Supplementation of Plant-Based Cryopreservation Medium with Folic Acid Conserves the Quality of Bulk Post-Thawed Spermatozoa

Document Type : Original Articles

Authors

Department of Animal Science, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

The current study evaluated the effects of cryopreservation medium supplementation with folic acid as an antioxidant on post-thawed semen quality in bulk. Semen samples were collected from four proved Iranian Mahabadi bulls and diluted in extender containing 1.5% soybean lecithin. The diluted semen was assigned into six parts and supplemented with different doses of folic acid as follows: FA0 (extender without folic acid), FA0.05, FA0.1, FA0.2, FA0.4, and FA0.8 (extenders containing 0.05, 0.1, 0.2, 0.4, and 0.8 mM folic acid, respectively). Then, the semen samples were cryopreserved in liquid nitrogen. Sperm motility and velocity parameters, membrane integrity, abnormal morphology, viability, and lipid peroxidation were evaluated after thawing. In the results, FA0.05 presented higher (p≤0.05) total motility, progressive motility, membrane integrity, and viability and lower lipid peroxidation compared to other groups. Abnormal morphology was not affected (p>0.05) by treatments. In conclusion, supplementation of cryopreservation medium with 0.05 mM folic acid is a helpful method to conserve the quality of post-thawed semen in bulk.

Keywords

Main Subjects

Article Title [French]

La Supplémentation du Milieu de Cryoconservation à Base de Plantes Avec de l'acide Folique Préserve la Qualité des Spermatozoïdes en Vrac Après Décongélation

Abstract [French]

La présente étude a évalué les effets de la supplémentation en milieu de cryoconservation avec de l'acide folique en tant qu'antioxydant sur la qualité du sperme après décongélation en vrac. Des échantillons de sperme ont été prélevés sur quatre taureaux Mahabadi iraniens éprouvés et dilué dans un extenseur contenant 1.5% de lécithine de soja. Le sperme dilué a été réparti en six parties et complété par différentes doses d'acide folique comme suit: FA0 (extenseur sans acide folique), FA0.05, FA0.1, FA0.2, FA0.4 et FA0.8 (extenseurs contenant 0.05, 0.1, 0.2, 0.4 et 0.8 mM d'acide folique, respectivement). Ensuite, les échantillons de sperme ont été cryoconservés dans de l'azote liquide. Les paramètres de motilité et de vitesse des spermatozoïdes, l'intégrité de la membrane, la morphologie anormale, la viabilité et la peroxydation lipidique ont été évalués après décongélation. Dans les résultats, FA0.05 a présenté une motilité totale plus élevée (p≤0.05), une motilité progressive, une intégrité de la membrane et une viabilité et une peroxydation lipidique plus faible par rapport aux autres groupes. La morphologie anormale n'a pas été affectée (p>0.05) par les traitements. En conclusion, la supplémentation du milieu de cryoconservation avec 0.05 mM d'acide folique est une méthode utile pour conserver la qualité du sperme après décongélation en vrac.

Keywords [French]

  • vrac
  • cryoconservation
  • acide folique
  • Sperme
  • lécithine de soja

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Archives of Razi Institute
Volume 76, Issue 3
September 2021
Pages 553-559

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