Bifidobacteriaceae Family Diversity in Gut Microbiota of Patients with Renal Failure

Document Type : Original Articles


1 Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran

2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

3 Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

4 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


Bifidobacteriaceae family are gut microbiota that exhibit probiotic or health promoting effects on the host. Several studies have suggested that gut microbiota are quantitatively and qualitatively altered in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). The present study aimed to assess the members of Bifidobacteriaceae family in fecal samples of patients with CKD and ESRD and compare them with non-CKD/ESRD patients to find any changes in their counts and diversions in these patients. Twenty fresh fecal samples from patients with CKD/ESRD and twenty from non-CKD/ESRD patients were examined. Whole DNA was extracted from fecal samples and the gut microbiota composition was analyzed by next generation sequencing (NGS). A total of 651 strains were identified from 40 fecal samples, 8 (1.23%) strains of which were identified as family Bifidobacteriaceae. The most abundant species in both control and disease groups were Bifidobacterium adolescentis and Bifidobacterium longum subsp. longum, and the least abundant species in the disease group was Bifidobacterium animalis subsp. lactis. There was no significant difference in the abundance of various species between the disease and control groups (p < 0.05). This study confirms that the members of the Bifidobacteriaceae family are not altered in patients with CKD/ESRD.


Main Subjects

Article Title [French]

Diversité de la Famille des Bifidobacteriaceae dans le Microbiote Intestinal des Patients Atteints d'insuffisance Rénale

Abstract [French]

La famille des Bifidobacteriaceae est un microbiote intestinal qui présente des effets probiotiques ou favorisant la santé sur l'hôte. Plusieurs études ont suggéré que le microbiote intestinal est altéré quantitativement et qualitativement chez les patients atteints d'insuffisance rénale chronique (IRC) et d'insuffisance rénale terminale (IRT). La présente étude visait à évaluer les membres de la famille des Bifidobacteriaceae dans des échantillons fécaux de patients atteints d'IRC et d'IRT et à les comparer avec des patients non-IRC/IRT pour trouver des changements dans leurs décomptes et détournements chez ces patients. Vingt échantillons fécaux frais de patients atteints d'IRC/IRC et vingt de patients non-IRC/IRT ont été examinés. L'ADN entier a été extrait d'échantillons fécaux et la composition du microbiote intestinal a été analysée par séquençage de nouvelle génération (SNG). Au total, 651 souches ont été identifiées à partir de 40 échantillons fécaux, dont 8 (1.23%) souches ont été identifiées comme appartenant à la famille des Bifidobacteriaceae. Les espèces les plus abondantes dans les groupes témoins et malades étaient Bifidobacterium adolescentis et Bifidobacterium longum subsp. longum, et l'espèce la moins abondante dans le groupe de la maladie était Bifidobacterium animalis subsp. lactis. Il n'y avait pas de différence significative dans l'abondance des diverses espèces entre les groupes malades et témoins (p<0.05). Cette étude confirme que les membres de la famille des Bifidobacteriaceae ne sont pas altérés chez les patients atteints d'IRC/IRT.

Keywords [French]

  • Bifidobacteriaceae
  • insuffisance rénale chronique (IRC)
  • insuffisance rénale terminale (IRT)
  • séquençage de nouvelle génération (SNG)
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