Reduce the Changes in Histological Features of Colon Resulting from Induced Ulcerative Colitis in Mice by Worm Antigens

Document Type : Original Articles

Authors

1 Department of Biology, College of Sciences, Diyala University, Baqubah, Iraq

2 Department of Biology, College of Education for Pure Sciences, Diyala University, Baqubah, Iraq

Abstract

Ulcerative colitis is one of the inflammatory bowel disease (IBD) consequences characterized by chronic inflammation of the gastrointestinal (GI) tract. The current study aims to determine the changes in colon tissue caused by induced Ulcerative Colitis and reduce these changes by worms' antigen. The study included 45 adult male albino mice (Mus musculus), with ages ranging between 8-10 weeks; the average weights ranged between 18-32 g. Ulcerative colitis was induced by intracolonic administration of 100 μL of 4% after they had been starved for 18 h. The animals were dissected after one week, and routine histological sections were conducted. The results of the histological study showed that in the colon of white mice treated with 4% acetic acid occurred, many histological changes were represented by the appearance of inflammatory cells in the mucous layer around the goblet cells, and the formation of vacuoles, necrosis at epithelium and intense congestion under the surface epithelium. The marked histological sections of the colon in mice with induced ulcerative colitis treated with helminth extract at a concentration of 0.1 mg/kg for a week, were showed that the histological changes began to decrease in the colon tissue, with the presence of a few inflammatory cells as well as little mucus secretion.

Keywords

References

  1. Xavier RJ, Podolsky DK. Unravelling the pathogenesis of inflammatory bowel disease. Nature. 2007;448(7152):427-34.
  2. Bramhall M, Florez-Vargas O, Stevens R, Brass A, Cruickshank S. Quality of methods reporting in animal models of colitis. Inflamm Bowel Dis. 2015;21(6):1248-59.
  3. Alves de Almeida AC, de-Faria FM, Dunder RJ, Manzo LP, Souza-Brito AR, Luiz-Ferreira A. Recent Trends in Pharmacological Activity of Alkaloids in Animal Colitis: Potential Use for Inflammatory Bowel Disease. Evid Based Complement Alternat Med. 2017;2017:8528210.
  4. MacPherson BR, Pfeiffer CJ. Experimental production of diffuse colitis in rats. Digestion. 1978;17(2):135-50.
  5. Boirivant M, Fuss IJ, Chu A, Strober W. Oxazolone colitis: A murine model of T helper cell type 2 colitis treatable with antibodies to interleukin 4. J Exp Med. 1998;188(10):1929-39.
  6. Kawada M, Arihiro A, Mizoguchi E. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease. World J Gastroenterol. 2007;13(42): 5581-93.
  7. Lean QY, Eri RD, Fitton JH, Patel RP, Gueven N. Fucoidan Extracts Ameliorate Acute Colitis. PLoS One. 2015;10(6): 0128453.
  8. Valdez R, Appelman HD, Bronner MP, Greenson JK. Diffuse duodenitis associated with ulcerative colitis. Am J Surg Pathol. 2000;24(10):1407-13.
  9. Kurutas EB, Cetinkaya A, Bulbuloglu E, Kantarceken B. Effects of antioxidant therapy on leukocyte myeloperoxidase and Cu/Zn-superoxide dismutase and plasma malondialdehyde levels in experimental colitis. Mediators 2005;2005(6):390-4.
  10. Nell S, Suerbaum S, Josenhans C. The impact of the microbiota on the pathogenesis of IBD: lessons from mouse infection models. Nat Rev Microbiol. 2010;8(8):564-77.
  11. Wirtz S, Neurath MF. Mouse models of inflammatory bowel disease. Adv Drug Deliv Rev. 2007;59(11):1073-83.
  12. Keubler LM, Buettner M, Hager C, Bleich A. A Multihit Model: Colitis Lessons from the Interleukin-10-deficient Mouse. Inflamm Bowel Dis. 2015;21(8):1967-75.
  13. Hartmann RM, Morgan Martins MI, Tieppo J, Fillmann HS, Marroni NP. Effect of Boswellia serrata on antioxidant status in an experimental model of colitis rats induced by acetic acid. Dig Dis Sci. 2012;57(8):2038-44.
  14. Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL. Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology. 1989;96(3):795-803.
  15. Wang A, McKay DM. Immune modulation by a high molecular weight fraction from the rat tapeworm Hymenolepis diminuta. Parasitology. 2005;130(5): 575-85.
  16. Vanlangenakker N, Vanden Berghe T, Krysko DV, Festjens N, Vandenabeele P. Molecular mechanisms and pathophysiology of necrotic cell death. Curr Mol Med. 2008;8(3):207-20.
  1. Lichtenstein GR, Rutgeerts P. Importance of mucosal healing in ulcerative colitis. Inflamm Bowel Dis. 2010;16(2):338-46.
  2. Ardizzone S, Cassinotti A, Duca P, Mazzali C, Penati C, Manes G, et al. Mucosal healing predicts late outcomes after the first course of corticosteroids for newly diagnosed ulcerative colitis. Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association. 2011;9(6):483-9.
  3. Gunther C, Martini E, Wittkopf N, Amann K, Weigmann B, Neumann H, et al. Caspase-8 regulates TNF-alpha-induced epithelial necroptosis and terminal ileitis. Nature. 2011;477(7364):335-9.
  4. Fleming JO, Isaak A, Lee JE, Luzzio CC, Carrithers MD, Cook TD, et al. Probiotic helminth administration in relapsing-remitting multiple sclerosis: a phase 1 study. Mult Scler. 2011;17(6):743-54.
Archives of Razi Institute
Volume 77, Issue 5 - Serial Number 5
September and October 2022
Pages 1995-1999

Files

Share

How to cite

Statistics