Cytotoxic Effect of Biebersteinia multifida Alcoholic Extracts on MCF-7, HeLa, and A2780 Cell Lines

Document Type : Original Articles


1 Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

2 Department of Traditional Medicine, Faculty of Traditional Medicine, Kerman University of Medical Sciences, Kerman, Iran

3 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

4 Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

5 Herbal and Traditional Medicines Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


Conventional cancer treatments are costly and have different serious side effects for patients. Natural herbal treatments are widely accepted among people because of their minimal side effects, although there is little scientific knowledge about them. One of these remedies utilizes the root of Biebersteinia multifidi that has been used for years in Iran to treat different chronic genital diseases. The current study examined the effects of methanolic and ethanolic extracts of B. multifida (induction of necrosis and apoptosis) on breast cancer (MCF-7), ovarian cancer (A2780), and human cervix cancer (HeLa) cell lines in comparison with normal breast cells. These effects were determined to be morphological alterations in cell light microscopy, by flow cytometry (staining with annexin V and propidium iodide), and by measuring live cells and inhibition concentrations by MTT assay. IC50 of B. multifida on the MCF-7 cell line (methanolic extract) was 400 µg/ml and for A2780 was 250 µg/ml. The IC50 amount of B. multifida on the MCF-7 cell line (ethanolic extract) was 750 µg/ml and 1500 for A2780. Results demonstrated that apoptosis and necrosis occurred in MCF-7 and A2780 following the addition of ethanolic and methanolic extracts of B. multifida to the medium. These findings confirmed the anti-cancer effects of mehthanolic extracts of Biebersteinia multifida root and its safety for normal cells; thus, it can be applied in cancer therapy as a novel medication.


Main Subjects

Article Title [French]

Effet Cytotoxique et Nécrotique des Extraits Alcooliques de Biebersteinia multifida sur les Lignées Cellulaires MCF-7, HeLa et A2780

Abstract [French]

Les traitements conventionnels du cancer sont coûteux et ont différents effets secondaires graves pour les patients. Les traitements naturels à base de plantes sont largement acceptés par les gens en raison de leurs effets secondaires minimes, bien qu'il y ait peu de connaissances scientifiques à leur sujet. L'un de ces remèdes utilise la racine de Biebersteinia multifidi qui est utilisée depuis des années en Iran pour traiter différentes maladies génitales chroniques. La présente étude a examiné les effets des extraits méthanoliques et éthanoliques de B. multifida (induction de nécrose et d'apoptose) sur le cancer du sein (MCF-7), le cancer de l'ovaire (A2780) et le cancer du col de l'utérus humain (HeLa) en comparaison avec des cellules mammaires normales. Ces effets ont été déterminés comme étant des altérations morphologiques en microscopie optique cellulaire, par cytométrie en flux (coloration à l'annexine V et à l'iodure de propidium) et en mesurant les cellules vivantes et les concentrations d'inhibition par dosage MTT. La CI50 de B. multifida sur la lignée cellulaire MCF-7 (extrait méthanolique) était de 400 ug/ml et pour A2780 était de 250 ug/ml. La quantité de CI50 de B. multifida sur la lignée cellulaire MCF-7 (extrait éthanolique) était de 750 ug/ml et de 1500 pour A2780. Les résultats ont démontré que l'apoptose et la nécrose se sont produites dans MCF-7 et A2780 suite à l'ajout d'extraits éthanoliques et méthanoliques de B. multifida au milieu. Ces résultats ont confirmé les effets anticancéreux des extraits méthanolique de racine deBiebersteinia multifida et leur sécurité pour les cellules normales; ainsi, il peut être appliqué dans le traitement du cancer en tant que nouveau médicament.

Keywords [French]

  • Biebersteinia multifida
  • médecine traditionnelle
  • extrait
  • Mcf-7
  • Hela
  • A2780
  • Cancer
  • cytotoxicité
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