Evaluating the Level of Serum IL-23 in Brucellosis Infection by ELISA and Investigating its Relationship in Cases with Failure to Respond to Treatment

Document Type : Original Articles

Authors

1 Department of Pharmacy, Al-Hadba University College, Mosul, Iraq

2 College of technical engineering, The Islamic University, Najaf, Iraq

3 Department of Optics Techniques, Dijlah University College, Al-Masafi Street, Al-Dora, Baghdad 00964, Iraq

4 Medical laboratory techniques Department, Medical (Technology) College, Al-Farahidi University, Baghdad, Iraq

5 Al-Nisour University College, Baghdad, Iraq

6 Anesthesia Techniques Department, Al-Mustaqbal University College, Babylon, Iraq

7 Al-Manara College For Medical Sciences, Misan, Iraq

8 Al-Esraa University College, Baghdad, Iraq

9 College of Health and Medical Technology, Al-Ayen University, Thi-Qar, Iraq

10 Department of Pharmacy, Al-Zahrawi University College, Karbala, Iraq

11 Medical Laboratory Techniques Department, Hilla University College, Babylon, Iraq

Abstract

Brucella is belonging to the small immobile gram-negative spore-lacking cocco-bacilli bacteria family that grows in an aerobic environment, it is known as a zoonosis infection named brucellosis. This study was designed to investigate serum values of IL-23 in patient with brucellosis and investigate its relationship with cases with failure to respond to conventional medical therapy. A total of 372 individuals were divided into 2 groups (n=186) as follows: Group A comprising 186 infected participants with brucella (7-80 years-old), these people had not received antibiotics for at least 6 months ago. Group B including the healthy participants. All the participants in both groups were in the same age range. 5 ml blood samples were obtained from the participants intravenously (without anticoagulation substance). The serum level of IL-23 was investigated by ELISA diagnostic kit. The recorded data showed that the levels of IL-23 in the serum samples obtained from group A (143.64 Pg/ml) significantly (P<0.001) increased compared with this value in group B (23.14 Pg/ml). Based on the recorded data in the forms completed by all the participants at the day 0 of the experiment, 44 out of 186 individuals in group A, had experienced Brucellosis attack 2-3 times in spite of receiving medical prescriptions. A hypothesis about the possible immune system disorders in these participants lead us to did the re-sampling following drug administration. Results illustrated failure to respond to conventional medical therapy in patients with low level of serum IL-23.

Keywords

Main Subjects

References

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Archives of Razi Institute
Volume 77, Issue 3 - Serial Number 3
May and June 2022
Pages 1275-1279

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