Subfertility significantly affects couples and their families and challenges obstetrics and gynecologists with long-term treatment. Leptin has been widely studied in different types of infertility. However, the results of previous studies are contradictory and the role of leptin is still debated in the reproductive process. The present study aimed to investigate the relationship between serum leptin concentration and some reproductive hormones and endometrial thickness in women with primary subfertility. The present case-control study was conducted from December 2020 to July 2021. A total of 100 women with primary subfertility and 100 fertile women participated in this study as a control group. Blood samples were collected and ultrasonography examinations were performed on all patients. The recorded data indicated that the mean leptin level was higher in women with subfertility compared to that of the control group, 26.8±15.2 ng/mL and 6.4±2.3 ng/mL, respectively (P<0.001). Leptin level significantly increased with a higher body mass index (P<0.001). Leptin at a level of 11 ng/ml or higher is a significant predictor of infertility (odds ratio=2.793). Leptin levels showed high sensitivity, specificity, and accuracy of 96%, 98%, and 96.9%, respectively as predictors of subfertility. As a result, a high leptin level was a strong and valid predictor of subfertility.
Hussain R, Nasir F, Hashim N, Naqvi S. Subfertility: aetiological factors. Professional Med J. 2017;24(07):1012-5.
Lainez NM, Coss D. Obesity, neuroinflammation, and reproductive function. Endocrinology. 2019;160(11):2719-36.
Ramos-Lobo AM, Donato Jr J. The role of leptin in health and disease. Temperature. 2017;4(3):258-91.
Kalaiselvi V, Saikumar P, Prabhu K. The anti mullerian hormone-a novel marker for assessing the ovarian reserve in women with regular menstrual cycles. J Clin Diagnostic Res. 2012;6(10):1636.
Pérez-Pérez A, Sánchez-Jiménez F, Maymó J, Dueñas JL, Varone C, Sánchez-Margalet V. Role of leptin in female reproduction. Clin Chem LabMed. 2015;53(1):15-28.
Anwar AA, Abdullah N, Padjalangi AN, Hamid F, Mappeware NA, Lukas E. Serum leptin concentration is correlated to insulin resistance in polycystic ovary syndrome (PCOS) patients. Mol Cell Biomed Sci. 2021;5(2):93-7.
Kargasheh FB, Ansaripour S, Borumandnia N, Moradi N, Zandieh Z, Maleki M, et al. Association of leptin G2548A and leptin receptor Q223R polymorphisms and their serum levels with infertility and recurrent pregnancy loss in Iranian women with polycystic ovary syndrome. PLoS One. 2021;16(8):0255920.
Park H-K, Ahima RS. Physiology of leptin: energy homeostasis, neuroendocrine function and metabolism. Metabolism. 2015;64(1):24-34.
Silvestris E, de Pergola G, Rosania R, Loverro G. Obesity as disruptor of the female fertility. Reprod Biol Endocrinol. 2018;16(1):1-13.
Salem AM. Variation of Leptin During Menstrual Cycle and Its Relation to the Hypothalamic–Pituitary–Gonadal (HPG) Axis: A Systematic Review. Int J Womens Health. 2021;13:445.
Kawwass JF, Summer R, Kallen CB. Direct effects of leptin and adiponectin on peripheral reproductive tissues: a critical review. Mol Hum Reprod. 2015;21(8):617-32.
Sylvia KE, Lorenz TK, Heiman JR, Demas GE. Physiological predictors of leptin vary during menses and ovulation in healthy women. Reprod Biol. 2018;18(1):132-6.
Childs GV, Odle AK, MacNicol MC, MacNicol AM. The importance of leptin to reproduction. Endocrinology. 2021;162(2):204.
Baig M, Azhar A, Rehman R, Syed H, Tariq S, Gazzaz ZJ. Relationship of serum leptin and reproductive hormones in unexplained infertile and fertile females. Cureus. 2019;11(12).
Chow VT, Phoon M. Measurement of serum leptin concentrations in university undergraduates by competitive ELISA reveals correlations with body mass index and sex. Adv Physiol Educ. 2003;27(2):70-7.
Al-Jawadi ZA. Effect of Leptin on the Infertile Women. Ann Rom Soc Cell Biol. 2020:111-6.
Gambineri A, Laudisio D, Marocco C, Radellini S, Colao A, Savastano S. Female infertility: which role for obesity? Int J Obes Suppl. 2019;9(1):65-72.
Maharlouei N, Morshed Behbahani B, Doryanizadeh L, Kazemi M. Prevalence and pattern of infertility in Iran: A systematic review and meta-analysis study. Women’s Health Bull. 2021:63-71.
Ananth CV, Schisterman EF. Confounding, causality, and confusion: the role of intermediate variables in interpreting observational studies in obstetrics. Am J Obstet Gynecol. 2017;217(2):167-75.
Kumari P, Jaiswar S, Shankhwar P, Deo S, Ahmad K, Iqbal B, et al. Leptin as a predictive marker in unexplained infertility in north indian population. J Clin Diagnostic Res. 2017;11(3):QC28.
Tafvizi F, Masomi M. Comparison of serum leptin level in women with unexplained infertility and fertile women in Iran. J Obstet Gynaecol India. 2016;66(1):466-70.
Alao M. Association between Serum Leptin and AntiMullerian Hormones in Women with Infertility in Ogbomoso Southwest Nigeria. Niger J Clin Pract. 2020.
Chou SH, Mantzoros C. Role of leptin in human reproductive disorders. J Endocrinol. 2014;223(1):49-62.
Kamyabi Z, Gholamalizade T. A comparative study of serum and follicular fluid leptin concentrations among explained infertile, unexplained infertile and fertile women.Int J Fertil Steril. 2015;9(2):150.
Wertel I, Gogacz M, Polak G, Jakowicki J, Kotarski J. Leptin is not involved in the pathophysiology of endometriosis-related infertility. Eur J Obstet Gynecol. 2005;119(2):206-9.
Nikanfar S, Oghbaei H, Rezaei YR, Zarezadeh R, Jafari-Gharabaghlou D, Nejabati HR, et al. Role of adipokines in the ovarian function: oogenesis and steroidogenesis. J Steroid Biochem Mol Biol. 2021;209:105852.
Isidori AM, Strollo F, Morè M, Caprio M, Aversa A, Moretti C, et al. Leptin and aging: correlation with endocrine changes in male and female healthy adult populations of different body weights. J Clin Endocrinol Metab. 2000;85(5):1954-62.
Zhong N, Wu X-P, Xu Z-R, Wang A-H, Luo X-H, Cao X-Z, et al. Relationship of serum leptin with age, body weight, body mass index, and bone mineral density in healthy mainland Chinese women. Clin Chim Acta. 2005;351(1-2):161-8.
Senghor A, Shivashekar M, Vinodhini V. Serum Leptin And Adiposity Indicators In Infertile Females Of Reproductive Age Group. Asian J Pharm Clin Res. 2018:341-3.
Isong I, Okhormhe Z, Eze-Bassey I, Okpokam D, Usoro C. Levels of prolactin, progesterone, estradiol, luteinizing hormone and follicle stimulating hormone in infertile women in Calabar, Nigeria. Int J Reprod Contracept Obstet Gynecol. 2016;5(3):804.
Farooq R, Ullah SL, Ishaq H. Relation of serum leptin with sex hormones of obese infertile men and women. J Appl Pharm Sci. 2013;3(1):60.
Alfer J, Popovici RM, Fattahi A, Krieg J, Dittrich R, Beckmann MW, et al. Endometrial delay is found to be part of a normal individual dynamic transformation process. Arch Gynecol Obstet. 2021;304(6):1599-609.
Chakrabarti J, Chatterjee R, Goswami S, Chakravarty B, Kabir SN. Overt leptin response to controlled ovarian hyperstimulation negatively correlates with pregnancy outcome in in vitro fertilization--embryo transfer cycle. J Hum Reprod Sci. 2012;5(2):194.
Plenkin FSaD, S. J. Raised Leptin Level Can Predict Infertility in Women Failed to Conceive After 5 Years of Treatment. J Med Surg Pract. 2021;7(3):266-74.
Lin L-T, Li C-J, Tsui K-H. Serum testosterone levels are positively associated with serum anti-mullerian hormone levels in infertile women. Sci Rep. 2021;11(1):1-8.
Hernáez Á, Rogne T, Skåra KH, Håberg SE, Page CM, Fraser A, et al. Body mass index and subfertility: multivariable regression and Mendelian randomization analyses in the Norwegian Mother, Father and Child Cohort Study. Human Rep. 2021;36(12):3141-51.
Musa S, Osman S. Risk profile of Qatari women treated for infertility in a tertiary hospital: a case-control study. Fertil Res Pract. 2020;6(1):1-17.
Al Maskari MY, Alnaqdy AA. Correlation between serum leptin levels, body mass index and obesity in Omanis. Sultan Qaboos Univ Med J. 2006;6(2):27.
Zuhair Abdul-Majeed Alkhwaja, S., Jabir Edan, B., & Raad Muhi, Z. (2022). Role of Serum Leptin Levels in Women with Primary Subfertility. Archives of Razi Institute, 77(5), 1699-1707. doi: 10.22092/ari.2022.357806.2103
S Zuhair Abdul-Majeed Alkhwaja; B Jabir Edan; Z Raad Muhi. "Role of Serum Leptin Levels in Women with Primary Subfertility". Archives of Razi Institute, 77, 5, 2022, 1699-1707. doi: 10.22092/ari.2022.357806.2103
Zuhair Abdul-Majeed Alkhwaja, S., Jabir Edan, B., Raad Muhi, Z. (2022). 'Role of Serum Leptin Levels in Women with Primary Subfertility', Archives of Razi Institute, 77(5), pp. 1699-1707. doi: 10.22092/ari.2022.357806.2103
Zuhair Abdul-Majeed Alkhwaja, S., Jabir Edan, B., Raad Muhi, Z. Role of Serum Leptin Levels in Women with Primary Subfertility. Archives of Razi Institute, 2022; 77(5): 1699-1707. doi: 10.22092/ari.2022.357806.2103