ±0.06 2.56 ±0.05
White quail 2700 2.94 ±0.12 2.81 ±0.13 2.94 ±0.10 2.94 ±0.10
2800 2.94 ±0.12 3.00 ±0.11 2.94 ±0.10 2.94 ±0.10
2900 2.88 ±0.13 2.75 ±0.10 2.81 ±0.09 2.81 ±0.07
3000 2.75 ±0.11 2.63 ±0.09 2.69 ±0.08 2.69 ±0.09
3100 2.63 ±0.10 2.63 ±0.09 2.63 ±0.05 2.63 ±0.07
Table 6.Effect of treatments and interaction on some sensory meat tastes in the research (42 days)
Treatments Total Protein (gm/100ml) Albumin (gm/100ml) Globulin (gm/100ml) Triglycerides (mg/100ml) Total cholesterol (mg/100ml)
Strain effect
Desert quail 4.38 ±0.08 2.52 ±0.05 A 1.86 ±0.02 625.20 ±7.05 A 182.23±3.49
White quail 4.26 ±0.09 2.30 ±0.04 B 1.96 ±0.02 608.28 ±5.95 B 175.01±2.67
Metabolism energy levels effect(Kcal./Kg) :
2700 4.26 ±0.04 2.33 ±0.03 BC 1.93 ±0.03 587.50 ±5.15 C 171.32±3.83 B
2800 4.24 ±0.08 2.27 ±0.06 C 1.97 ±0.03 595.34± 5.18 C 172.66 ±4.22 AB
2900 4.29 ±0.15 2.37 ±0.07 BC 1.92 ±0.05 614.17± 5.90 B 177.45± 4.15 AB
3000 4.35 ±0.06 2.46 ±0.07 B 1.89 ±0.04 627.91± 5.46 B 184.07± 5.77 AB
3100 4.46 ±0.23 2.62 ±0.07 A 1.84±0.03 658.77± 7.03 A 187.60±5.33 A
Interaction : Strain × Metabolism energy levels(Kcal./Kg) :
Desert quail 2700 4.32 ±0.07 2.38 ±0.04 BCD 1.94 ±0.05 594.19 ±6.94 A 173.22 ±6.78
2800 4.17 ±0.14 2.43 ±0.04 BCD 1.74 ±0.05 598.92 ±9.17 DE 175.62 ±4.84
2900 4.45 ±0.06 2.49 ±0.08 BC 1.96 ±0.06 625.42 ±5.19 BC 181.15 ±7.34
3000 4.41 ±0.10 2.58 ±0.08 AB 1.83 ±0.06 635.65 ±6.51 BC 189.81 ±7.94
3100 4.55 ±0.09 2.73 ±0.11 A 1.82 ±0.06 671.80 ±7.66 A 191.37 ±4.54
White quail 2700 4.20 ±0.05 2.28 ±0.04 DE 1.92 ±0.05 580.81 ±6.79 E 169.42 ±4.47
2800 4.30 ±0.06 2.11 ±0.04 E 2.19 ±0.04 591.76 ±5.69 E 169.70 ±7.36
2900 4.12 ±0.15 2.25 ±0.06 DE 1.87 ±0.08 602.92 ±9.31 DE 173.75 ±4.25
3000 4.29 ±0.05 2.34 ±0.07 CD 1.95 ±0.07 620.17 ±7.56 CD 178.33 ±8.46
3100 4.37 ±0.36 2.50 ±0.06 BC 1.87 ±0.03 645.74 ± 7.68 B 183.83 ±3.46
a,c: Mean in the same column with no common superscripts differ significantly (P<0.05)
Table 7.Effect of treatments and interaction on some biochemical traits of blood in the research (1-42 days)

Table 8 shows the economic efficiency of producing 1 Kg live weight of grower quail in this study. The results showed that the desert quail has the best net revenue, compared to the white quail, and that T3 (2900 Kcal) had the best net revenue, compared to other ME levels, as the results of interaction for T3 (desert quail and 2900 Kcal) has best net revenue.

Treatments Feed intake cost Other cost Total cost Revenue Net Revenue %Net revenue: Total costs
Strain effect :
Desert quail 2437 2628 5065 9000 3935 77.69
White quail 2472 2652 5124 9000 3876 75.64
Metabolism energy levels effect (Kcal./Kg) :
2700 2649 2766 5415 9000 3585 66.20
2800 2522 2666 5188 9000 3812 73.48
2900 2235 2501 4736 9000 4264 90.03
3000 2396 2588 4984 9000 4016 80.58
3100 2472 2682 5154 9000 3846 74.62
Interaction: Strain × Metabolism energy levels (Kcal./Kg) :
Desert quail 2700 2833 2891 5724 9000 3276 57.23
2800 2410 2623 5033 9000 3967 78.82
2900 2081 2361 4442 9000 4558 102.61
3000 2225 2557 4782 9000 4218 88.21
3100 2638 2709 5347 9000 3653 68.32
White quail 2700 2465 2641 5106 9000 3894 76.26
2800 2634 2709 5343 9000 3657 68.44
2900 2389 2640 5029 9000 3971 78.96
3000 2567 2618 5185 9000 3815 73.58
3100 2306 2654 4960 9000 4040 81.45
Iraqi Dinar/Kg live weight
Table 8.Effect of treatments and interaction on economic efficiency in the research (1-42 days)

The desert quail strain recorded the best net revenue and better performance traits, compared to the white quail. In addition, the T3 ME level recorded the best net revenue and better performance traits than other ME levels, and the interaction T3 has the best net revenue and best performance traits in this study.

Authors' Contribution

Study concept and design: Y. G. K.

Acquisition of data: R. N. W.

Analysis and interpretation of data: M. M. K.

Drafting of the manuscript: R. N. W.

Critical revision of the manuscript for important intellectual content: R. N. W.

Statistical analysis: Y. G. K.

Administrative, technical, and material support: M. M. K.

Ethics

All the ethical procedures were approved by the ethics committee of the University of Mosul, Mosul, Iraq.

Conflict of Interest

The authors declare that they have no conflict of interest.

Acknowledgment

The authors are very grateful to the University of Mosul/College of Agriculture and Forestry for their provided facilities, which helped to improve the quality of this study.

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