Effect of Oregano Essential Oil Combined with Live and Killed Newcastle Disease Vaccines on Immune Response in Broilers Chicks in Erbil, Iraq: A Comparative Study

Document Type : Original Articles


1 Department of Veterinary Microbiology, College of Veterinary Medicine, Kufa University, Kufa, Iraq

2 Faculty of Agriculture, Salahaddin University, Erbil, Kurdistan, Iraq


This trial was conducted to evaluate the effects of oregano essential oil supplementation at the concentration of 50 mL/1000L of drinking water on immune response and growth performance in broiler chickens. A total of 390 one-day-old Rose chickens were housed together in six experimental boxes (n=65 each), including non-vaccinated groups and non-vaccinated oregano treated groups. Group A: vaccinated with LaSota vaccine via eye drop at 5-, 15-, and 28-day-old adding oregano essential oil in drinking water 1 day post-vaccination. Group B: vaccinated with LaSota vaccine via eye drop at 5-, 15-, 28-day-old only without oregano essential oil. Group C: vaccinated with inactivated Newcastle disease (ND) vaccine at 5-day-old via subcutaneous (SC) injection one dose adding oregano essential oil in drinking water 1 day post-vaccination for 3 days. Group D: vaccinated with inactivated ND vaccine at 5-day-old via SC injection one dose only without oregano essential oil. Group E: non-vaccinated as the control group. Group F: non-vaccinated and adding oregano essential oil for 3 days in drinking water as the control group. The effect of oregano essential oil in drinking water combined with two forms of live and killed ND virus vaccines on the immune responses and serum proteins was investigated in broiler chickens. The results showed the significant effect of oregano on the immune response of chicks in groups D and C that were vaccinated with the killed vaccine at 23- and 33-day-old. Based on the findings, a peak was recorded at 38-day-old with a significant difference (P<0.05) in vaccinated and non-vaccinated groups, as well as a significant increase in 38-day-old for group C (P<0.05), compared to other groups regarding the oregano effects on the immune response of groups. In addition, an increase in the serum protein was significantly different (P>0.05) in the vaccinated broiler chicks, and serum globulin showed a quantitative increase in the vaccinated broiler chicks, compared with non-vaccinated groups. However, the results showed that the effect of oregano was non-significantly different (P>0.05) and it had no beneficial effect on growth performance. The results of this study showed the effects of commercial Oregano supplementation (50 mL/1000L drinking water), which increased serum total protein, including globulin, and provoked the best immunological response to ND vaccinations in broiler chickens. More research is needed to develop a state that may beneficially affect the health and uniformity of chickens.


Main Subjects

  1. Al-Garib S, Gielkens A, Gruys E, Kochi G. Review of Newcastle disease virus with particular references to immunity and vaccination. Worlds Poult Sci J. 2003;59(2):185-200.
  2. Ahmed A. Comparison of the immune response between local manufactured and commercial inactivated Newcastle Disease Virus vaccine in a challenge trail with field isolated Newcastle Disease Virus. Iraqi J Vet Med. 2018;42(1):46-51.
  3. Conan A, Goutard FL, Sorn S, Vong S. Biosecurity measures for backyard poultry in developing countries: a systematic review. BMC Vet Res. 2012;8(1):1-10.
  4. Miller PJ, Koch G. Newcastle disease. Diseases of poultry. 2013;13:89-138.
  5. Otim MO, Mukiibi-Muka G, Christensen H, Bisgaard M. Aflatoxicosis, infectious bursal disease and immune response to Newcastle disease vaccination in rural chickens. Avian Pathol. 2005;34(4):319-23.
  6. Sabrin M, Saha S, Amin M. Immune Response Capability Ofshuvra Chicken. Bangladesh J Vet Med. 2012;10(1-2):1-7.
  7. Suarez DL, Miller PJ, Koch G, Mundt E, Rautenschlein S. Newcastle disease, other avian paramyxoviruses, and avian metapneumovirus infections. Dis Poultry. 2020:109-66.
  8. Ratcliffe MJ, Härtle S. B cells, the bursa of Fabricius, and the generation of antibody repertoires. Avian immunology: Elsevier; 2022. p. 71-99.
  9. Michalska-Sionkowska M, Walczak M, Sionkowska A. Antimicrobial activity of collagen material with thymol addition for potential application as wound dressing. Polym Test.. 2017;63:360-6.
  10. Sharifi‚ÄźRad M, Varoni EM, Iriti M, Martorell M, Setzer WN, del Mar Contreras M, et al. Carvacrol and human health: A comprehensive review. Phytother Res. 2018;32(9):1675-87.
  11. Naghibi F, Mosadegh M, Mohammadi MS, Ghorbani A. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. 2005.
  12. Ali H, Al-Khalifa AR, Aouf A, Boukhebti H, Farouk A. Effect of nanoencapsulation on volatile constituents, and antioxidant and anticancer activities of Algerian Origanum glandulosum Desf. essential oil. Sci Rep. 2020;10(1):1-9.
  13. Zakaria Nabti L, Sahli F, Laouar H, Olowo-Okere A, Nkuimi Wandjou JG, Maggi F. Chemical composition and antibacterial activity of essential oils from the Algerian endemic Origanum glandulosum Desf. against multidrug-resistant uropathogenic E. coli isolates. Antibiotics. 2020;9(1):29.
  14. Harfi B, Benahmed A, Karkour L. Characterization of Origanum glandulosum desf. essential oils collected from different culture conditions towards standardized ex situ production. J Essent Oil-Bear Plants. 2019;22(3):838-50.
  15. Mechergui K, Jaouadi W, Coelho J, Serra MC, Khouja M. Biological activities and oil properties of Origanum glandulosum Desf: a review. Phytothérapie. 2016;14(2):102-8.
  16. Hanson R, Hofstad M. Newcastle disease. In" Diseases of Poultry". Iowa State University Press, Ames, Iowa. 1978;50010:619-56.
  17. Aiello SE, Moses MA, Allen DG. The Merck veterinary manual: Merck & Company, Incorporated; 2016.
  18. Alagawany M, El-Hack M, Farag MR, Tiwari R, Dhama K. Biological effects and modes of action of carvacrol in animal and poultry pro-duction and health-a review. Adv Anim Vet Sci. 2015;3(2s):73-84.
  19. Cross D, McDevitt R, Hillman K, Acamovic T. The effect of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age. Br Poult Sci. 2007;48(4):496-506.
  20. Lee K-W, Everts H, Kappert H, Frehner M, Losa R, Beynen A. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. Br Poult Sci. 2003;44(3):450-7.
  21. Tizard IR. Veterinary Immunology-E-Book: Elsevier Health Sciences; 2017.
  22. Waheed U, Siddique M, Arshad M, Ali M, Saeed A. Preparation of Newcastle Disease Vaccine from VG/GA Strain and its Evaluation in Commercial Broiler Chicks. Pak J Zool. 2013;45(2).
  23. Rehman S, Arshad M, Waheed U, Rehman K. Preparation and evaluation of oilemulsified Newcastle disease vaccine from VG/GA strain of Newcastle Disease virus. Ind J Pl Sci. 2002;1:401-5.
  24. Folitse R, Halvorson D, Sivanandan V. Efficacy of combined killed-in-oil emulsion and live Newcastle disease vaccines in chickens. Avian Dis. 1998:173-8.
  25. Botsoglou N, Christaki E, Florou-Paneri P, Giannenas I, Papageorgiou G, Spais A. The effect of a mixture of herbal essential oils or α-tocopheryl acetate on performance parameters and oxidation of body lipid in broilers. S Afr J Anim Sc. 2004;34(1):52-61.
  26. Lillehoj HS, Kim DK, Bravo DM, Lee SH, editors. Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens. BMC Proceed; 2011: BioMed Central.
  27. Hashemipour H, Kermanshahi H, Golian A, Veldkamp T. Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poultry Sci. 2013;92(8):2059-69.
  1. Acamovic T, Brooker J. Biochemistry of plant secondary metabolites and their effects in animals. Proc Nutr Soc. 2005;64(3):403-12.
  2. Andrade LN, De Sousa DP. A review on anti-inflammatory activity of monoterpenes. Molecules. 2013;18(1):1227-54.
  3. Bassett R. Oregano’s positive impact on poultry production. World Poultry-Elsevier. 2000;16(9):31-4.
  4. Hernandez F, Madrid J, Garcia V, Orengo J, Megias M. Influence of two plant extracts on broilers performance, digestibility, and digestive organ size. Poultry Sci. 2004;83(2):169-74.
  5. Jassim SA, Naji MA. Novel antiviral agents: a medicinal plant perspective. J Appl Microbiol. 2003;95(3):412-27.
  6. dos Santos Schmidt EM, Paulillo AC, Locatelli-Dittrich R, Beltrame O, Denadai J. Serum protein profiles of juvenile ring-necked pheasants vaccinated or not against Newcastle disease. Int J Poult Sci. 2009;8(4):359-62.
  7. Zachary JF, McGavin MD. Pathologic Basis of Veterinary Disease5: Pathologic Basis of Veterinary Disease: Elsevier Health Sciences; 2012.
  8. Oladele S, Nok A, Esievo K, Abdu P, Useh N. Haemagglutination inhibition antibodies, rectal temperature and total protein of chickens infected with a local Nigerian isolate of velogenic Newcastle disease virus. Vet Res Commun. 2005;29(2):171-9.
  9. Nnadi P, Eze P, Ezema W. Influence of delayed feeding on the performance, development and response of immune system to Newcastle disease vaccination in chickens. Int J Poult Sci. 2010.