First report of Gallibacterium isolation from layer chickens in Iran

Document Type : Original Articles

Authors

1 Department of avian Bacterial Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran

2 Department of Veterinary Disease Biology, Faculty of Health Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark

3 Kowsar Agricultural Research & Development Company, Tehran, Iran

Abstract

Gallibacterium, a gram-negative coccobacillus, is a genus of the Pasteurellaceae family with capability of infecting a wide range of avian host species. In 2003, Gallibacterium was selected instead of different previous names. Four different species has been recognized in Gallibacterium genus so far. Members of the genus can cause a wide range of pathological lesions, from upper respiratory tract lesions, follicle degeneration, enteritis,  pericarditis, hepatitis, oophoritis, septicemia to more important  problems such as salpingitis and peritonitis. Salpingitis and peritonitis of layer farms caused by Gallibacterium result in decreased egg-production and increased mortality. Gallibacterium is the most common single bacterial infection in egg-laying farms with reproductive disorders. During a microbiological survey on bacterial infectious agents of reproductive tract of layers, some isolates from salpingitis cases were suspicious of Gallibacterium. According to culture and biochemical properties, the primary identification was in support of genus Gallibacterium. A polymerase chain reaction (PCR) test using primers specific for the genus identification was carried out. The suspected isolate showed bands of 970 and 1080 bp, which are specific for Gallibcterium. To confirm the identification, a positive sample was sent to University of Copenhagen for repeating the PCR test. To our knowledge, this is the first report of isolation and identification of Gallibacterium in Iran. This study shows the possibility of a role for Gallibacterium in poultry industry of Iran. This study also shows the need for further investigations on epidemiological situation of the infection, as well as the isolation and identification of different species of the genus Gallibacterium.  

Keywords

Main Subjects

Article Title [French]

Premier rapport sur l'isolation de Gallibacterium de la couche des poulets en Iran

Abstract [French]

Gallibacterium, un cocobacillus gram-négatif est un genre de la famille des Pasteurellaceae capable d'infecter un large éventail d'espèces hôtes aviaires. En 2003, Gallibacterium a été sélectionné au lieu de noms antérieurs différents. Quatre espèces différentes ont été reconnues dans le genre Gallibacterium jusqu'à présent. Les membres du genre peuvent causer un large éventail de lésions pathologiques, des lésions des voies respiratoires supérieures, la dégénérescence des follicules, l'entérite, la péricardite, l'hépatite, l'oophrite, la septicémie à des problèmes plus importants tels que la salpingite et la péritonite. La salpingite et la péritonite des fermes de couche causées par Gallibacterium entraînent une diminution de la production d'œufs et une mortalité accrue. La gallibactérie est l'infection bactérienne unique la plus fréquente dans les fermes pondeuses atteintes de troubles de la reproduction. Au cours d'une étude microbiologique sur les agents infectieux bactériens des couches reproductives, certains isolats provenant de cas de salpingite étaient soupçonnés de Gallibacterium. Selon la culture et les propriétés biochimiques, l'identification primaire était à l'appui du genre Gallibacterium. Un test de réaction en chaîne par polymérase (PCR) utilisant des amorces spécifiques à l'identification du genre a été effectué. L'isolat soupçonné a montré des bandes de 970 et 1080 pb, qui sont spécifiques pour Gallibcterium. Pour confirmer l'identification, un échantillon positif a été envoyé à l'Université de Copenhague pour répéter le test de PCR. À notre connaissance, c'est le premier rapport d'isolation et d'identification de Gallibacterium en Iran. Cette étude montre la possibilité d'un rôle pour Gallibacterium dans l'industrie avicole d'Iran. Cette étude montre également la nécessité de nouvelles recherches sur la situation épidémiologique de l'infection, ainsi que sur l'isolation et l'identification de différentes espèces du genre Gallibacterium.

Keywords [French]

  • Gallibacterium
  • Salpinigite
  • Poulet
  • Isolation
  • Réaction en chaîne par polymérase (PCR)

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Archives of Razi Institute
Volume 72, Issue 2
July and August 2017
Pages 125-130

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