Comparative evaluation of nested PCR for the rapid diagnosis of human brucellosis

Document Type: Original Articles

Authors

1 Infectious and tropical Diseases Research Center, Tabriz University of Medical Sciences,Tabriz, Iran

2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

3 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

4 Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Abstract
Background: Brucellosis is known as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective to achieve satisfying treatment results and prevent chronic infections. This study was aimed to compare the efficiency of conventional microbiological and serological approaches with nested PCR for the rapid diagnosis of human brucellosis.
Methods: A total of 120 subjects with symptoms of brucellosis were included in the study. The sensitivity and specificity of nested PCR for detection of Brucella bacteria was compared with serological and blood culture methods.
Results: Out of 120 patients enrolled, brucellosis was detected in 60.83% (73/120) of cases based on serological tests with a blood culture confirmation in 8.33% of participants. Based on results, 55% of cases were positive in serum agglutination test (SAT≥1:160), and Coombs (C-SAT≥1:160) tests. Furthermore, 7 negative SAT cases were positive in C-SAT as evidence for chronic brucellosis. Results of 2-mercaptoethanol (2-ME) ≥ 1:80 test were negative in 6 SAT-positive cases. Based on nested PCR results, 68.18% SAT positive samples were also detected by blood nested PCR and 56.06% through serum nested PCR method. The sensitivity of blood nested PCR was significantly more than serum nested PCR, SAT≥1:160 and blood culture (P

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