A study on the genetic analysis of clinical isolates and vaccine strains of Bordetella pertussis by pulsed-field gel electrophoresis (PFGE)

Document Type : Original Articles

Authors

1 Department of Microbiology, Faculty of Veterinnary, Islamic Azad University of Karaj, Karaj, Iran

2 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran

Abstract

Considering the circulation of Bordetella pertussis clinical strains among populations with high vaccination coverage, it is necessary to have a proper understanding of this bacterium causing whooping cough. Various techniques, which are available for studying B. pertussis, can facilitate a proper comparison between different populations. We genotypically analyzed a collection of two vaccine strains used for the production of killed pertussis vaccine during 2000-2014 at Razi Vaccine and Serum Research Institute, Karaj, Iran. Ten clinical and two reference (Tohama 1 and 18323) strains were used by means of pulsed-field gel electrophoresis (PFGE). The genetic profiles of the vaccine master and working seeds showed no significant changes in the frequency of fingerprint types in the vaccine strains; also, the homogeneity of the profiles was demonstrated. However, the clinical isolates showed heterogeneity in the genetic profiles. In addition, serotyping was performed with monoclonal antisera to agglutinogens 2 and 3. Analysis of the fimbriae showed that all ten clinical strains expressed Fim3.

Keywords

Article Title [French]

L’analyse génétique des isolats cliniques et souches vaccinales de Bordetellapertussis par électrophorèse en champ pulsé (ECP)

Abstract [French]

Etant donnée la circulation des souches de Bordetellapertussisau sein des populations avec une couverture vaccinale élevée, une bonne compréhension de la nature de cette bactérie provoquant la coqueluche est nécessaire. Une variété de techniques ont été mises en place afin de faciliter la caractérisation des souches de Bordetellapertussisau sein des différentes populations. Une analyse génotypique a été menée sur une collection de deux souches vaccinales utilisées entre les années 2000 et 2014dans la production de vaccins inactivés de la coqueluche à l’Institut de Recherche sur les Vaccins et les Sérums Razi (Karaj, Iran). Au total, 10 isolats cliniques et 2 souches de références (Tohama 1 et 18323) ont été analysés par électrophorèse en champ pulsé (ECP). Selon nos résultats, le profil génétique de la souche-mère et des semences actives ne montre aucun changement significatif dans la fréquence des types d’empreintes observée avec les souches vaccinales. Ceci reflète donc la bonne homogénéité des profils étudiés. De plus, un sérotypage a été effectué avec des antisérums monoclonaux des agglutinogènes 2 et 3. L’analyse des fimbriae a révélé que les 10 isolats cliniques exprimaient Fim3.

Keywords [French]

  • Bordetella pertussis
  • Empreintes ADN
  • ECP
  • XbaI

References

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Archives of Razi Institute
Volume 71, Issue 4
November and December 2016
Pages 219-225

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