Neospora caninum (N. caninum) is an apicomplexan parasite and causes abortion and congenital neosporosis in cattle worldwide. In this study, we evaluate the virulence of a N. caninum isolate on mouse strain C57BL/6. Six groups of five mice C57BL/6 were intraperitoneally inoculated with 1 × l07, 1.5 × l07, 2 × l07, 3 × l07 and 4 × l07 tachyzoites and a control inoculum of DMEM, respectively. Clinical signs and mortality rate were recorded and confirmed by histopathological findings and molecular method. The results of this study indicated that LD50 was 2.5 × l07 tachyzoites of N. caninum per C57BL/6 mouse. This can be used as a lethal challenge model in vaccine development studies.
Namavari, M., Sayari, M., Kargar, M., Lotfi, M., & Kamalzadeh, M. (2012). Virulence assessment of a Neospora caninum isolate for inbred C57BL/6 mouse. Archives of Razi Institute, 67(1), 57-61. doi: 10.22092/ari.2016.103888
MLA
M.M. Namavari; M. Sayari; M. Kargar; M. Lotfi; M. Kamalzadeh. "Virulence assessment of a Neospora caninum isolate for inbred C57BL/6 mouse". Archives of Razi Institute, 67, 1, 2012, 57-61. doi: 10.22092/ari.2016.103888
HARVARD
Namavari, M., Sayari, M., Kargar, M., Lotfi, M., Kamalzadeh, M. (2012). 'Virulence assessment of a Neospora caninum isolate for inbred C57BL/6 mouse', Archives of Razi Institute, 67(1), pp. 57-61. doi: 10.22092/ari.2016.103888
VANCOUVER
Namavari, M., Sayari, M., Kargar, M., Lotfi, M., Kamalzadeh, M. Virulence assessment of a Neospora caninum isolate for inbred C57BL/6 mouse. Archives of Razi Institute, 2012; 67(1): 57-61. doi: 10.22092/ari.2016.103888
)
