Effect of Culture Medium on in vitro Fertilization in Local Iraqi Ewes

Document Type : Original Articles

Authors

1 Animal Production, College of Agriculture, University of Anbar, Al-Anbar, Iraq

2 College of Veterinary Medicine, University of Fallujah, Al-Anbar, Iraq

Abstract

in vitro fertilization (IVF) is considered to be the most important reproductive biotechnological method having great potential to accelerate genetic improvement in ruminants as well as for research on embryonic development. The present study aimed to investigate the effect of culture medium and the addition of natural and synthetic antioxidants on in vitro maturation (IVM), fertilization (IVF), and culture (IVC) in local Iraqi ewes. A total of 304 reproductive systems of local ewes were collected from a slaughterhouse in Fallujah, Anbar Province, Iraq from 3, January to 1, July 2021. The study was conducted in the Reproductive Biotechnology Laboratory, Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Fallujah, Iraq. A total of 1368 oocytes were recovered from 608 ovaries surrounded by cumulus cells. The method of collection was aspiration and oocytes were divided into eight treatments. The first (T1), the second (T2), the third (T3), the fourth (T4), the fifth (T5), the sixth (T6), the seventh (T7), and the eighth (T8) treatments were MEM + Capparis spinosa extract 50µmol, MEM + Silymarin extract 100µmol, MEM + Coenzyme Q10 5 µmol, MEM only serves as a control, DMEM + Capparis spinosa extract 50 µmol, DMEM + Silymarin extract 100µmol, DMEM + Coenzyme Q10 5µmol and DMEM only serves as a control, respectively. The results indicated a significant difference (P≤0.05) between T5 (DMEM + Capparis spinosa extract) and other controls or treatments. Cultural medium DMEM with Capparis spinosa extract (as an antioxidant) presents the best results in the morula and blastocyst stage.

Keywords

References

  1. Gordon I. Laboratory production of cattle embryos: Cabi; 2003.
  1. Majeed A, Al-Timimi I, Al Saigh M. In vitro embryo production from oocyte recovered from live and dead Iraqi black goat: A preliminary study. Res J Biotechnol. 2019;14:226-33.
  2. Dadashpour Davachi N, Norouzi E, Didarkhah M, Eslampanah M, Hablolvarid MH. In vitro Production of Grivet Monkey (C hlorocebus aethiops) Embryo. Iran J Vet Med. 2022;16(3):257-64.
  3. Dadashpour Davachi N. Effect of different surgical methods in the mouse embryo transfer: Electrosurgery versus cold surgical technique effects on repeated use of surrogate mothers, pregnancy rate and post-surgical behavior. Vet Res Forum. 2021;12(4):467-71.
  4. Ventura-Juncá P, Irarrázaval I, Rolle AJ, Gutiérrez JI, Moreno RD, Santos MJ. In vitro fertilization (IVF) in mammals: epigenetic and developmental alterations. Scientific and bioethical implications for IVF in humans. Biol Res. 2015;48(1):1-13.
  5. Dadashpour Davachi N, Kohram H, Zainoaldini S. Cumulus cell layers as a critical factor in meiotic competence and cumulus expansion of ovine oocytes. Small Rumin Res. 2012;102(1):37-42.
  6. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005;3(1):1-21.
  7. Gardner D, Kelley R. Impact of the IVF laboratory environment on human preimplantation embryo phenotype. J Dev Orig Health Dis. 2017;8(4):418-35.
  8. Zuelke KA, Jeffay SC, Zucker RM, Perreault SD. Glutathione (GSH) concentrations vary with the cell cycle in maturing hamster oocytes, zygotes, and pre‐implantation stage embryos. Mol Reprod Dev. 2003;64(1):106-12.
  9. Agarwal A, Allamaneni SS. Role of free radicals in female reproductive diseases and assisted reproduction. Reprod Biomed Online. 2004;9(3):338-47.
  10. Truong T, Gardner D. Antioxidants improve IVF outcome and subsequent embryo development in the mouse. Hum Reprod. 2017;32(12):2404-13.
  11. Rios J, Recio M, Villar A. Antimicrobial activity of selected plants employed in the Spanish Mediterranean area. Journal of ethnopharmacology. 1987;21(2):139-52.
  12. Shiau RJ, Shih PC, Wen YD. Effect of silymarin on curcumin-induced mortality in zebrafish (Danio rerio) embryos and larvae. Indian J Exp Biol. 2011;49(7):491-7.
  13. Talevi R, Barbato V, Fiorentino I, Braun S, Longobardi S, Gualtieri R. Protective effects of in vitro treatment with zinc, d-aspartate and coenzyme q10 on human sperm motility, lipid peroxidation and DNA fragmentation. Reprod Biol Endocrinol. 2013;11(1):1-10.
  14. Wani N, Wani G, Khan M, Salahudin S. Effect of oocyte harvesting techniques on in vitro maturation and in vitro fertilization in sheep. Small Rumin Res. 2000;36(1):63-7.
  15. Dadashpour Davachi N, Fallahi R, Dirandeh E, Liu X, Bartlewski PM. Effects of co-incubation with conspecific ampulla oviductal epithelial cells and media composition on cryotolerance and developmental competence of in vitro matured sheep oocytes. Theriogenology. 2018;120:10-5.
  16. Duncan DB. Multiple Range and Multiple F Tests. Biometrics. 1955;11(1):1-42.
  17. Caleja C, Barros L, Antonio AL, Oliveira MBP, Ferreira IC. A comparative study between natural and synthetic antioxidants: Evaluation of their performance after incorporation into biscuits. Food Chem. 2017;216:342-6.
  18. Kharche S, Goel P, Kumar Jha B, Goel A, Jindal S. Factors influencing in-vitro embryo production efficiency of caprine oocytes: A review. Indian J Anim Sci. 2011;81(4):344.
  19. Torres-Osorio V, Urrego R, Echeverri-Zuluaga JJ, López-Herrera A. Oxidative stress and antioxidant use during in vitro mammal embryo production, Review. Rev Mex Cienc Pecu. 2019;10(2):433-59.
  20. de Oliveira Santos MV, Borges AA, de Queiroz Neta LB, Bertini LM, Pereira AF. Use of natural antioxidants in in vitro mammalian embryo production. Semin Cienc Agrar. 2018;39(1):431-43.
  21. Zullo G, Albero G, Neglia G, De Canditiis C, Bifulco G, Campanile G, et al. L-ergothioneine supplementation during culture improves quality of bovine in vitro–produced embryos. Theriogenology. 2016;85(4):688-97.
  22. Baghiani A, Ameni D, Boumerfeg S, Adjadj M, Djarmouni M, Charef N, et al. Studies of antioxidants and xanthine oxidase inhibitory potentials of root and aerial parts of medicinal plant Capparis spinosa L. Am J Med Sci. 2012;2(1):25-32.
  23. Kalantari H, Foruozandeh H, Khodayar MJ, Siahpoosh A, Saki N, Kheradmand P. Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide-induced acute liver damage in mice. J Tradit Complement Med. 2018;8(1):120-7.
  24. Ochiai T, Shimeno H, Mishima K-i, Iwasaki K, Fujiwara M, Tanaka H, et al. Protective effects of carotenoids from saffron on neuronal injury in vitro and in vivo. Biochim Biophys Acta Gen Subj. 2007;1770(4):578-84.
  25. Khoshniat MT, Towhidi A, Rezayazdi K, Zhandi M, Rostami F, Dadashpour Davachi N, et al. Dietary omega-3 fatty acids from linseed oil improve quality of post-thaw but not fresh sperm in Holstein bulls. Cryobiology. 2020;93:102-8.
  26. de Souza Rocha-Frigoni NA, da Silva Leão BC, Nogueira É, Accorsi MF, Mingoti GZ. Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture. Zygote. 2015;23(2):159-68.
  27. Natarajan R, Bhawani S, Munuswamy D. Effect of L-ascorbic acid supplementation at different gaseous environments on in vitro development of preimplantation sheep embryos to the blastocyst stage. Anim Reprod. 2018;7(1):21-8.
  28. Gonçalves FdS, Barretto L, Arruda RPd, Perri SHV, Mingoti GZ. Effect of antioxidants during bovine in vitro fertilization procedures on spermatozoa and embryo development. Reprod Domest Anim. 2010;45(1):129-35.
  29. Maside C, Martinez CA, Cambra JM, Lucas X, Martinez EA, Gil MA, et al. Supplementation with exogenous coenzyme Q10 to media for in vitro maturation and embryo culture fails to promote the developmental competence of porcine embryos. Reprod Domest Anim. 2019;54:72-7.
Archives of Razi Institute
Volume 77, Issue 5 - Serial Number 5
September and October 2022
Pages 1561-1567

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