Characterizing the BHK-21 C5 cell line and determining cellular sensitivity to rubella virus compared with the routine cell (RK13)

Document Type : Original Articles

Authors

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Bio bank, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975-148, Karaj, Iran

Abstract

The World Health Organization has strict rules and recommendations on the selection and use of cell substrates in laboratories. Given the widespread use of safe and secure cell substrates in the production and quality control of viral vaccines and also the high demand for vaccines against viral diseases, obligating the selection of a suitable cell substrate for cultivation and production of biological products. Animal cell lines play a valuable role in the preparation and propagation of viral seeds; thus, the current study used the BHK-21 cell line among others for viral checking with the aim of replacing the BHK-21 C5 cell line with the RK13 cell line to investigate the cytopathic effects of the rubella virus. To this end, attempts were made to determine the characteristics of the BHK-21 C5 cell line including cell growth characteristics and sterility tests to validate its safety and security. Then, by culturing the cells in a 96-well microplate, titration of the rubella virus was subsequently performed by preparing serial dilutions of the virus from 10-1 to 10-5 and inoculated to cell lines in order to compare the sensitivity of BHK-21 C5 and RK13 cell lines to rubella virus. Data analysis according to the results of the tests by ahead default, p-value < 0/05 was equal to p-value = 0.01 based on SPSS analysis with the paired-sample t-test. In addition, the box-plot diagram indicated a significant difference between these cell lines. Based on the results, the BHK-21 C5 cell line seems to be more sensitive to the rubella virus than others. Therefore, it can be used for production and quality control of the vaccine and in research and diagnosis of rubella.

Keywords

Main Subjects

Article Title [French]

Caractériser la Lignée Cellulaire BHK-21 C5 et Déterminer la Sensibilité Cellulaire au Virus de la Rubéole par Rapport à la Cellule de Routine (RK13)

Abstract [French]

L'Organisation mondiale de la santé a des règles et des recommandations strictes sur la sélection et l'utilisation de substrats cellulaires dans les laboratoires. L'utilisation généralisée de substrats cellulaires sûrs et sécurisés dans la production et le contrôle de la qualité des vaccins viraux ainsi que la forte demande de vaccins contre les maladies virales nous obligent à sélectionner un substrat cellulaire approprié pour la culture et la production de produits biologiques. Les lignées cellulaires animales jouent un rôle précieux dans la préparation et la propagation des graines virales; ainsi, la présente étude a utilisé la lignée cellulaire BHK-21 entre autres pour le contrôle viral dans le but de remplacer la lignée cellulaire BHK-21 C5 par la cellule RK13 pour étudier les effets cytopathiques du virus de la rubéole. À cette fin, des tentatives ont été faites pour déterminer les caractéristiques de la lignée cellulaire BHK-21 C5, y compris les caractéristiques de croissance cellulaire et les tests de stérilité pour valider sa sûreté et sa sécurité. Ensuite, en cultivant les cellules dans une microplaque à 96 puits, le titrage du virus de la rubéole a ensuite été effectué en préparant des dilutions en série du virus de 10-1 à 10-5 et inoculé à des lignées cellulaires afin de comparer la sensibilité de BHK-21 lignées cellulaires C5 et RK13 au virus de la rubéole. L'analyse des données selon les résultats des tests par défaut anticipé, la valeur p<0/05 était égale à la valeur p = 0.01 sur la base de l'analyse SPSS avec le test t pour échantillons appariés. De plus, le diagramme en boîte a indiqué une différence significative entre ces lignées cellulaires. D'après les résultats, la lignée cellulaire BHK-21 C5 semble être plus sensible au virus de la rubéole que les autres. Par conséquent, elle peut être utilisée pour la production et le contrôle qualité du vaccin et dans la recherche et le diagnostic de la rubéole.

Keywords [French]

  • caractérisation
  • lignée cellulaire BHK-21 C5
  • virus de la rubéole
  • test de titrage
  • lignée cellulaire RK13

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Archives of Razi Institute
Volume 76, Issue 3
September 2021
Pages 461-469

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