Molecular Detection of Some Virulence Genes in Salmonella Species Isolated from Clinical Samples in Iraq

Document Type : Original Articles

Author

Department of Biology, College of Sciences, Mustansiriyah University, Baghdad, Iraq

Abstract

Salmonella species (spp.) are a major source of diarrheal diseases everywhere and one of the most dangerous foodborne bacteria. The present study aimed to detect the occurrence of the most important virulence genes in Salmonella enterica (S. enterica) among bacteria isolated from stool in Baghdad hospitals, Iraq. In total, 50 swab stool samples were collected from patients suffering from food poisoning, attending to different hospitals in Baghdad. The isolates were identified using morphological tests and were confirmed by the Vitek-2 system (BioMe´rieux, France). A genomic DNA kit (Qiagen, Germany) was utilized to extract DNA from the isolates. Molecular detection of five virulence genes, including invA, papC, spvC, stn, and fimH, was performed using Polymerase Chain Reaction (PCR). Out of 50 swab samples, 40% (20 samples) were confirmed as S. enterica. Moreover, the prevalence of virulence genes determined by the PCR demonstrated that all 20 S. enterica isolates carried at least one gene from those associated with biofilm formation. The invA, stn, and fimH were the most predominant genes existing in all 20 S. enterica isolates. The prevalence of papC and spvC virulence genes was 75% (15 out of 20) and 65% (13 out of 20), respectively. The current data support the occurrence of Salmonella spp. exhibiting a broad range of virulence genes in stool samples from patients who had food poisoning, which indeed makes these bacteria a significant threat to public health.

Keywords

Main Subjects

References

  1. Alarawi FA, Saeed EM. Isolation, Antibiogram and Molecular Detection of Mannheimia and Pasteurella Associated with Pneumonia in Sheep in Al-Madinah Region, Saudi Arabia. Int J Vet Sci. 2021;10(2):135-40.
  2. Abouelkhair A, Hussein A. Molecular Characterization of Different Salmonella Enterica Serotypes Isolated From Frozen Meat in Minoufiya Governorate. J Curr Vet Res. 2019;1(2):63-8.
  3. Galanis E, Lo Fo Wong DM, Patrick ME, Binsztein N, Cieslik A, Chalermchikit T, et al. Web-based surveillance and global Salmonella distribution, 2000-2002. Emerg Infect Dis. 2006;12(3):381-8.
  4. Hald T, Aspinall W, Devleesschauwer B, Cooke R, Corrigan T, Havelaar AH, et al. World Health Organization Estimates of the Relative Contributions of Food to the Burden of Disease Due to Selected Foodborne Hazards: A Structured Expert Elicitation. PLoS One. 2016;11(1):e0145839.
  5. Greger M. The human/animal interface: emergence and resurgence of zoonotic infectious diseases. Crit Rev Microbiol. 2007;33(4):243-99.
  6. Van TTH, Yidana Z, Smooker PM, Coloe PJ. Antibiotic use in food animals worldwide, with a focus on Africa: Pluses and minuses. J Glob Antimicrob Resist. 2020;20:170-7.
  1. Sotohy S, Khalifa E. Molecular Characterization of Some Virulence Genes of Salmonella enterica Serotype Sandow and Saintpaul Isolated from Environment of Dairy Farms at Assiut Province, Egypt. J Vet Sci Technol. 2018;09.
  2. Awad A, Gwida M, Khalifa E, Sadat A. Phenotypes, antibacterial-resistant profile, and virulence-associated genes of Salmonella serovars isolated from retail chicken meat in Egypt. Vet World. 2020;13(3):440-5.
  3. Kaabi H, Al-Yassari A. 16SrRNA sequencing as tool for identification of Salmonella spp isolated from human diarrhea cases. J Phys Conf Ser. 2019;1294:062041.
  4. Zhang Y, Hu X, Wang Q, Zhang Y. Recent advances in microchip-based methods for the detection of pathogenic bacteria. Chin Chem Lett. 2022;33(6):2817-31.
  5. Oliveira SD, Rodenbusch CR, Ce MC, Rocha SL, Canal CW. Evaluation of selective and non-selective enrichment PCR procedures for Salmonella detection. Lett Appl Microbiol. 2003;36(4):217-21.
  6. Birosova E, Siegfried L, Kmet'ova M, Makara A, Ostro A, Gresova A, et al. Detection of virulence factors in alpha-haemolytic Escherichia coli strains isolated from various clinical materials. Clin Microbiol Infect. 2004;10(6):569-73.
  7. Khaltabadi Farahani R, Ehsani P, Ebrahimi-Rad M, Khaledi A. Molecular Detection, Virulence Genes, Biofilm Formation, and Antibiotic Resistance of Salmonella enterica Serotype enteritidis Isolated from Poultry and Clinical Samples. Jundishapur J Microbiol. 2018;11(10): 69504.
  8. Tiba MR, Yano T, Leite Dda S. Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis. Rev Inst Med Trop Sao Paulo. 2008;50(5):255-60.
  9. Erdem B, Ercis S, Hascelik G, Gur D, Aysev AD. Antimicrobial resistance of Salmonella enterica group C strains isolated from humans in Turkey, 2000-2002. Int J Antimicrob Agents. 2005;26(1):33-7.
  10. Ben Aissa R, Al-Gallas N, Troudi H, Belhadj N, Belhadj A. Trends in Salmonella enterica serotypes isolated from human, food, animal, and environment in Tunisia, 1994-2004. J Infect. 2007;55(4):324-39.
  11. Tolba K. Microflora in locally processed frozen meat. Vet Med J Giza. 1994.
  12. Law JW, Ab Mutalib NS, Chan KG, Lee LH. Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. Front Microbiol. 2014;5:770.
  13. Zhao X, Lin CW, Wang J, Oh DH. Advances in rapid detection methods for foodborne pathogens. J Microbiol Biotechnol. 2014;24(3):297-312.
  14. Ahmed HA, El-Hofy FI, Shafik SM, Abdelrahman MA, Elsaid GA. Characterization of Virulence-Associated Genes, Antimicrobial Resistance Genes, and Class 1 Integrons in Salmonella enterica serovar Typhimurium Isolates from Chicken Meat and Humans in Egypt. Foodborne Pathog Dis. 2016;13(6):281-8.
  15. Uchiya KI, Kamimura Y, Jusakon A, Nikai T. Salmonella Fimbrial Protein FimH Is Involved in Expression of Proinflammatory Cytokines in a Toll-Like Receptor 4-Dependent Manner. Infect Immun. 2019;87(3).
  16. Kuzminska-Bajor M, Grzymajlo K, Ugorski M. Type 1 fimbriae are important factors limiting the dissemination and colonization of mice by Salmonella Enteritidis and contribute to the induction of intestinal inflammation during Salmonella invasion. Front Microbiol. 2015;6:276.
  17. Mahmoud MAM, Megahed G, Yousef MS, Ali FAZ, Zaki RS, Abdelhafeez HH. Salmonella Typhimurium Triggered Unilateral Epididymo-Orchitis and Splenomegaly in a Holstein Bull in Assiut, Egypt: A Case Report. Pathogens. 2020;9(4).
  18. Robertson J, Yoshida C, Kruczkiewicz P, Nadon C, Nichani A, Taboada EN, et al. Comprehensive assessment of the quality of Salmonella whole genome sequence data available in public sequence databases using the Salmonella in silico Typing Resource (SISTR). Microb Genom .2018;4(2).
  19. Bosch A, Gkogka E, Le Guyader FS, Loisy-Hamon F, Lee A, van Lieshout L, et al. Foodborne viruses: Detection, risk assessment, and control options in food processing. Int J Food Microbiol. 2018;285:110-28.
  20. Mezal EH, Sabol A, Khan MA, Ali N, Stefanova R, Khan AA. Isolation and molecular characterization of Salmonella enterica serovar Enteritidis from poultry house and clinical samples during 2010. Food Microbiol. 2014;38:67-74.
  21. Jajere SM. A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multidrug resistance. Vet World. 2019;12(4):504-21.
  22. Rito E, Recker T, Betts R, Lee A, Loisy-Hamon F, Gkogka E, et al., editors. Foodborne Viruses: Detection, Risk Assessment, and Control Options in Food Processing. IAFP European Symposium on Food Safety; 2019: IAFP.
  23. Bonardi S, Alpigiani I, Bruini I, Barilli E, Brindani F, Morganti M, et al. Detection of Salmonella enterica in pigs at slaughter and comparison with human isolates in Italy. Int J Food Microbiol. 2016;218:44-50.
Archives of Razi Institute
Volume 77, Issue 5 - Serial Number 5
September and October 2022
Pages 1741-1747

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