Expression Analysis of PVT-1 lncRNA and TGF-β in Colorectal Cancer and Non-Cancerous Colorectal Polyps: A Case-Control Study

Document Type : Original Articles

Authors

Department of Biology, NT.C., Islamic Azad University, Tehran, Iran

10.22092/ari.2025.369700.3686

Abstract

Colorectal cancer ranks among the most prevalent types of cancer across the globe, particularly in developing countries. Biomarkers like long non-coding RNAs (lncRNAs) have a key impact in early detection and personalized treatment. This study aimed to compare the expression levels of PVT-1 lncRNA and TGF-β gene in colorectal cancer tissues and non-cancerous polyp tissues. Fifty colorectal cancer tissue samples and fifty non-cancerous polyp tissues, confirmed by a gastroenterologist, were analyzed. RNA was extracted, cDNA was synthesized, and expression levels of PVT-1 lncRNA and TGF-β were quantified using real-time PCR. Data analysis was performed with SPSS software, and all experiments were conducted under identical laboratory conditions to ensure reliability. The colorectal cancer. A total of 50 participants (22 men and 28 women) were enrolled, with a mean age of 62.56 ± 16.41 years, while the control group consisted of 30 males and 20 females (mean age: 58.41 ± 11.13 years). Expression analysis revealed significantly higher levels of both PVT-1 lncRNA (fold change = X.X, P < 0.05) and TGF-β (fold change = X.X, P < 0.05) in colorectal cancer tissues compared to controls. These differences persisted despite demographic imbalances between groups. ROC curve analysis demonstrated the diagnostic potential of both biomarkers in distinguishing cancerous from non-cancerous tissues. Our findings suggest that PVT-1 lncRNA and TGF-β may serve as promising molecular biomarkers for colorectal cancer, with potential diagnostic utility. Further validation studies are warranted to establish their role in clinical practice.

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Archives of Razi Institute
Volume 80, Issue 6
In-Press
November and December 2025

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