Comparative Genetic Features of S1 Gene from Six Massachusetts and Variant Avian 2 Infectious Bronchitis Viruses Isolated in Iran

Document Type : Original Articles

Authors

1 Department of Poultry Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Department of Poultry Disease Research and Diagnostic, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Infectious bronchitis (IB) disease, avian Infectious Bronchitis disease in one of the major cause of respiratory problems and economic loss in poultry industry, even in developed countries with good biosecurity practice. Since the first isolation of the virus in 1931, a lot of serotypes and genotypes of the virus have been reported around the world. The GI-1 lineage, including Massachusetts (Mass) serotype viruses, is one of the most widely spread types worldwide. Moreover, the GI-23 lineage with a growing incidence rate was reported approximately 20 years ago in the Middle East, with no or little homologues vaccine use. The genotype was previously restricted to the Middle East; now, there is evidence that it has spread to European countries, raising concerns regarding potential outbreaks. In the present study, our attempt was to phylogenetically analyze the S1 gene of six isolates from Massachusetts and variant 2 genotypes, which were isolated from broiler and broiler breeder flocks in Iran. The variant 2 viruses were compared to other reported variant 2 viruses from neighboring countries and they had more than 98% identity with the latest reported Iranian variant 2. In addition, Three Mass type viruses were similar to vaccine strains which may be shows continuous circulation of vaccine viruses in the field. This event can cause increasing the risk of their mutation or even reversion to virulence after several passages in natural host, furthermore circulating viruses may recombinant with virulent field viruses and cause emergence of new variants. Considering the variable nature of IB viruses in which few changes lead to important differences, continuous epidemiological surveillance along with clinical studies of new isolates, are crucial to a better understanding of their pathogenicity and subsequent disease control.

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Main Subjects

References

  1. de Wit JJ, Cook JKA, van der Heijden HMJF. Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol. 2011;40(3):223-35.
  2. Rahman MM, Talukder A, Chowdhury MMH, Talukder R, Akter R. Coronaviruses in wild birds – A potential and suitable vector for global distribution. Vet Med Sci. 2021;7(1):264-72.
  3. Wille M, Holmes EC. Wild birds as reservoirs for diverse and abundant gamma- and deltacoronaviruses. FEMS Microbiol Rev. 2020;44(5):631-44.
  4. De Wit JJ. Detection of infectious bronchitis virus. Avian Pathol. 2000;29(2):71-93.
  5. Lin S-Y, Chen H-W. Infectious Bronchitis Virus Variants: Molecular Analysis and Pathogenicity Investigation. 2017;18(10):2030.
  6. Cavanagh D. Coronaviruses in poultry and other birds. Avian Pathology. 2005;34(6):439-48.
  7. Lee C-W, Hilt DA, Jackwood MW. Typing of Field Isolates of Infectious Bronchitis Virus Based on the Sequence of the Hypervariable Region in the S1 Gene. J Vet Diagn Invest. 2003;15(4):344-8.
  8. Montassier H. Molecular epidemiology and evolution of avian infectious bronchitis virus. Braz J Poult Sci. 2010;12(2):87-96.
  9. Al-Jallad T, Kassouha M, Salhab M, Alomar A, Al-Masalma M, Abdelaziz F. Molecular characterization of isolated infectious bronchitis viruses from affected vaccinated broiler flocks in Syria. BMC Vet Res. 2020;16(1):449.
  10. Loa CC, Lin TL, Wu CC, Bryan TA, Hooper TA, Schrader DL. Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction. J Virol Methods. 2006;131(1):86-91.
  11. Lisowska A, Sajewicz-Krukowska J, Fusaro A, PikuBa A, DombDska-Blicharz K. First characterization of a Middle-East GI-23 lineage (Var2-like) of infectious bronchitis virus in Europe. Virus Res. 2017;242:43 - 8.
  12. Valastro V, Holmes EC, Britton P, Fusaro A, Jackwood MW, Cattoli G, et al. S1 gene-based phylogeny of infectious bronchitis virus: An attempt to harmonize virus classification. Infect Genet Evol. 2016;39:349 - 64.
  13. Jackwood M, Hilt D, Callison S, Lee C-W, Plaza H, Wade E. Spike Glycoprotein Cleavage Recognition Site Analysis of Infectious Bronchitis Virus. Avian Dis. 2001;45:366-72.
  14. Yamada Y, Liu DX. Proteolytic activation of the spike protein at a novel RRRR/S motif is implicated in furin-dependent entry, syncytium formation, and infectivity of coronavirus infectious bronchitis virus in cultured cells. J Virol. 2009;83(17):8744-58.
  15. Modiri Hamadan A, Ghalyanchilangeroudi A, Hashemzadeh M, Hosseini H, Karimi V, Yahyaraeyat R, et al. Genotyping of Avian infectious bronchitis viruses in Iran (2015–2017) reveals domination of IS-1494 like virus. Virus Res. 2017;240:101-6.
  16. Hosseini H, Fard M, Charkhkar S, Morshed R. Epidemiology of Avian Infectious Bronchitis Virus Genotypes in Iran (2010–2014). Avian Dis. 2015;59.
  17. Mousavi FS, Ghalyanchilangeroudi A, Hosseini H, Nayeri Fasaei B, Ghafouri SA, Abdollahi H, et al. Complete genome analysis of Iranian IS-1494 like avian infectious bronchitis virus. Virus Dis. 2018;29(3):390-4.
  18. Saadat Y, Bozorgmehri Fard MH, Charkhkar S, Hosseini H, Shaikhi N, Akbarpour B. Molecular characterization of infectious bronchitis viruses isolated from broiler flocks in Bushehr province, Iran: 2014 - 2015. Vet Res Forum. 2017;8(3):195-201.
  19. Najafi H, Ghalyanchilangeroudi A, Hashemzadeh M, Madadgar O, Karimi V, Farahani R, et al. Pathogenicity characteristics of an Iranian variant-2 (IS-1494) like infectious bronchitis virus in experimentally infected SPF chickens. Acta Virol. 2016;60:393-9.
  20. Fischer S, Klosterhalfen D, Wilms-Schulze Kump F, Casteel M. Research Note: First evidence of infectious bronchitis virus Middle-East GI-23 lineage (Var2-like) in Germany. Poult Sci. 2020;99(2):797-800.
  21. Franzo G, Legnardi M, Tucciarone CM, Drigo M, Martini M, Cecchinato M. Evolution of infectious bronchitis virus in the field after homologous vaccination introduction. Vet Res. 2019;50(1):92.
  1. Gholami F, Karimi V, Ghalyanchi Langeroudi A, Hashemzadeh M, Vasfi Marandi M. Genotyping of Infectious bronchitis viruses isolated from broiler chicken farms in Iran during 2015-2016 .Iran J Vet Med. 2018;12(1):9-17.
  2. Callison SA, Jackwood MW, Hilt DA. Molecular Characterization of Infectious Bronchitis Virus Isolates Foreign to the United States and Comparison with United States Isolates. Avian Dis. 2001;45(2):492-9.
  3. Amarasinghe A, De Silva Senapathi U, Abdul-Cader MS, Popowich S, Marshall F, Cork SC, et al. Comparative features of infections of two Massachusetts (Mass) infectious bronchitis virus (IBV) variants isolated from Western Canadian layer flocks. BMC Vet Res. 2018;14(1):391.
Archives of Razi Institute
Volume 77, Issue 5 - Serial Number 5
September and October 2022
Pages 1611-1619

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