Cloning of fusion (F) protein gene of peste des petits ruminants virus (PPRV) in secretory Pichia pastoris vector

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Abstract

  With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cloned into pPICZαA a secretory expression vector of P. pastoris for first time. The insertion was proved by both production of a 218 bp segment in Nested PCR and isolation of gene from construct by restriction enzyme (XbaΙ). Finally, It was sequenced. In conclusion, after the expression of fusion (F) gene in p. pastoris expression system, it can be used in production of recombinant vaccine against PPR disease.  

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Archives of Razi Institute
Volume 62, Issue 4
November and December 2007
Pages 215-221

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