The Effectiveness of Biotin (Vitamin B7) Added to the Diet in Improving the Efficiency of Productivity, and Some Physiological Traits for Broiler Chickens (Ross-308) Exposed to Oxidative Stress

Document Type : Original Articles

Authors

1 Department of Animal Production, College of Agriculture, Al-Qasim Green University, Baghdad, Iraq

2 College of Water Resources Engineering, Al-Qasim Green University, Baghdad, Iraq

3 Department of Physiology, College of Veterinary Medicine, Al-Qasim Green University, Baghdad, Iraq

Abstract

Biotin (B7) acts as an antioxidant, as it inhibits the effect of many free radicals that are naturally formed within the organism's body. This study aimed to determine the effect of adding different concentrations of biotin to the diet of broilers exposed to oxidative stress in improving productive and physiological performance. 180 unsexed Ross-308 one-day age chicks of broiler chickens were used, and they were reared together until 7 days. Then the chicks were randomly distributed into 5 treatments; each treatment included 3 replicates, 12 chicks for each replicate. Oxidative stress was induced by adding 0.5% H2O2 to drinking water. The first treatment, T1 (control), was free from adding B7 or H2O2, and the second treatment (T2): was a positive control treatment free from adding vitamin B7 + water added to it H2O2 at an average of 0.5%. The third treatment (T3): adding 550 micrograms of B7/kg of feed + water added H2O2 at an average of 0.5%. Fourth treatment (T4): 650 micrograms/kg vitamin B7 + water with 0.5% H2O2 added on average. Fifth treatment (T5): 750 micrograms of vitamin B7/kg of feed + 0.5% H2O2 added to the water. The results of adding vitamin B7 to the diet of T4 and T5 birds exposed to oxidative stress showed a significant increase (P<0.05) in the average live body weight, total weight gain and cumulative feed consumption average, and the best cumulative feed conversion ratio for treated birds (T3, T4, T5). In comparison to the other therapies, all additional vitamin B7 treatments demonstrated a significant decrease (P<0.05) in the concentrations of glucose, cholesterol, triglycerides, as well as Malondialdehyde (MDA), and low-density lipoproteins (LDL) and a significant increase (P<0.05) in the concentrations of high-density lipoproteins (HDL) and total protein. The concentration of glutathione peroxidase (GSH-Px) was significantly elevated (P<0.05) in the serum of T5-treated birds compared to all other treatments. We conclude that adding vitamin B7 in different concentrations improved the productivity and some physiological traits of broilers exposed to the induced oxidative stress compared with the negative and positive control treatments.

Keywords

Main Subjects

References

  1. Grodner M, Escott-Stump S, Dorner S. Nutritional Foundations and Clinical Applications: A Nursing Approach: Elsevier - Health Sciences Division; 2019.
  2. Rodwell VW, Bender DA, Botham KM, Kennelly PJ, Weil PA. Harper's Illustrated Biochemistry 31e: McGraw-Hill Education; 2018.
  3. Cheng F-C, Jen J-F, Tsai T-H. Hydroxyl radical in living systems and its separation methods. J Chromatogr B. 2002;781(1-2):481-96.
  4. McDonald P. Animal Nutrition: Prentice Hall/Pearson; 2011.
  5. Khan R. Antioxidants and poultry semen quality. Worlds Poult Sci J. 2011;67(2):297-308.
  6. Combs G. Clinical implications of selenium and vitamin E in poultry nutrition. Vet Clin Nutr. 1994;1(3):133-40.
  7. McMahon RJ. Biotin in metabolism and molecular biology. Annual review of nutrition. 2002;22(1):221-39.
  8. Sastry GA. Vet Clin Pathol. 1983.
  9. Franey RJ, Amador E. Serum cholesterol measurement based on ethanol extraction and ferric chloride-sulfuric acid. Clin Chim Acta. 1968;21(2):255-63.
  1. Grundy SM, Cleeman JI, Bairey Merz CN, Brewer HB, Clark LT, Hunninghake DB, et al. Implications of recent clinical trials for the national cholesterol education program adult treatment panel III guidelines. J Am Coll Cardiol. 2004;44(3):720-32.
  2. Carlson LA. Determination of serum triglycerides. J Atheroscler Thromb. 1963;3:334-6.
  3. Wotton I. Micro–analysis in medical biochemistry 4 th ed. churchill Livingstone. London; 1974.
  4. Al-Qudah KM, Ismail ZB. The relationship between serum biotin and oxidant/antioxidant activities in bovine lameness. Res Vet Sci. 2012;92(1):138-41.
  5. Lai M, Biochemistry BUDo. Studies on Microsomal Lipid Peroxidation: Brunel University; 1982.
  6. Duncan DB. Multiple range and multiple F tests. Biometrics. 1955;11(1):1-42.
  7. Manthey KC, Griffin JB, Zempleni J. Biotin supply affects expression of biotin transporters, biotinylation of carboxylases and metabolism of interleukin-2 in Jurkat cells. J Nutr. 2002;132(5):887-92.
  8. Tufarelli V. Poultry Nutrition: MDPI AG; 2021.
  9. White III HB, Whitehead CC, Armstrong J. Relationship of biotin deposition in turkey eggs to dietary biotin and biotin-binding proteins. Poult Sci. 1987;66(7):1236-41.
  10. Oloyo RA. Studies on the biotin requirement of broilers fed sunflower seed meal based diets. Arch Anim Nutr. 1994;45(4):345-53.
  1. Quarantelli A, Cacchioli A, Romanelli S, Righi F, Alpigiani I, Gabbi C. Effects of different levels of dietary biotin on the performance and bone structure of broilers. Ital J Anim Sci. 2007;6(1):5-7.
  2. Spitzer V, Schweigert F. Vitamin basics the facts about vitamins in nutrition. Germany: DSM Nutritional Products Ltd. 2007.
  3. Chapman-Smith A, Cronan Jr JE. Molecular biology of biotin attachment to proteins. J Nutr. 1999;129(2):477-84.
  4. Montagut G, Bladé C, Blay M, Fernández-Larrea J, Pujadas G, Salvadó MJ, et al. Effects of a grapeseed procyanidin extract (GSPE) on insulin resistance. J Nutr Biochem. 2010;21(10):961-7.
  5. Sweetman L. Pantothenic acid and biotin. Biochemical and Physiological Aspects of Human Nutrition. 2000.
  6. Nelson DL, Cox M. Lehninger Principles of Biochemistry: International Edition: Macmillan Learning; 2017.
  7. Shehata AM, Yousef OM. Physiological studies on the risk factors responsible for atherosclerosis in rats. Nat Sci. 2010;8(5):144-51.
  8. Carnaghan R, Hebert CN, Patterson D, Sweasey D. Comparative biological and biochemical studies in hybrid chicks: 2. Susceptibility to aflatoxin and effects on serum protein constituents. Br Poult Sci. 1967;8(4):279-84.
  9. Oguntibeju O. Antioxidant-Antidiabetic Agents and Human Health: IntechOpen; 2014.
Archives of Razi Institute
Volume 77, Issue 5 - Serial Number 5
September and October 2022
Pages 1805-1812

Files

Share

How to cite

Statistics