Molecular Detection of blaSHV-la Gene in Klebsiella pneumonia Isolated from Urinary Tract Infections, Najaf, Iraq

Document Type : Original Articles

Authors

1 Department of Medical Laboratory Techniques, Faculty of Medical Technologies, Islamic University, Najaf, Iraq

2 Directorate of Education, Ministry of Education, Najaf, Iraq

Abstract

Klebsiella pneumoniae is a gram-negative bacterium that causes serious illnesses, including pneumonia, liver abscess, meningitis, bloodstream infections, and urinary tract infections (UTIs). This study aimed to isolate and diagnose K. pneumoniae from clinical specimens of urine from patients with UTIs and perform molecular detection of the blaSHV-la gene in K. pneumonia in the Najaf Province, Iraq. The study included 100 clinical specimens from October 2021 to March 2022. As an initial diagnosis, K. pneumoniae isolates were diagnosed based on culture and biochemical features. Apart from the usage of polymerase chain reaction (PCR) technology to identify the blaSHV-la gene, the final diagnostic was achieved by the automated Vitek-2 compact system. The biochemical findings revealed that 40 out of every 100 isolates tested positive for K. pneumoniae. These results were validated by Vitek, which revealed that 40/100 of the samples tested positive for K. pneumoniae, and by PCR utilizing the blaSHV-la gene, which showed that 13/40 of the samples tested positive for K. pneumoniae isolated from the urine of patients with UTIs. In conclusion, the results indicated that the use of the Vitek-2 technique was required to confirm the accurate identification of the pathogen. Klebsiella pneumoniae clinical isolates showed multidrug resistance to antibiotics commonly used to treat UTIs. The blaSHV gene encoded for Extended-spectrum beta-lactam antibiotic was found almost in K. pneumoniae isolates.

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Main Subjects

References

  1. Lin W, Kao C, Yang D, Tseng C, Wu A, Teng C, et al. Clinical and microbiological characteristics of Klebsiella pneumoniae from community-acquired recurrent urinary tract infections. Eur J Clin Microbiol Infect Dis. 2014;33(9):1533-9.
  2. Kil K-S, Darouiche RO, Hull RA, Mansouri MD, Musher DM. Identification of a Klebsiella pneumoniae strain associated with nosocomial urinary tract infection. J Clin Microbiol. 1997;35(9):2370-4.
  1. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018;8:4.
  2. Tanner WD, VanDerslice JA, Goel RK, Leecaster MK, Fisher MA, Olstadt J, et al. Multi-state study of Enterobacteriaceae harboring extended-spectrum beta-lactamase and carbapenemase genes in US drinking water. Sci Rep. 2019;9(1):1-8.
  3. Köck R, Siemer P, Esser J, Kampmeier S, Berends MS, Glasner C, et al. Defining Multidrug Resistance of Gram-Negative Bacteria in the Dutch–German Border Region—Impact of National Guidelines. Microorganisms. 2018;6(1):11.
  4. Blazquez J, Morosini M-I, Negri M-C, Baquero F. Selection of naturally occurring extended-spectrum TEM β-lactamase variants by fluctuating β-lactam pressure. Antimicrob Agents Chemother. 2000;44(8):2182-4.
  5. Al-Kraety IAA, Alquraishi ZHO, Alsadawi AA. Molecular Study of Fimh Gene in Klebisella Pneumoniae Isolated From Urinary Catheter Patients. Indian J Forensic Med Toxicol. 2020;14(2).
  6. Ghasemi Y, Archin T, Kargar M, Mohkam M. A simple multiplex PCR for assessing prevalence of extended-spectrum β-lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz, Iran. Asian Pac J Trop Med. 2013;6(9):703-8.
  7. Krishnamurthy V, Vijaykumar G, Kumar S, Prashanth H, Prakash R, Nagaraj E. Phenotypic and genotypic methods for detection of extended spectrum β lactamase producing Escherichia coli and Klebsiella pneumoniae isolated from ventilator associated pneumonia. J Clin Diagn Res. 2013;7(9):1975.
  8. Amin A, Ghumro P, Hussain S, Hameed A. Prevalence of antibiotic resistance among clinical isolates of Klebsiella pneumoniae isolated from a Tertiary Care Hospital in Pakistan. Malays J Microbiol. 2009;5(2):81-6.

11.          Kumar V, Sun P, Vamathevan J, Li Y, Ingraham K, Palmer L, et al. Comparative genomics of Klebsiella pneumoniae strains with different antibiotic resistance profiles. Antimicrob Agents Chemother. 2011;55(9):4267-76.

Archives of Razi Institute
Volume 77, Issue 3 - Serial Number 3
May and June 2022
Pages 1181-1184

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