Concurrent Occurrence of Infectious Bursal Disease and Multicausal Respiratory Infections Caused by Newcastle Disease and Avian Metapneumovirus in Broilers

Document Type : Original Articles

Authors

Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia, Iran

Abstract

Control strategy of respiratory complex infections should address precipitating and predisposing causative agents in general and immunosuppressive agents in particular. In both clinical and subclinical forms, infectious bursal disease virus (IBDV) is one of the most immunosuppressive diseases of young chickens. This study aimed to investigate the concurrent occurrence of subclinical infectious bursal disease (IBD) and multicausal respiratory complex infections caused by Newcastle disease virus (NDV) and avian metapneumovirus (aMPV) in broilers. In this study, 800 tissue samples (e.g., trachea, cecal tonsil, bursa of Fabricius, and spleen) and 400 sera samples were collected from broilers with confirmed respiratory signs selected from 20 broiler farms in west Azerbaijan province, Iran, from October 2018 to February 2019. Pathogens in the tissue samples were detected using RT-PCR for the VP2 gene of IBDV, F gen of NDV, and N gene of aMPV. The amplified products were sequenced afterward. At the end of the husbandry period, sera samples were used to detect antibodies against IBDV, aMPV, and NDV using ELISA and HI tests. Molecular results showed that the 45% (9/20), 30% (6/20), and 15% (3/20) of tissue samples were positive for IBDV, NDV, and aMPV, respectively. Regarding co-infection, 5% (1/20) of farm isolates were positive for IBD and ND, while 10% (2/20) of farms isolates were positive for IBD and aMPV. Co-infection of IBD, ND, and aMPV was not detected in farm isolates. Serological results indicated that the IBD co-infected flocks had almost higher (P<0.05) antibody titers against IBD; however, IBDV-NDV co-infected flocks and IBDV-aMPV co-infected flocks had lower antibody titer against NDVand aMPV, respectively. It can be concluded that lower antibody titer against ND and aMPV in IBD-ND and IBD-aMPV co-infections indicated suppressive effects of IBD on these diseases. Therefore, vaccination against IBD even in regions without clinical form of IBD is inevitable for the reduction of immunosuppressive effects of subclinical IBD on immune responses against these diseases.

Keywords

References

  1. Abdelaziz AM, Mohamed MHA, Fayez MM, Al-Marri T, Qasim I, Al-Amer AA. Molecular survey and interaction of common respiratory pathogens in chicken flocks (field perspective). Vet World. 2019;12(12):1975-86.
  2. Collett S, Smith J. Principle of disease prevention, diagnosis and control. Diseases of poultry. 14th ed: John Wiley & Son; 2020. p. 4-40.
  3. Dey S, Pathak DC, Ramamurthy N, Maity HK, Chellappa MM. Infectious bursal disease virus in chickens: prevalence, impact, and management strategies. Vet Med . 2019;10:85-97.
  4. Eterradossi N, Saif YM. Infectious Bursal Disease. Diseases of Poultry.2020. p. 257-83.
  5. Umar S, Munir MT, Ahsan U, Raza I, Chowdhury MR, Ahmed Z, et al. Immunosuppressive interactions of viral diseases in poultry. Worlds Poult Sci J. 2017;73(1):121-35.
  6. Rautenschlein S. Avian metapneumovirus. Diseases of poultry. 14th ed: John Wiley & Son, Inc; 2020. p. 135-66.
  7. Allahyari E, Allymehr M, Molouki A, MEHRABADI MF, Talebi A. Molecular Characterisation And Phylogenetic Study Of The Fusion Gene Of Newcastle Disease Viruses Isolated From Broiler Farms Of Iran In 2018-2019. Bulg J Vet Med. 2022;25(1).
  8. Haji-Abdolvahab H, Ghalyanchilangeroudi A, Bahonar A, Ghafouri SA, Vasfi Marandi M, Mehrabadi MHF, et al. Prevalence of avian influenza, Newcastle disease, and infectious bronchitis viruses in broiler flocks infected with multifactorial respiratory diseases in Iran, 2015-2016. Trop Anim Health Prod. 2019;51(3):689-95.
  9. Jabbarifakhar M, Mousavi F, Ziafati Z, Rezaee H, Hosseini H, Fallah M. Emergence of genotype VIIg of velogenic Newcastle disease virus in Iran 2018: The First Report. Iranian J Virol. 2018;12:40-6.
  10. Miller P, Koch G. Newcastle disease. In: DE S, editor. Diseases of poultry. 14th ed: John Wiley & Son; 2020. p. 112-29.
  11. Ranjbar VR, Mohammadi A, Dadras H. Infectious bursal disease virus suppresses H9N2 avian influenza viral shedding in broiler chickens. Br Poult Sci. 2019;60(5):493-8.
  12. Spackman E, Stephens CB, Pantin-Jackwood MJ. The Effect of Infectious Bursal Disease Virus-Induced Immunosuppression on Vaccination Against Highly Pathogenic Avian Influenza Virus. Avian Dis. 2018;62(1):36-44.
  13. Saif Y, Jackwood D, . Infectious bursal disease. In: Williams S, editor. A laboratory manual for the isolation, identification, and characterization of avian pathogens. 6th ed: American Association of Avian Pathologists; 2016. p. 239-42.
  14. Tulu D. Epidemiology status and economic importance of infectious bursal disease in poultry production, Ethiopia. Epidemiol Int J. 2019;3(3):1-13.
  15. Samy A, Naguib MM. Avian Respiratory Coinfection and Impact on Avian Influenza Pathogenicity in Domestic Poultry: Field and Experimental Findings. Vet Sci. 2018;5(1).
  16. Creelan JL, Graham DA, McCullough SJ. Detection and differentiation of pathogenicity of avian paramyxovirus serotype 1 from field cases using one-step reverse transcriptase-polymerase chain reaction. Avian Pathol. 2002;31(5):493-9.
  17. Lin Z, Kato A, Otaki Y, Nakamura T, Sasmaz E, Ueda S. Sequence comparisons of a highly virulent infectious bursal disease virus prevalent in Japan. Avian Dis. 1993;37(2):315-23.
  18. Mase M, Yamaguchi S, Tsukamoto K, Imada T, Imai K, Nakamura K. Presence of avian pneumovirus subtypes A and B in Japan. Avian Dis. 2003;47(2):481-4.
  19. OIE. Newcastle disease: OIE Terrestrial Manual; 2021.
  20. Umar S, Teillaud A, Aslam HB, Guerin JL, Ducatez MF. Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016. BMC Vet Res. 2019;15(1):351.
  21. Taifebagherlu J, Talebi A, Allymehr M. Concurrent occurrence of infectious bursal disease and respiratory complex caused by infectious bronchitis and avian influenza (H9N2) in broilers. BJVM; 2021.
  22. Li L, Kubasova T, Rychlik I, Hoerr FJ, Rautenschlein S. Infectious bursal disease virus infection leads to changes in the gut associated-lymphoid tissue and the microbiota composition. PLoS One. 2018;13(2):0192066.
  1. Norouzian H, Farjanikish G, Hosseini H. Genetic and pathologic characteristics of infectious bursal disease viruses isolated from broiler chickens in Iran during 2014-2015. Acta Virol. 2017;61(2):191-6.
  2. Cardoso W, Aguiar Filho J, Romão J, Salles R, Câmara S, Siqueira A, et al. Interference of infectious bursal disease virus on antibody production against Newcastle disease and infectious bronchitis virus. Braz J Poult Sci.2006;8(3):177-82.
  3. Fan L, Wang Y, Jiang N, Chen M, Gao L, Li K, et al. Novel variant infectious bursal disease virus suppresses Newcastle disease vaccination in broiler and layer chickens. Poult Sci. 2020;99(12):6542-8.
  4. Batista IA, Hoepers P, Silva MFB, Nunes PLF, Diniz DCA, Freitas A, et al. Circulation of Major Respiratory Pathogens in Backyard Poultry and their Association with Clinical Disease and Biosecurity. Braz J Poult Sci. 2020;22.
Archives of Razi Institute
Volume 77, Issue 3 - Serial Number 3
May and June 2022
Pages 1007-1016

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